Expression, Purification, and Biophysical Characterization of the BRCT Domain of Human DNA Ligase IIIα

2001 ◽  
Vol 21 (3) ◽  
pp. 401-411 ◽  
Author(s):  
Kevin H. Thornton ◽  
V.V. Krishnan ◽  
Mary G. West ◽  
Jennifer Popham ◽  
Melissa Ramirez ◽  
...  
Keyword(s):  
Dna Ligase ◽  
Brct Domain ◽  
Biophysical Characterization ◽  
Human Dna

Biophysical characterization of the interaction between FAAP20-UBZ4 domain and Rev1-BRCT domain

FEBS Letters ◽  
2015 ◽  
Vol 589 (20PartB) ◽  
pp. 3037-3043 ◽  
Author(s):  
Kyungeun Lim ◽  
Mi-Kyung Lee ◽  
Phuong T.M. Duong ◽  
Dinan Liu ◽  
Sieun Sung ◽  
...  
Keyword(s):  
Brct Domain ◽  
Biophysical Characterization

Solution Structure and Backbone Dynamics of the Human DNA Ligase IIIα BRCT Domain†,‡

Biochemistry ◽  
2001 ◽  
Vol 40 (44) ◽  
pp. 13158-13166 ◽  
Author(s):  
V. V. Krishnan ◽  
Kevin H. Thornton ◽  
Michael P. Thelen ◽  
Monique Cosman
Keyword(s):  
Solution Structure ◽  
Backbone Dynamics ◽  
Dna Ligase ◽  
Brct Domain ◽  
Human Dna

scholarly journals Characterization of human DNA ligase I expressed in a baculovirus-insect cell system

1995 ◽  
Vol 312 (2) ◽  
pp. 593-597 ◽  
Author(s):  
A Gallina ◽  
F Rossi ◽  
G Milanesi ◽  
R Rossi ◽  
A Montecucco ◽  
...  
Keyword(s):  
Expression System ◽  
Recombinant Baculovirus ◽  
Baculovirus Expression System ◽  
Cell System ◽  
Biologically Active ◽  
Dna Ligase ◽  
Human Dna ◽  
Dna Ligase I ◽  
Baculovirus System

The baculovirus expression system was used to overexpress human DNA ligase I (hLig I). Approx. 2 mg of recombinant hLig I was produced per 10(8) Spodoptera frugiperda Sf9 insect cells infected with the recombinant baculovirus. The optimum time point for the production of biologically active recombinant hLig I was 48 h post-infection. Lig I activity was demonstrated by auto-adenylating, polynucleotide joining and DNA relaxation assays. The baculovirus system has the advantage over previously described methods for producing hLig I of generating large amounts of a full-length active protein.

Purification and Biophysical Characterization of an 11S Globulin from Wrightia tinctoria Exhibiting Hemagglutinating Activity

2013 ◽  
Vol 20 (5) ◽  
pp. 499-509 ◽  
Author(s):  
Pramod Kumar ◽  
Dipak N. Patil ◽  
Anshul Chaudhary ◽  
Shailly Tomar ◽  
Dinesh Yernool ◽  
...  
Keyword(s):  
Hemagglutinating Activity ◽  
Biophysical Characterization ◽  
11S Globulin ◽  
Wrightia Tinctoria

Biophysical Characterization of Glycodendrimers As Nano-carriers for HIV Peptides

2013 ◽  
Vol 20 (31) ◽  
pp. 3935-3943 ◽  
Author(s):  
M. Ionov ◽  
K. Ciepluch ◽  
B. Moreno ◽  
D. Appelhans ◽  
J. Sanchez-Nieves ◽  
...  
Keyword(s):  
Biophysical Characterization
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