Differential effect of vitamins and plant growth regulators on sesquiterpene lactones and phenolic acids accumulation of Inula britannica L. shoot cultures

2021 ◽  
Author(s):  
Kalina Danova ◽  
Antoaneta Trendafilova ◽  
Milka Todorova ◽  
Miroslav Rangelov ◽  
Daniela Antonova ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Phenolic Acids ◽  
Growth Regulators ◽  
Differential Effect ◽  
Sesquiterpene Lactones ◽  
Shoot Cultures ◽  
Inula Britannica ◽  
Inula Britannica L

Effects of plant growth regulators, different culture media and strength MS on production of volatile fraction composition in shoot cultures of Ocimum basilicum

2018 ◽  
Vol 116 ◽  
pp. 231-239 ◽  
Author(s):  
Lucila Elizabeth Fragoso Monfort ◽  
Suzan Kelly Vilela Bertolucci ◽  
Andreisa Fabri Lima ◽  
Alexandre Alves de Carvalho ◽  
Aliyu Mohammed ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Culture Media ◽  
Ocimum Basilicum ◽  
Volatile Fraction ◽  
Shoot Cultures ◽  
Fraction Composition

scholarly journals LED illumination and plant growth regulators’ effects on growth and phenolic acids accumulation in Moluccella laevis L. in vitro cultures

2020 ◽  
Vol 42 (5) ◽  
Author(s):  
Sylwia Zielińska ◽  
Ewelina Piątczak ◽  
Weronika Kozłowska ◽  
Aleksandra Bohater ◽  
Anna Jezierska-Domaradzka ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Phenolic Acids ◽  
Growth Regulators ◽  
In Vitro Cultures

Role in terpenoid biogenesis of plant growth regulators in Artemisia alba Turra shoot cultures

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
K Danova ◽  
V Motyka ◽  
M Todorova ◽  
A Trendafilova ◽  
S Krumova ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Shoot Cultures

scholarly journals Effect of plant growth regulators and explants on callus induction and study of antioxidant potentials and phenolic metabolites in Salvia tebesana Bunge

2020 ◽  
Vol 44 (2) ◽  
pp. 163-173 ◽  
Author(s):  
Niloofar Hemmati ◽  
Monireh Cheniany ◽  
Ali Ganjeali
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Phenolic Acids ◽  
Growth Regulators ◽  
Callus Induction ◽  
Shoot Apical Meristem ◽  
Apical Meristem ◽  
Total Phenolic ◽  
Scale Production

This study was undertaken to investigate the effect of different plant growth regulators (PGRs) on callus induction in Salvia tebesana explants grown in vitro and to evaluate the content of secondary phenolic compounds and their antioxidant potential. The explants (shoot apical meristem, leaf and petiole) were dissected from an 8-week-old plant of S. tebesana growing in vitro and cultured on MS media containing different concentrations of 2,4-D (0, 0.5, 1, 1.5 and 2 mg L-1), NAA (0, 0.5 and 1 mg L-1) and BAP (0, 0.5 and 1 mg L-1), either alone or in a blend with each other. Morphological characteristics of the callus (consistency and colour), biomass increase based on fresh and dry weight and the percentage of induction were recorded after 56 days. Levels of total phenols, ortho-diphenols, phenolic acids, flavonoids, proanthocyanidins and flavonols of callus, as well as antioxidant activities, were evaluated in vitro. The maximum callus formation (100%) was obtained from shoot apical meristem on MS medium supplemented with 0.5 and 1.5 mg L-1 2,4-D + 1 mg L-1 BAP and with 1 and 1.5 mg L-1 2,4-D + 0.5 mg L-1 BAP, whereas the highest fresh (15.06 ? 0.88 g) and dry (0.33 ? 0.02 g) weights of call were observed in a medium containing1.5 mg L-1 2,4-D + 0.5 mg L-1 NAA. It was noted that MS media augmented with combined PGRs had the highest accumulation of polyphenols, phenolic acids and flavonoid compounds, with levels of content varying in the following order: 2,4-D + BAP > NAA + BAP > 2,4-D + NAA. Strong linear correlations were established between total phenolic content of callus extracts and results of the DPPH and FRAP assays (r2 = 0.896 and r2 = 0.946, p < 0.01, respectively). The obtained results suggest that the described method could be utilised as a tool for large-scale production of medicinal metabolites of S. tebesana by tissue culture.

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