Production of a Chimeric Fibroin Light-chain Polypeptide in a Fibroin Secretion-deficient Naked Pupa Mutant of the SilkwormBombyx mori

1995 ◽  
Vol 251 (2) ◽  
pp. 217-228 ◽  
Author(s):  
Kazuyuki Mori ◽  
Kazunori Tanaka ◽  
Yoshimi Kikuchi ◽  
Miho Waga ◽  
Shou Waga ◽  
...  
Keyword(s):  
Light Chain ◽  
Fibroin Light Chain

Bom‐miR‐2805 upregulates the expression of Bombyx mori fibroin light chain gene in vivo

2019 ◽  
Vol 120 (9) ◽  
pp. 14326-14335
Author(s):  
Yanhua Chen ◽  
Tao Jiang ◽  
Zhicheng Tan ◽  
Peng Xue ◽  
Jin Xu ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Light Chain ◽  
Chain Gene ◽  
Light Chain Gene ◽  
Fibroin Light Chain

scholarly journals Biopatterning: Precise Protein Photolithography (P3 ): High Performance Biopatterning Using Silk Fibroin Light Chain as the Resist (Adv. Sci. 9/2017)

2017 ◽  
Vol 4 (9) ◽  
Author(s):  
Wanpeng Liu ◽  
Zhitao Zhou ◽  
Shaoqing Zhang ◽  
Zhifeng Shi ◽  
Justin Tabarini ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
Light Chain ◽  
High Performance ◽  
Fibroin Light Chain

scholarly journals Effects of RGDS sequence genetically interfused in the silk fibroin light chain protein on chondrocyte adhesion and cartilage synthesis

Biomaterials ◽  
2010 ◽  
Vol 31 (29) ◽  
pp. 7503-7511 ◽  
Author(s):  
Yusuke Kambe ◽  
Koji Yamamoto ◽  
Katsura Kojima ◽  
Yasushi Tamada ◽  
Naohide Tomita
Keyword(s):  
Silk Fibroin ◽  
Light Chain ◽  
Fibroin Light Chain ◽  
And Cartilage

Primary structure of the silk fibroin light chain determined by cDNA sequencing and peptide analysis

1989 ◽  
Vol 210 (1) ◽  
pp. 127-139 ◽  
Author(s):  
Kazunori Yamaguchi ◽  
Yoshimi Kikuchi ◽  
Takashi Takagi ◽  
Aiko Kikuchi ◽  
Fumitaka Oyama ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
Light Chain ◽  
Primary Structure ◽  
Cdna Sequencing ◽  
Peptide Analysis ◽  
Fibroin Light Chain

Structure of the Bombyx mori fibroin light-chain-encoding gene: upstream sequence elements common to the light and heavy chain

Gene ◽  
1992 ◽  
Vol 110 (2) ◽  
pp. 151-158 ◽  
Author(s):  
Yoshimi Kikuchi ◽  
Kazuyuki Mori ◽  
Satoshi Suzuki ◽  
Kazunori Yamaguchi ◽  
Shigeki Mizuno
Keyword(s):  
Bombyx Mori ◽  
Heavy Chain ◽  
Light Chain ◽  
Upstream Sequence ◽  
Sequence Elements ◽  
Encoding Gene ◽  
Fibroin Light Chain

scholarly journals Allelic variability in the third intron of the fibroin light chain gene in Bombyx mori (Lepidoptera: Bombycidae)

2009 ◽  
Vol 8 (1) ◽  
pp. 197-206 ◽  
Author(s):  
J.F. Barbosa ◽  
J.P. Bravo ◽  
D.B. Zanatta ◽  
J.L.C. Silva ◽  
M.A. Fernandez
Keyword(s):  
Bombyx Mori ◽  
Light Chain ◽  
Chain Gene ◽  
Light Chain Gene ◽  
The Third ◽  
Allelic Variability ◽  
Fibroin Light Chain

scholarly journals Loss of Posterior Silk Gland Transcription Specificity of Fibroin Light Chain Promoter due to Absence of 41 bp Sequence Containing Possible Inhibitor Binding Sites

2005 ◽  
Vol 37 (12) ◽  
pp. 819-825 ◽  
Author(s):  
Ting-Qing Guo ◽  
Jian-Yang Wang ◽  
Sheng-Peng Wang ◽  
Xiu-Yang Guo ◽  
Ke-Wei Huang ◽  
...  
Keyword(s):  
Reporter Gene ◽  
Light Chain ◽  
Binding Sites ◽  
Fluorescent Protein ◽  
Silk Gland ◽  
Inhibitor Binding ◽  
Egfp Reporter Gene ◽  
Egfp Reporter ◽  
Fibroin Light Chain ◽  
Posterior Silk Gland

Abstract The gene encoding fibroin light chain protein (FibL) is specifically expressed in the posterior silk gland of silkworm and repressed in other tissues. The binding sites of several transcription factors involved in the silk gland transcription specificity of fibl promoter have been recognized, including SGFB, PSGF and BMFA. Here we report the leak expression of the enhanced green fluorescent protein (EGFP) reporter gene in tissues other than the posterior silk gland in vivo when under the control of a shortened fibl promoter with deletion of the 5′ terminal 41 bp sequence, which is located at −650 nt to −610 nt upstream of the fibl transcription starting site. Assay of silk gland specificity of the promoters was performed by observation of green fluorescence in tissues of silkworm larvae following inter-haemocoelic injection of recombinant Autographa californica multiple nuclear polyhedrosis virus carrying the EGFP reporter gene controlled by different lengths of fibl promoters. Our results indicated that availability of the binding sites of several known factors, including SGFB, PSGF and BMFA, is not sufficient for intact silk gland transcription specificity of fibl promoter, and there are possible inhibitor binding sites in the 41 bp sequence (−650 nt to −610 nt) upstream of the transcription starting site which may be required to repress the activity of fibl promoter in other tissues.

scholarly journals Linkage analysis of the fibroin light chain gene in the silkworm, Bombyx mori.

1984 ◽  
Vol 59 (3) ◽  
pp. 285-296 ◽  
Author(s):  
Akio HYODO ◽  
Toshio YAMAMOTO ◽  
Hitoshi UEDA ◽  
Fusaho TAKEI ◽  
Ken-ichi KIMURA ◽  
...  
Keyword(s):  
Linkage Analysis ◽  
Bombyx Mori ◽  
Light Chain ◽  
Chain Gene ◽  
Light Chain Gene ◽  
Fibroin Light Chain ◽  
Silkworm Bombyx Mori
Sign in / Sign up

Export Citation Format

Share Document