Maturation of the lantibiotic subtilin: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry to monitor precursors and their proteolytic processing in crude bacterial cultures

2001 ◽  
Vol 16 (2) ◽  
pp. 103-110 ◽  
Author(s):  
Torsten Stein ◽  
Karl-Dieter Entian
Keyword(s):  
Mass Spectrometry ◽  
Laser Desorption ◽  
Proteolytic Processing ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Desorption Ionization ◽  
Bacterial Cultures ◽  
Flight Mass Spectrometry ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

scholarly journals Characterization of Cryptosporidium parvum by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

2000 ◽  
Vol 66 (11) ◽  
pp. 4720-4724 ◽  
Author(s):  
Matthew L. Magnuson ◽  
James H. Owens ◽  
Catherine A. Kelty
Keyword(s):  
Mass Spectrometry ◽  
Cryptosporidium Parvum ◽  
Time Of Flight ◽  
Laser Desorption ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Desorption Ionization ◽  
Flight Mass Spectrometry ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

ABSTRACT Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) was used to investigate whole and freeze-thawed Cryptosporidium parvum oocysts. Whole oocysts revealed some mass spectral features. Reproducible patterns of spectral markers and increased sensitivity were obtained after the oocysts were lysed with a freeze-thaw procedure. Spectral-marker patterns forC. parvum were distinguishable from those obtained forCryptosporidium muris. One spectral marker appears specific for the genus, while others appear specific at the species level. Three different C. parvum lots were investigated, and similar spectral markers were observed in each. Disinfection of the oocysts reduced and/or eliminated the patterns of spectral markers.

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