Recombination: Cytoplasmic male sterility and fertility restoration in higher plants

Abstract Background Cytoplasmic male sterility (CMS), which naturally exists in higher plants, is a useful mechanism for analyzing nuclear and mitochondrial genome functions and identifying the role of mitochondrial genes in the plant growth and development. Polima (pol) CMS is the most universally valued male sterility type in oil-seed rape. Previous studies have described the pol CMS restorer gene Rfp and the sterility-inducing gene orf224 in oil-seed rape, located in mitochondria. However, the mechanism of fertility restoration and infertility remains unknown. Moreover, it is still unknown how the fecundity restorer gene interferes with the sterility gene, provokes the sterility gene to lose its function, and leads to fertility restoration. Result In this study, we used multi-omics joint analysis to discover candidate genes that interact with the sterility gene orf224 and the restorer gene Rfp of pol CMS to provide theoretical support for the occurrence and restoration mechanisms of sterility. Via multi-omics analysis, we screened 24 differential genes encoding proteins related to RNA editing, respiratory electron transport chain, anther development, energy transport, tapetum development, and oxidative phosphorylation. Using a yeast two-hybrid assay, we obtained a total of seven Rfp interaction proteins, with orf224 protein covering five interaction proteins. Conclusions We propose that Rfp and its interacting protein cleave the transcript of atp6/orf224, causing the infertility gene to lose its function and restore fertility. When Rfp is not cleaved, orf224 poisons the tapetum cells and anther development-related proteins, resulting in pol CMS mitochondrial dysfunction and male infertility. The data from the joint analysis of multiple omics provided information on pol CMS’s potential molecular mechanism and will help breed B. napus hybrids.

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The Dynamics of Gynodioecy in Plantago lanceolatu L. II. Mode of Action and Frequencies of Restorer Alleles

Genetics ◽

10.1093/genetics/147.3.1317 ◽

1997 ◽

Vol 147 (3) ◽

pp. 1317-1328

Author(s):

Anita A de Haan ◽

Hans P Koelewijn ◽

Maria P J Hundscheid ◽

Jos M M Van Damme

Keyword(s):

Cytoplasmic Male Sterility ◽

Male Sterility ◽

Allele Frequency ◽

Mode Of Action ◽

Male Fertility ◽

Fertility Restoration ◽

Plantago Lanceolata ◽

Allele Frequencies ◽

Male Sterile ◽

Male Fertility Restoration

Male fertility in Plantago lanceolata is controlled by the interaction of cytoplasmic and nuclear genes. Different cytoplasmic male sterility (CMS) types can be either male sterile or hermaphrodite, depending on the presence of nuclear restorer alleles. In three CMS types of P. lanceolata (CMSI, CMSIIa, and CMSIIb) the number of loci involved in male fertility restoration was determined. In each CMS type, male fertility was restored by multiple genes with either dominant or recessive action and capable either of restoring male fertility independently or in interaction with each other (epistasis). Restorer allele frequencies for CMSI, CMSIIa and CMSIIb were determined by crossing hermaphrodites with “standard” male steriles. Segregation of male steriles vs. non-male steriles was used to estimate overall restorer allele frequency. The frequency of restorer alleles was different for the CMS types: restorer alleles for CMSI were less frequent than for CMSIIa and CMSIIb. On the basis of the frequencies of male steriles and the CMS types an “expected” restorer allele frequency could be calculated. The correlation between estimated and expected restorer allele frequency was significant.

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INHERITANCE OF FERTILITY RESTORATION INVOLVING WILD ABORTIVE CYTOPLASMIC MALE STERILITY SYSTEM IN RICE (Oryza sativa L.)

Bangladesh Journal of Plant Breeding and Genetics ◽

10.3329/bjpbg.v24i1.16997 ◽

2011 ◽

Vol 24 (1) ◽

pp. 33-40

Author(s):

M. J. Hasan ◽

M. U. Kulsum ◽

A. Ansari ◽

A. K. Paul ◽

P. L. Biswas

Keyword(s):

Oryza Sativa ◽

Cytoplasmic Male Sterility ◽

Male Sterility ◽

Gene Action ◽

Oryza Sativa L ◽

Fertility Restoration ◽

Dominant Gene ◽

Recessive Gene ◽

Male Sterile ◽

Male Sterile Line

Inheritance of fertility restoration was studied in crosses involving ten elite restorer lines of rice viz. BR6839-41-5-1R, BR7013-62-1-1R, BR7011-37-1-2R, BR10R, BR11R, BR12R, BR13R, BR14R, BR15R and BR16R and one male sterile line Jin23A with WA sources of cytoplasmic male sterility. The segregation pattern for pollen fertility of F2 and BC1 populations of crosses involving Jin23A indicated the presence of two independent dominant fertility restoring genes. The mode of action of the two genes varied in different crosses revealing three types of interaction, i.e. epistasis with dominant gene action, epistasis with recessive gene action, and epistasis with incomplete dominance.DOI: http://dx.doi.org/10.3329/bjpbg.v24i1.16997

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Inheritance of Fertility Restoration for Cytoplasmic Male Sterility in a New Gossypium barbadense Restorer

Agricultural Sciences in China ◽

10.1016/s1671-2927(09)60119-x ◽

2010 ◽

Vol 9 (4) ◽

pp. 472-476 ◽

Cited By ~ 1

Author(s):

Xiao-quan ZHANG ◽

Xue-de WANG ◽

Pei-dong JIANG ◽

Wei ZHU

Keyword(s):

Cytoplasmic Male Sterility ◽

Male Sterility ◽

Fertility Restoration ◽

Gossypium Barbadense

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Accelerated molecular breeding of novel cytoplasmic male sterility lines in rice using orfH79 or orf290 haplotypes

10.21203/rs.3.rs-19752/v1 ◽

2020 ◽

Author(s):

Yanping Tan ◽

Tong Chen ◽

Ze Tian ◽

Jiayang Li ◽

Xuequn Liu ◽

...

Keyword(s):

Cytoplasmic Male Sterility ◽

Male Sterility ◽

Higher Plants ◽

Amino Acid Sequences ◽

Cultivated Rice ◽

Maintainer Line ◽

Breeding Programs ◽

Aa Genome ◽

Allele Variation ◽

Grain Security

Abstract The identification and development of new cytoplasmic male sterility (CMS) lines in higher plants is important for the preservation of grain security and the prevention of homogenization of hybrid rice. Molecular markers assisted selection (MAS) based on CMS-associated genes or mitochondrial-specific chimeric sequences are important for rapid and effective breeding of new CMS lines and hybrids. In our study, the distribution and allele variation of orfH79 and orf290 genes were characterized from 273 wild and cultivated rice in the AA genome species. Based on the alignment of nucleotide and amino acid sequences, four accessions with orfH79 and three accessions with orf290 were screened. Four novel CMS lines carrying orfH79 haplotypes and three novel CMS lines carrying orf290 haplotypes were then developed using multiple backcross generations with a maintainer line under MAS. The breeding process used in our study provides an efficient and feasible approach for selecting new CMS lines. CMS lines selected in our study are important for enriching rice germplasm resources and guaranteeing rice breeding programs.

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