Preparation and evaluation of gellan gum hydrogel reinforced with silk fibers with enhanced mechanical and biological properties for cartilage tissue engineering

2021 ◽  
Author(s):  
Wooyoup Kim ◽  
Joo Hee Choi ◽  
Pilyun Kim ◽  
Jina Youn ◽  
Jeong Eun Song ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cartilage Tissue Engineering ◽  
Biological Properties ◽  
Gellan Gum ◽  
Cartilage Tissue ◽  
Silk Fibers ◽  
Preparation And Evaluation

Preparation and characterization of an injectable dexamethasone-cyclodextrin complexes-loaded gellan gum hydrogel for cartilage tissue engineering

2020 ◽  
Vol 327 ◽  
pp. 747-765
Author(s):  
Joo Hee Choi ◽  
Ain Park ◽  
Wonchan Lee ◽  
Jina Youn ◽  
Min A. Rim ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cartilage Tissue Engineering ◽  
Gellan Gum ◽  
Cartilage Tissue ◽  
Cyclodextrin Complexes

Advanced gellan gum-based glycol chitosan hydrogel for cartilage tissue engineering biomaterial

2020 ◽  
Vol 158 ◽  
pp. 452-460
Author(s):  
Sumi Lee ◽  
Joo Hee Choi ◽  
Ain Park ◽  
Mina Rim ◽  
Jina Youn ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cartilage Tissue Engineering ◽  
Gellan Gum ◽  
Cartilage Tissue ◽  
Glycol Chitosan ◽  
Chitosan Hydrogel

scholarly journals Gellan Gum Injectable Hydrogels for Cartilage Tissue Engineering Applications: In Vitro Studies and Preliminary In Vivo Evaluation

2010 ◽  
Vol 16 (1) ◽  
pp. 343-353 ◽  
Author(s):  
João T. Oliveira ◽  
Tírcia C. Santos ◽  
Luís Martins ◽  
Ricardo Picciochi ◽  
Alexandra P. Marques ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cartilage Tissue Engineering ◽  
Gellan Gum ◽  
In Vitro Studies ◽  
Cartilage Tissue ◽  
Engineering Applications ◽  
In Vivo Evaluation ◽  
Injectable Hydrogels

Novel injectable porous poly(γ-benzyl-l-glutamate) microspheres for cartilage tissue engineering: preparation and evaluation

2015 ◽  
Vol 3 (6) ◽  
pp. 1020-1031 ◽  
Author(s):  
Jianjun Fang ◽  
Qi Yong ◽  
Kunxi Zhang ◽  
Wentao Sun ◽  
Shifeng Yan ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Synthetic Polypeptide ◽  
Preparation And Evaluation ◽  
Porous Poly

A novel injectable synthetic polypeptide of a poly(γ-benzyl-l-glutamate) macroporous microcarrier was developed for cartilage tissue engineering.

scholarly journals Gellan gum: A new biomaterial for cartilage tissue engineering applications

2009 ◽  
Vol 9999A ◽  
pp. NA-NA ◽  
Author(s):  
J. T. Oliveira ◽  
L. Martins ◽  
R. Picciochi ◽  
P. B. Malafaya ◽  
R. A. Sousa ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cartilage Tissue Engineering ◽  
Gellan Gum ◽  
Cartilage Tissue ◽  
Engineering Applications

scholarly journals Preparation and evaluation of the electrospun chitosan/PEO fibers for potential applications in cartilage tissue engineering

2005 ◽  
Vol 16 (7) ◽  
pp. 861-873 ◽  
Author(s):  
Anuradha Subramanian ◽  
David Vu ◽  
Gustavo F. Larsen ◽  
Hsin-Yi Lin
Keyword(s):  
Tissue Engineering ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Potential Applications ◽  
Preparation And Evaluation

scholarly journals Development and Evaluation of Gellan Gum/Silk Fibroin/Chondroitin Sulfate Ternary Injectable Hydrogel for Cartilage Tissue Engineering

Biomolecules ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1184
Author(s):  
Seongwon Lee ◽  
Joohee Choi ◽  
Jina Youn ◽  
Younghun Lee ◽  
Wooyoup Kim ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Chondroitin Sulfate ◽  
Silk Fibroin ◽  
Articular Chondrocytes ◽  
Cartilage Tissue Engineering ◽  
Cell Encapsulation ◽  
Gellan Gum ◽  
Cartilage Tissue

Hydrogel is in the spotlight as a useful biomaterial in the field of drug delivery and tissue engineering due to its similar biological properties to a native extracellular matrix (ECM). Herein, we proposed a ternary hydrogel of gellan gum (GG), silk fibroin (SF), and chondroitin sulfate (CS) as a biomaterial for cartilage tissue engineering. The hydrogels were fabricated with a facile combination of the physical and chemical crosslinking method. The purpose of this study was to find the proper content of SF and GG for the ternary matrix and confirm the applicability of the hydrogel in vitro and in vivo. The chemical and mechanical properties were measured to confirm the suitability of the hydrogel for cartilage tissue engineering. The biocompatibility of the hydrogels was investigated by analyzing the cell morphology, adhesion, proliferation, migration, and growth of articular chondrocytes-laden hydrogels. The results showed that the higher proportion of GG enhanced the mechanical properties of the hydrogel but the groups with over 0.75% of GG exhibited gelling temperatures over 40 °C, which was a harsh condition for cell encapsulation. The 0.3% GG/3.7% SF/CS and 0.5% GG/3.5% SF/CS hydrogels were chosen for the in vitro study. The cells that were encapsulated in the hydrogels did not show any abnormalities and exhibited low cytotoxicity. The biochemical properties and gene expression of the encapsulated cells exhibited positive cell growth and expression of cartilage-specific ECM and genes in the 0.5% GG/3.5% SF/CS hydrogel. Overall, the study of the GG/SF/CS ternary hydrogel with an appropriate content showed that the combination of GG, SF, and CS can synergistically promote articular cartilage defect repair and has considerable potential for application as a biomaterial in cartilage tissue engineering.

scholarly journals Review of Synthetic and Hybrid Scaffolds in Cartilage Tissue Engineering

Membranes ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 348
Author(s):  
Monika Wasyłeczko ◽  
Wioleta Sikorska ◽  
Andrzej Chwojnowski
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Hybrid Materials ◽  
Cartilage Tissue Engineering ◽  
Biological Properties ◽  
General Information ◽  
Cartilage Tissue ◽  
Cartilage Defects ◽  
Natural Polymers ◽  
Cellular Environment

Cartilage tissue is under extensive investigation in tissue engineering and regenerative medicine studies because of its limited regenerative potential. Currently, many scaffolds are undergoing scientific and clinical research. A key for appropriate scaffolding is the assurance of a temporary cellular environment that allows the cells to function as in native tissue. These scaffolds should meet the relevant requirements, including appropriate architecture and physicochemical and biological properties. This is necessary for proper cell growth, which is associated with the adequate regeneration of cartilage. This paper presents a review of the development of scaffolds from synthetic polymers and hybrid materials employed for the engineering of cartilage tissue and regenerative medicine. Initially, general information on articular cartilage and an overview of the clinical strategies for the treatment of cartilage defects are presented. Then, the requirements for scaffolds in regenerative medicine, materials intended for membranes, and methods for obtaining them are briefly described. We also describe the hybrid materials that combine the advantages of both synthetic and natural polymers, which provide better properties for the scaffold. The last part of the article is focused on scaffolds in cartilage tissue engineering that have been confirmed by undergoing preclinical and clinical tests.

Sign in / Sign up

Export Citation Format

Share Document