<em>Klebsiella pneumoniae</em> is a major cause of nosocomial pneumonia, septicemia and urinary tract infections, especially in newborns, blood cancer patients, and other immunocompromised candidates. The control of <em>K. pneumoniae</em> is a complicated issue due to its tight pathogenesis. Immuno-prophylactic preparations, especially those directed toward the <em>bacterium</em> O-antigen, showed to be the most successful way to prevent the infection incidence. However, all previously proposed preparations were either of limited spectrum or non-maternal, and hence not targeting the main <em>Klebsiella</em> patients. Moreover, all preparations were directed only to prevent the respiratory diseases due to that pathogen. This article addresses the development of a method originally used to purify the non-capsular bacterial- endotoxins, as a new and easy method for vaccine production against <em>K. pneumoniae</em>. The application of this method was preceded by a biotechnological control of capsular polysaccharide production in <em>K. pneumoniae</em>. The new produced natural conjugate between the bacterial O-antigen and its outer membrane proteins was evaluated by physicochemical and immunological methods to investigate its purity, integrity, safety and immunogenicity. It showed to be pure, stable, safe for use, and able to elicit a protective immunoglobulin titer against different <em>Klebsiella</em> infections. This immune-response proved to be transferable to the offspring of the vaccinated experimental rabbits via placenta.
Auxin-binding protein from coleoptile membranes of corn (Zea mays L.). I. Purification by immunological methods and characterization.
