Structural characterization of proteins and viruses using Raman optical activity

2004 ◽  
Vol 35 (1-2) ◽  
pp. 87-92 ◽  
Author(s):  
Ewan W Blanch ◽  
Iain H McColl ◽  
Lutz Hecht ◽  
Kurt Nielsen ◽  
Laurence D Barron
Keyword(s):  
Optical Activity ◽  
Structural Characterization ◽  
Raman Optical Activity

Use of Raman and Raman optical activity for the structural characterization of a therapeutic monoclonal antibody formulation subjected to heat stress

2015 ◽  
Vol 46 (6) ◽  
pp. 531-536 ◽  
Author(s):  
Geetha Thiagarajan ◽  
Effendi Widjaja ◽  
Jun Hyuk Heo ◽  
Jason K. Cheung ◽  
Busolo Wabuyele ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Heat Stress ◽  
Optical Activity ◽  
Structural Characterization ◽  
Therapeutic Monoclonal Antibody ◽  
Raman Optical Activity

Raman optical activity characterization of native and molten globule states of equine lysozyme: Comparison with hen lysozyme and bovine α-lactalbumin

Biopolymers ◽  
2000 ◽  
Vol 57 (4) ◽  
pp. 235-248 ◽  
Author(s):  
Ewan W. Blanch ◽  
Ludmilla A. Morozova-Roche ◽  
Lutz Hecht ◽  
Wim Noppe ◽  
Laurence D. Barron
Keyword(s):  
Optical Activity ◽  
Molten Globule ◽  
Raman Optical Activity

Vibrational Raman Optical Activity Characterization of Poly(l-proline) II Helix in Alanine Oligopeptides

2004 ◽  
Vol 126 (16) ◽  
pp. 5076-5077 ◽  
Author(s):  
Iain H. McColl ◽  
Ewan W. Blanch ◽  
Lutz Hecht ◽  
Neville R. Kallenbach ◽  
Laurence D. Barron
Keyword(s):  
Optical Activity ◽  
Raman Optical Activity

Vibrational optical activity for structural characterization of natural products

2020 ◽  
Vol 37 (12) ◽  
pp. 1661-1699 ◽  
Author(s):  
Prasad L. Polavarapu ◽  
Ernesto Santoro
Keyword(s):  
Natural Products ◽  
Optical Activity ◽  
Structural Characterization ◽  
Recent Progress

This review presents the recent progress towards elucidating the structures of chiral natural products and applications using vibrational optical activity (VOA) spectroscopy.

A Short-Wavelength Raman Optical Activity Spectrometer with Laser Source at 457 nm for the Characterization of Chiral Molecules

2017 ◽  
Vol 71 (9) ◽  
pp. 2211-2217 ◽  
Author(s):  
Ying Zhang ◽  
Peng Wang ◽  
Guoqing Jia ◽  
Feng Cheng ◽  
Zhaochi Feng ◽  
...  
Keyword(s):  
Optical Activity ◽  
Short Wavelength ◽  
Laser Source ◽  
Chiral Molecules ◽  
Raman Optical Activity

New ultrastructural findings about Borrelia burgdorferi by cryofixation, negative staining, thin sectioning and scanning techniques

1990 ◽  
Vol 48 (3) ◽  
pp. 582-583
Author(s):  
S. F. Hayes ◽  
M. D. Corwin ◽  
T. G. Schwan ◽  
D. W. Dorward ◽  
W. Burgdorfer
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Structural Characterization ◽  
Negative Staining ◽  
Low Speed ◽  
Thin Sectioning ◽  
Ultrastructural Findings

Characterization of Borrelia burgdorferi strains by means of negative staining EM has become an integral part of many studies related to the biology of the Lyme disease organism. However, relying solely upon negative staining to compare new isolates with prototype B31 or other borreliae is often unsatisfactory. To obtain more satisfactory results, we have relied upon a correlative approach encompassing a variety EM techniques, i.e., scanning for topographical features and cryotomy, negative staining and thin sectioning to provide a more complete structural characterization of B. burgdorferi.For characterization, isolates of B. burgdorferi were cultured in BSK II media from which they were removed by low speed centrifugation. The sedimented borrelia were carefully resuspended in stabilizing buffer so as to preserve their features for scanning and negative staining. Alternatively, others were prepared for conventional thin sectioning and for cryotomy using modified procedures. For thin sectioning, the fixative described by Ito, et al.

Structural characterization of the PPIase domain of FKBP51, a cochaperone of human Hsp90

2011 ◽  
Vol 44 (06) ◽  
Author(s):  
A Bracher ◽  
C Kozany ◽  
AK Thost ◽  
F Hausch
Keyword(s):  
Structural Characterization ◽  
Ppiase Domain

Structural characterization of dendrobine-type sesquiterpene alkaloids from the stems of Dendrobium nobile using LC-MS/MS

Planta Medica ◽  
2014 ◽  
Vol 80 (10) ◽  
Author(s):  
YH Wang ◽  
B Avula ◽  
N Abe ◽  
F Wei ◽  
M Wang ◽  
...  
Keyword(s):  
Structural Characterization ◽  
Dendrobium Nobile
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