Group II Nucleopolyhedrovirus Subgroups Revealed by Phylogenetic Analysis of Polyhedrin and DNA Polymerase Gene Sequences

1999 ◽  
Vol 73 (1) ◽  
pp. 59-73 ◽  
Author(s):  
Dieter M. Bulach ◽  
C.Ananth Kumar ◽  
Angelo Zaia ◽  
Bufeng Liang ◽  
David E. Tribe
2002 ◽  
Vol 76 (19) ◽  
pp. 10056-10059 ◽  
Author(s):  
Mária Benkő ◽  
Péter Élő ◽  
Krisztina Ursu ◽  
Winfried Ahne ◽  
Scott E. LaPatra ◽  
...  

ABSTRACT From adenovirus-like viruses originating from a fish and a snake species, a conserved part of the adenoviral DNA polymerase gene was PCR amplified, cloned and sequenced. Phylogenetic analysis showed that the snake adenovirus is closely related to the members of the proposed genus Atadenovirus, whereas the fish isolate seems to represent a separate cluster, likely a new genus.


Virus Genes ◽  
2005 ◽  
Vol 30 (3) ◽  
pp. 371-381 ◽  
Author(s):  
Maria Cardoso ◽  
Alex Hyatt ◽  
Paul Selleck ◽  
Sue Lowther ◽  
Vibhu Prakash ◽  
...  

Author(s):  
William H. Wilson ◽  
Declan C. Schroeder ◽  
Jenna Ho ◽  
Martin Canty

A new virus that infects the harmful algal bloom-forming microalga Phaeocystis globosa was isolated from surface water in the English Channel off the coast of Plymouth, UK, in May 2001. Phylogenetic analysis of the DNA polymerase gene revealed the virus isolate, designated PgV-102P, belongs to the family Phycodnaviridae, a group of large double-stranded DNA viruses known to infect algae. Basic characterization of PgV-102P revealed it was a lytic virus with a relatively slow culture lysis period of 10-days. The genome size (176 kbp) and capsid diameter (98 nm) of PgV-102P fall at the bottom end of the range expected for phycodnaviruses. Interestingly, PgV-102P did not cluster with other P. globosa viruses; instead, it was more closely related to other prymnesioviruses that infect the marine prymnesiophyte Chrysochromulina brevifilum. We discuss the effectiveness of DNA polymerase as a diagnostic marker. Although it is ideal for determining what family or even genus an algal virus belongs to, it is clear that the DNA polymerase gene does not have sufficient resolution when looking for relationships within algal virus genera.


2010 ◽  
Vol 4 (10) ◽  
pp. 1243-1251 ◽  
Author(s):  
Sijun Huang ◽  
Steven W Wilhelm ◽  
Nianzhi Jiao ◽  
Feng Chen

2009 ◽  
Vol 75 (11) ◽  
pp. 3634-3640 ◽  
Author(s):  
Jessica M. Labonté ◽  
Karen E. Reid ◽  
Curtis A. Suttle

ABSTRACT The distribution of viral genotypes in the ocean and their evolutionary relatedness remain poorly constrained. This paper presents data on the genetic diversity and evolutionary relationships of 1.2-kb DNA polymerase (pol) gene fragments from podoviruses. A newly designed set of PCR primers was used to amplify DNA directly from coastal sediment and water samples collected from inlets adjacent to the Strait of Georgia, British Columbia, Canada, and from the northeastern Gulf of Mexico. Restriction fragment length polymorphism analysis of 160 cloned PCR products revealed 29 distinct operational taxonomic units (OTUs), with OTUs within a site typically being more similar than those among sites. Phylogenetic analysis of the DNA pol gene fragments demonstrated high similarity between some environmental sequences and sequences from the marine podoviruses roseophage SIO1 and cyanophage P60, while others were not closely related to sequences from cultured phages. Interrogation of the CAMERA database for sequences from metagenomics data demonstrated that the amplified sequences were representative of the diversity of podovirus pol sequences found in marine samples. Our results indicate high genetic diversity within marine podovirus communities within a small geographic region and demonstrate that the diversity of environmental polymerase gene sequences for podoviruses is far more extensive than previously recognized.


Virology ◽  
1996 ◽  
Vol 216 (1) ◽  
pp. 146-157 ◽  
Author(s):  
BRETT J. PELLOCK ◽  
ALBERT LU ◽  
RICHARD B. MEAGHER ◽  
MICHAEL J. WEISE ◽  
LOIS K. MILLER

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