Capillary liquid chromatography/atmospheric-pressure matrix-assisted laser desorption/ionisation ion trap mass spectrometry: a comparison with liquid chromatography/matrix-assisted laser desorption/ionisation time-of-flight and liquid chromatography/elect

2006 ◽  
Vol 20 (5) ◽  
pp. 829-836 ◽  
Author(s):  
Colin S. Creaser ◽  
Philip S. Green ◽  
Peter M. Kilby ◽  
Lucy Ratcliffe
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Atmospheric Pressure ◽  
Ion Trap ◽  
Laser Desorption ◽  
Capillary Liquid Chromatography ◽  
Ion Trap Mass Spectrometry ◽  
Capillary Liquid ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

Atmospheric Pressure Matrix-Assisted Laser Desorption/Ionisation Ion Trap Mass Spectrometry of Synthetic Polymers: A Comparison with Vacuum Matrix-Assisted Laser Desorption/Ionisation Time-of-Flight Mass Spectrometry

2003 ◽  
Vol 9 (1) ◽  
pp. 33-44 ◽  
Author(s):  
Colin S. Creaser ◽  
James C. Reynolds ◽  
Andrew J. Hoteling ◽  
William F. Nichols ◽  
Kevin G. Owens
Keyword(s):  
Mass Spectrometry ◽  
Alkali Metal ◽  
Atmospheric Pressure ◽  
Hyperbranched Polymer ◽  
Ion Trap ◽  
Metal Salt ◽  
Alkali Metal Salt ◽  
Laser Desorption ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

Atmospheric pressure matrix-assisted laser desorption/ionisation quadrupole ion trap (AP-MALDI/QIT) mass spectrometry has been investigated for the analysis of polyethylene glycol (PEG 1500) and a hyperbranched polymer (polyglycidol) in the presence of alkali–metal salts. Mass spectra of PEG 1500 obtained at atmospheric pressure showed dimetallated matrix/analyte adducts, in addition to the expected alkali–metal/PEG ions, for all matrix/alkali–metal salt combinations. The relative intensities of the desorbed ions were dependent on the matrix, the alkali–metal salt added to aid cationisation and the ion trap interface conditions [capillary temperature, in-source collisionally-induced dissociation (CID)]. These data indicate that the adducts are rapidly stabilised by collisional cooling enabling them to be transferred into the ion trap. Experiments using identical sample preparation conditions were carried out on a vacuum MALDI time-of-flight (ToF) mass spectrometer. In all cases, vacuum MALDI-ToF spectra showed only alkali–metal/PEG ions and no matrix/analyte adducts. The tandem mass spectrometry (MS/MS) capability of the ion trap has been demonstrated for a lithiated polyglycol yielding a rich fragment-ion spectrum. Analysis of the hyperbranched polymer polyglycidol by AP-MALDI/QIT reveals the characteristic ion series for these polymers as also observed under vacuum MALDI-ToF conditions.

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