A Mammalian Expression System for Rapid Production and Purification of Active MAP Kinase Phosphatases

2002 ◽  
Vol 24 (3) ◽  
pp. 481-488 ◽  
Author(s):  
Peili Chen ◽  
Dorothy Hutter ◽  
Pinghu Liu ◽  
Yusen Liu
Keyword(s):  
Map Kinase ◽  
Expression System ◽  
Mammalian Expression ◽  
Mammalian Expression System ◽  
Rapid Production

scholarly journals Glycosylation in a Mammalian Expression System Is Critical for the Production of Functionally Active Leukocyte Immunoglobulin-like Receptor A3 Protein

2013 ◽  
Vol 288 (46) ◽  
pp. 32873-32885 ◽  
Author(s):  
Terry H. Y. Lee ◽  
Ainslie Mitchell ◽  
Sydney Liu Lau ◽  
Hongyan An ◽  
Poornima Rajeaskariah ◽  
...  
Keyword(s):  
Expression System ◽  
Mammalian Expression ◽  
Mammalian Expression System

Functional Reconstitution of the Human Epithelial Na+ Channel in a Mammalian Expression System

Ion Channels ◽  
2006 ◽  
pp. 3-13
Author(s):  
Alexander Staruschenko ◽  
Rachell E. Booth ◽  
Oleh Pochynyuk ◽  
James D. Stockand ◽  
Qiusheng Tong
Keyword(s):  
Expression System ◽  
Na Channel ◽  
Mammalian Expression ◽  
Mammalian Expression System ◽  
Epithelial Na Channel

Functional Reconstitution of the Human Epithelial Na+ Channel in a Mammalian Expression System

Ion Channels ◽  
2006 ◽  
pp. 1-114
Author(s):  
Alexander Staruschenko ◽  
Rachell E. Booth ◽  
Oleh Pochynyuk ◽  
James D. Stockand ◽  
Qiusheng Tong
Keyword(s):  
Expression System ◽  
Mammalian Expression ◽  
Mammalian Expression System

A NOVEL MAMMALIAN EXPRESSION SYSTEM BASED ON THE GLOBIN LOCUS CONTROL REGION

1992 ◽  
pp. 68-70
Author(s):  
J McLean ◽  
M Hollis ◽  
M Needham ◽  
C Gooding ◽  
K Hudson ◽  
...  
Keyword(s):  
Control Region ◽  
Expression System ◽  
Locus Control Region ◽  
Mammalian Expression ◽  
Mammalian Expression System

Production of Recombinant Human CSF–1 in an Inducible Mammalian Expression System

1988 ◽  
Vol 6 (3) ◽  
pp. 287-290 ◽  
Author(s):  
John F. Weaver ◽  
Frank McCormick ◽  
M. Michele Manos
Keyword(s):  
Expression System ◽  
Mammalian Expression ◽  
Mammalian Expression System

scholarly journals High Expression and Purification of Amino-Terminal Fragment of Human Amyloid Precursor Protein inPichia pastorisand Partial Analysis of Its Properties

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Wei Li ◽  
Xiang Gao ◽  
Junle Ren ◽  
Ting An ◽  
Yan Liu
Keyword(s):  
Amyloid Precursor Protein ◽  
Expression System ◽  
Precursor Protein ◽  
High Yield ◽  
Human Neuroblastoma ◽  
Amino Terminal ◽  
Mammalian Expression ◽  
Terminal Fragment ◽  
Mammalian Expression System ◽  
Amino Terminal Fragment

The cleaved amino-terminal fragment of human amyloid precursor protein (N-APP) binds death receptor 6 (DR6) and triggers a caspase-dependent self-destruction process, which was suggested to contribute to Alzheimer’s disease. To investigate the N-APP-DR6-induced degeneration pathway at the molecular level, obtaining abundant and purified N-APP is fundamental and critical. The recombinant N-APP has been produced in mammalian expression system. However, the cost and yield disadvantages of mammalian expression system make it less ideal for protein mass production. Here, we successfully expressed and purified recombinant N-terminal 18-285 amino acid residues of human amyloid precursor protein from the methylotrophic yeastPichia pastoriswith a high yield of 50 mg/L. Flow cytometry indicated the purified N-APP-induced obvious apoptosis of human neuroblastoma SHEP cells.

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