Effect of Cadmium on Osteogenesis within Diffusion Chambers by Bone Marrow Cells: Biochemical Evidence of Decreased Bone Formation Capacity

After heterotopic (e.g. subcutaneous) transplantation of bone marrow, haemopoiesis in the graft ceases; reticular tissue develops instead, and later bone is formed (Denis, 1958). The result can be achieved by grafting either free pieces of bone marrow or those placed in diffusion chambers (Petrakova, Tolmacheva & Friedenstein, 1963; Rosin, Freiberg & Sajnek, 1963). In the case of free transplantation the bone formed is later filled with bone marrow. After transplantation in diffusion chambers haemopoiesis does not recur despite the development of a considerable mass of bone in the chambers (Friedenstein, 1965). The population of bone marrow cells is very heterogeneous, including haemopoietic cells, reticular cells and endosteum elements. According to generally accepted views this population is a mixture of individual cell lines capable of mutual transformations within certain limits (Maximov, 1927; Burwell, 1964). After transplantation some of the pathways of differentiation open to bone marrow tissue (formation of reticular and bone tissues) are stimulated, while others (haemopoiesis) are arrested.

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Osteoclastogenesis from bone marrow cells during estrogen-induced medullary bone formation in Japanese quails

Journal of Molecular Histology ◽

10.1007/s10735-019-09835-x ◽

2019 ◽

Vol 50 (4) ◽

pp. 389-404 ◽

Cited By ~ 2

Author(s):

Shinji Hiyama ◽

Miyuki Yokoi ◽

Yuichi Akagi ◽

Yumiko Kadoyama ◽

Kiichi Nakamori ◽

...

Keyword(s):

Bone Marrow ◽

Bone Formation ◽

Bone Marrow Cells ◽

Medullary Bone ◽

Japanese Quails ◽

Marrow Cells

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Proliferation of Human Bone Marrow Cells in Diffusion Chambers Implanted Into Normal or Irradiated Mice

Blood ◽

10.1182/blood.v40.2.163.163 ◽

1972 ◽

Vol 40 (2) ◽

pp. 163-173 ◽

Cited By ~ 62

Author(s):

Arne Boyum ◽

Werner Boecker ◽

Arland L. Carsten ◽

Eugene P. Cronkite

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Bone Marrow Cells ◽

Human Bone ◽

Human Bone Marrow ◽

Culture Period ◽

Diffusion Chambers ◽

Significant Difference ◽

Lymphocyte Number ◽

Marrow Cells

Abstract Diffusion chambers containing normal, human bone marrow cells were implanted in the abdominal cavity of normal and irradiated mice. Granulocytic cells and macrophages proliferated in the chambers. The number of cells in the granulocytic series recovered from the chambers dropped to 60% after 1 day; during the next 7 days it varied between 40% and 60% of the inoculated number of granulocytes, with no difference between irradiated and non-irradiated animals. From day 9 the yield of cells in granulocytic series increased in chambers from irradiated animals, and a higher percentage of cells were in the proliferating pool of the granulocytic series. Simultaneously, the cell yield in chambers from normal animals dropped markedly and consisted mostly of mature granulocytes. In both groups the percentage of eosinophilic cells increased significantly during the last part of the culture period. The enhanced growth in the irradiated mice suggests an increased self-renewal of granulocytic stem cells, leading to a larger yield of differentiated granulocytic cells later in the culture period. A shortened generation time and/or increased cloning efficiency of stem cells may also contribute to the enhanced granulocyte production. The suppression of the immune reactivity by irradiation of the host animals may allow better proliferation by delaying production of cytotoxic antibodies against the xenogenic human cells. The number of macrophages increased gradually, and there was no significant difference between irradiated and nonirradiated animals. The lymphocyte number decreased after implantation and varied between 30% and 50% of the inoculated number. From day 11, the lymphocyte number dropped more in normal animals than in irradiated animals.

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Enhancement of human muscle growth in diffusion chambers by bone marrow cells

Virchows Archiv B Cell Pathology Including Molecular Pathology ◽

10.1007/bf02890279 ◽

1982 ◽

Vol 41 (1) ◽

pp. 171-180 ◽

Cited By ~ 5

Author(s):

R. Yarom ◽

S. Meyer ◽

O. Carmy ◽

B. Ghidoni ◽

R. More

Keyword(s):

Bone Marrow ◽

Bone Marrow Cells ◽

Muscle Growth ◽

Human Muscle ◽

Diffusion Chambers ◽

Marrow Cells

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Human bone marrow cells synthesize collagen, in diffusion chambers, implanted into the normal rat

Cell Biology International Reports ◽

10.1016/0309-1651(87)90113-5 ◽

1987 ◽

Vol 11 (2) ◽

pp. 125-130 ◽

Cited By ~ 6

Author(s):

J DAVIES

Keyword(s):

Bone Marrow ◽

Bone Marrow Cells ◽

Human Bone ◽

Human Bone Marrow ◽

Diffusion Chambers ◽

Normal Rat ◽

Marrow Cells

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STIMULATION OF BONE MARROW CELLS AND BONE FORMATION BY NACRE. IN VIVO AND IN VITRO STUDIES

Bioceramics ◽

10.1142/9789814291064_0073 ◽

1999 ◽

Author(s):

M. Lamghari ◽

S. Berland ◽

A. Laurent ◽

H. Huet ◽

M.J. Almeida ◽

...

Keyword(s):

Bone Marrow ◽

Bone Formation ◽

Bone Marrow Cells ◽

In Vitro Studies ◽

Stimulation Of ◽

Marrow Cells

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The Cultivation of Mouse Bone Marrow in Vivo

Blood ◽

10.1182/blood.v14.9.1040.1040 ◽

1959 ◽

Vol 14 (9) ◽

pp. 1040-1046 ◽

Cited By ~ 31

Author(s):

IRWIN BERMAN ◽

HENRY S. KAPLAN

Keyword(s):

Bone Marrow ◽

Peritoneal Cavity ◽

Normal Mouse ◽

Bone Marrow Cells ◽

Mouse Bone Marrow ◽

Considerable Time ◽

Diffusion Chambers ◽

Mouse Bone Marrow Cells ◽

Marrow Cells

Abstract The cultivation of normal mouse bone marrow cells in diffusion chambers implanted into the peritoneal cavity of mice has been described. Mouse bone marrow cells cultivated by this method continue to undergo differentiation and maintain their morphologic identity for a considerable time.

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