Vibrational Raman Optical Activity of α-Lactalbumin: Comparison with Lysozyme, and Evidence for Native Tertiary Folds in Molten Globule States

1995 ◽  
Vol 254 (4) ◽  
pp. 747-760 ◽  
Author(s):  
Gary Wilson ◽  
Steven J. Ford ◽  
Alan Cooper ◽  
Lutz Hecht ◽  
Zai Q. Wen ◽  
...  
Keyword(s):  
Optical Activity ◽  
Molten Globule ◽  
Raman Optical Activity

Raman optical activity characterization of native and molten globule states of equine lysozyme: Comparison with hen lysozyme and bovine α-lactalbumin

Biopolymers ◽  
2000 ◽  
Vol 57 (4) ◽  
pp. 235-248 ◽  
Author(s):  
Ewan W. Blanch ◽  
Ludmilla A. Morozova-Roche ◽  
Lutz Hecht ◽  
Wim Noppe ◽  
Laurence D. Barron
Keyword(s):  
Optical Activity ◽  
Molten Globule ◽  
Raman Optical Activity

scholarly journals Raman optical activity of humanα-synuclein in intrinsically disordered, micelle-boundα-helical, molten globule and oligomericβ-sheet state

2017 ◽  
Vol 48 (7) ◽  
pp. 910-918 ◽  
Author(s):  
Carl Mensch ◽  
Albert Konijnenberg ◽  
Roos Van Elzen ◽  
Anne-Marie Lambeir ◽  
Frank Sobott ◽  
...  
Keyword(s):  
Optical Activity ◽  
Molten Globule ◽  
Intrinsically Disordered ◽  
Raman Optical Activity

Recent Developments in Raman Optical Activity of Biopolymers

1996 ◽  
Vol 50 (5) ◽  
pp. 619-629 ◽  
Author(s):  
L. D. Barron ◽  
L. Hecht ◽  
A. F. Bell ◽  
G. Wilson
Keyword(s):  
Secondary Structure ◽  
Nucleic Acids ◽  
Optical Activity ◽  
Solution Structure ◽  
Tertiary Structure ◽  
Molten Globule ◽  
Raman Optical Activity ◽  
Recent Developments ◽  
Different Temperatures ◽  
Carbohydrate Components

Recent advances in Raman optical activity (ROA) instrumentation are outlined which have enhanced significantly the quality of vibrational ROA spectra of biopolymers in aqueous solution. Peptides, proteins, carbohydrates, glycoproteins, and nucleic acids now provide excellent ROA spectra which contain detailed information about solution structure. ROA spectra can be measured just as easily in D2O as in H2O solution, and, as illustrated for bovine serum albumin and concanavalin A, a comparison of the two can be highly informative. In addition to signatures of extended secondary structure, protein ROA spectra also contain signatures related to loops and turns which are valuable for studying tertiary structure and dynamics, exemplified here by a comparison of the ROA spectra of reduced lysozyme and unordered poly-L-lysine, by the ROA spectra of acid molten globule α-lactalbumin at different temperatures, which reveal a native-like tertiary fold, and by changes in the ROA spectrum of native lysozyme on binding to a saccharide inhibitor. Carbohydrate ROA spectra contain signatures of all the central features of their stereochemistry and, as shown by a comparison of laminaribiose with laminarin, can also probe extended secondary structure in polysaccharides. Results on a glycoprotein, orosomucoid, suggest that ROA can provide information about both the protein and the carbohydrate components. Preliminary results on nucleic acids are outlined with the ROA spectra of Poly(rA)·Poly(rU) and Poly(rI)·Poly(rC) shown as examples.

Structural characterization of proteins and viruses using Raman optical activity

2004 ◽  
Vol 35 (1-2) ◽  
pp. 87-92 ◽  
Author(s):  
Ewan W Blanch ◽  
Iain H McColl ◽  
Lutz Hecht ◽  
Kurt Nielsen ◽  
Laurence D Barron
Keyword(s):  
Optical Activity ◽  
Structural Characterization ◽  
Raman Optical Activity

scholarly journals Recognition of the true and false resonance Raman optical activity

2021 ◽  
Author(s):  
Malgorzata Baranska ◽  
Ewa Machalska ◽  
Grzegorz Zajac ◽  
Aleksandra J. Wierzba ◽  
Josef Kapitán ◽  
...  
Keyword(s):  
Optical Activity ◽  
Resonance Raman ◽  
Raman Optical Activity

scholarly journals Recognition of the true and false resonance Raman optical activity

2021 ◽  
Author(s):  
Malgorzata Baranska ◽  
Ewa Machalska ◽  
Grzegorz Zajac ◽  
Aleksandra J. Wierzba ◽  
Josef Kapitán ◽  
...  
Keyword(s):  
Optical Activity ◽  
Resonance Raman ◽  
Raman Optical Activity

scholarly journals Raman Optical Activity Probing the Active Site of Chromoproteins

2014 ◽  
Vol 54 (1) ◽  
pp. 039-042 ◽  
Author(s):  
Masashi UNNO
Keyword(s):  
Optical Activity ◽  
Active Site ◽  
Raman Optical Activity

Valence optical theory of vibrational circular dichroism and Raman optical activity

1988 ◽  
Vol 65 (2) ◽  
pp. 327-344 ◽  
Author(s):  
Juan R. Escribano ◽  
Laurence D. Barron
Keyword(s):  
Circular Dichroism ◽  
Optical Activity ◽  
Vibrational Circular Dichroism ◽  
Raman Optical Activity ◽  
Optical Theory ◽  
Circular Dichroïsm

Methyl torsion Raman optical activity in trans-2,3-dimethyloxirane and trans-2,3-dimethylthiirane

1992 ◽  
Vol 48 (8) ◽  
pp. 1193-1195 ◽  
Author(s):  
L.D. Barron ◽  
L. Hecht ◽  
P.L. Polavarapu
Keyword(s):  
Optical Activity ◽  
Raman Optical Activity ◽  
In Trans ◽  
Methyl Torsion

Raman Optical Activity Probing Structural Deformations of the 4-Hydroxycinnamyl Chromophore in Photoactive Yellow Protein

2013 ◽  
Vol 4 (8) ◽  
pp. 1322-1327 ◽  
Author(s):  
Takahito Shingae ◽  
Kensuke Kubota ◽  
Masato Kumauchi ◽  
Fumio Tokunaga ◽  
Masashi Unno
Keyword(s):  
Optical Activity ◽  
Photoactive Yellow Protein ◽  
Raman Optical Activity ◽  
Structural Deformations
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