Prevalence of Microplastics in the Eastern Oyster Crassostrea virginica in the Chesapeake Bay: The Impact of Different Digestion Methods on Microplastic Properties

This study aimed to determine the microplastic prevalence in eastern oysters (C. virginica) in three sites in the Chesapeake Bay in Virginia and optimize the digestion methods. The digestion results illustrate that the lowest recovery rate and digestion recovery were related to enzymatic, enzymatic + hydrogen peroxide (H2O2), and HCl 5% treatments, while the highest digestion recovery and recovery rate were observed in H2O2 and basic (KOH) treatments. Nitric acid digestion resulted in satisfying digestion recovery (100%), while no blue polyethylene microplastics were observed due to the poor recovery rate. In addition, nitric acid altered the color, changed the Raman spectrum intensity, and melted polypropylene (PP) and polyethylene terephthalate (PET). In order to determine the number of microplastics, 144 oysters with an approximately similar size and weight from three sites, including the James River, York River, and Eastern Shore, were evaluated. Fragments were the most abundant microplastics among the different microplastics, followed by fibers and beads, in the three sites. A significantly higher number of fragments were found in the James River, probably due to the greater amount of human activities. The number of microplastics per gram of oyster tissue was higher in the James River, with 7 MPs/g tissue, than in the York River and Eastern Shore, with 6.7 and 5.6 MPs/g tissue.

Marine Heatwaves in the Chesapeake Bay

Prolonged events of anomalously warm sea water temperature, or marine heatwaves (MHWs), have major detrimental effects to marine ecosystems and the world's economy. While frequency, duration and intensity of MHWs have been observed to increase in the global oceans, little is known about their potential occurrence and variability in estuarine systems due to limited data in these environments. In the present study we analyzed a novel data set with over three decades of continuous in situ temperature records to investigate MHWs in the largest and most productive estuary in the US: the Chesapeake Bay. MHWs occurred on average twice per year and lasted 11 days, resulting in 22 MHW days per year in the bay. Average intensities of MHWs were 3°C, with maximum peaks varying between 6 and 8°C, and yearly cumulative intensities of 72°C × days on average. Large co-occurrence of MHW events was observed between different regions of the bay (50–65%), and also between Chesapeake Bay and the Mid-Atlantic Bight (40–50%). These large co-occurrences, with relatively short lags (2–5 days), suggest that coherent large-scale air-sea heat flux is the dominant driver of MHWs in this region. MHWs were also linked to large-scale climate modes of variability: enhancement of MHW days in the Upper Bay were associated with the positive phase of Niño 1+2, while enhancement and suppression of MHW days in both the Mid and Lower Bay were associated with positive and negative phases of North Atlantic Oscillation, respectively. Finally, as a result of long-term warming of the Chesapeake Bay, significant trends were detected for MHW frequency, MHW days and yearly cumulative intensity. If these trends persist, by the end of the century the Chesapeake Bay will reach a semi-permanent MHW state, when extreme temperatures will be present over half of the year, and thus could have devastating impacts to the bay ecosystem, exacerbating eutrophication, increasing the severity of hypoxic events, killing benthic communities, causing shifts in species composition and decline in important commercial fishery species. Improving our basic understanding of MHWs in estuarine regions is necessary for their future predictability and to guide management decisions in these valuable environments.

Impacts of row spacing and fungicide timing on foliar disease, greenstem and yield in double cropped soybeans grown in the Chesapeake Bay region of the United States.

Double cropped soybeans are planted on approximately 1/3 of crop acres in the Chesapeake Bay region of the United States. Producers have asked if foliar fungicides are required to optimize yields in this region. We assessed the impacts of foliar fungicide application timing and row spacing on foliar disease, greenstem, and yield from 11 site years spanning 2017-2019. Foliar diseases only developed at rateable levels in one location. Fungicide application, regardless of timing, increased percent greenstem over non-treated controls. Fungicide application did not impact soybean yield. Yield was greater in 38.1 cm rows when compared to 19 cm rows. Our data do not support the use of foliar fungicides in double cropped soybean production in this region.