BASIC NUTRIENT CONSUMPTION AND REMOVAL WITH RAPESEED SEEDS WHEN APPLYING DIFFERENT FERTILIZER COMBINATIONS

In the context of different fertilizer combination applica-tion (urea-ammonia liquor KAS-32, liquid complex fertilizer ZhKU, and compound NPK fertilizerDiammophoska) N89P26K26S14, N70P26K26S14, N73P39K13S14, N89P39K13S14, N87P37S14and N71P37S14, we revealed increased removal of CaO to 15.6-20 kg ha as opposed to 13.8 kg ha in the con-trol; MgO to 12.3-16 kg ha compared to 10.2 kg ha in the control. Taking into account Ca and Mg removal without return, it requires the introduction of compound fertilizers containing these nutrients into the fertilizer system. Nitro-gen removal was 110.3-165.5 kg ha compared to 102.6 kg ha, and sulfur -10.9-12.8 kg ha compared to 7.8 kg ha in the control. The S/N ratio ranged from 8.9-15.4 compared to 14 in the control. For the most part, it was equal to 10.7-12.9 which was indicative of satisfactory sulfur supply to oil seed rape crops and the need to increase sulfur dose to 21-28 kg ha. The largest removal of all nutrients was found inthe variants with N73P39K13S14and N71P37S14; the highest yield was formed with N73P39K13S14with N:P:K removal ratio of 1:0.39:0.21

High-generation near-isogenic lines combined with multi-omics to study the mechanism of polima cytoplasmic male sterility

Background Cytoplasmic male sterility (CMS), which naturally exists in higher plants, is a useful mechanism for analyzing nuclear and mitochondrial genome functions and identifying the role of mitochondrial genes in the plant growth and development. Polima (pol) CMS is the most universally valued male sterility type in oil-seed rape. Previous studies have described the pol CMS restorer gene Rfp and the sterility-inducing gene orf224 in oil-seed rape, located in mitochondria. However, the mechanism of fertility restoration and infertility remains unknown. Moreover, it is still unknown how the fecundity restorer gene interferes with the sterility gene, provokes the sterility gene to lose its function, and leads to fertility restoration. Result In this study, we used multi-omics joint analysis to discover candidate genes that interact with the sterility gene orf224 and the restorer gene Rfp of pol CMS to provide theoretical support for the occurrence and restoration mechanisms of sterility. Via multi-omics analysis, we screened 24 differential genes encoding proteins related to RNA editing, respiratory electron transport chain, anther development, energy transport, tapetum development, and oxidative phosphorylation. Using a yeast two-hybrid assay, we obtained a total of seven Rfp interaction proteins, with orf224 protein covering five interaction proteins. Conclusions We propose that Rfp and its interacting protein cleave the transcript of atp6/orf224, causing the infertility gene to lose its function and restore fertility. When Rfp is not cleaved, orf224 poisons the tapetum cells and anther development-related proteins, resulting in pol CMS mitochondrial dysfunction and male infertility. The data from the joint analysis of multiple omics provided information on pol CMS’s potential molecular mechanism and will help breed B. napus hybrids.

Unwrapping the Spontaneous Flora: On the Appropriation of Weed Labour

Part of the raw material accumulation for the medicinal plant industry in Romania is reliant on gathering plants from the so-called spontaneous flora. The imagery of medicinal plants played upon by medicinal plant product manufacturers is often abundant in visions of either wilderness or traditional peasant landscapes such as pastures. This article aims to present instead two different spaces where medicinal plants come from: wild pansy from within an oil seed rape cultivation, and elderflowers and nettles from the ruins of a former socialist orchard. These spaces of spontaneous flora highlight the process of capital’s appropriation or salvage of the ‘free’ reproductive labour (spontaneous growth) of weeds often at odds and against other capitalist processes. Moreover, salvaging or scrounging is done through the cheap labour of a family whose livelihood depends on work both inside and outside of this capitalist process. These places, therefore, highlight the tension between the spontaneous flora and scroungers on the ground and Nature with its ancestral peasants on the supermarket and nature shop shelves.

Biomass Yield of Different Plants for Biogass Production

In order to investigate yield potential of plants probably suitable for biogas production preliminary field trials were carried out at Research and Study farm “Vecauce” in 2010 using eight annual plant species: maize, winter oil-seed rape, oil radish, sunflower, foxtail millet, millet, hemp and amaranth. All species (except oil radish) were represented with several varieties, and some species were harvested at 2-3 development stages. Obtained fresh biomass yield was from 33.05 (millet „Rudes‟) till 74.60 (amaranth „Raudonukai‟) t ha-1, but dry matter yield – from 6.98 (amaranth „Margiai‟) till 22.05 (winter oil-seed rape „Excalibur‟ at GS 85) t ha-1. It was self-evident that biomass yield substantially depended on used species, but also variety influence within the species was relevant. Our data clearly demonstrated substantial (p less then 0.05) harvest time influence by harvesting winter oil-seed rape and maize at three different times; the highest yield was obtained for rape at GS 85, but for maize – in early October. Studies are only at the initial stage and have to be continued.

The role and impact of N-Lock (N-stabilizer) to the utilization of N in the main arable crops

The nitrogen stabilizer called N-Lock can be used primarily with solid and liquid urea, UAN and other liquid nitrogen, slurry and manure. In corn it can be applied incorporated before sowing or with row-cultivator or applied with postemergent timing in tank-mix. In postemergent timing need precipitation for long effect. In oil seed rape and autumn cereals the N-Lock should be applied with liquid nitrogen in tank mix late winter or early spring (February-March). The dose rate is 2.5 l/ha. N-Lock increases the yield of maize, winter oil seed rape, winter wheat and winter barley 5-20 %. The yield increasing can be given the thousand grain weight. In case of high doses of nitrogen it can be observed higher yield. The quality parameter also improved, especially the oil content of winter oil seed rape and protein and gluten contents of winter wheat. The use of N-Lock increases the nitrogen retention of soil and reduces nitrate leaching towards the groundwater and the greenhouse effect gas emissions into the atmosphere. The degradation of the applied nitrogen is slowing down and the plant can uptake more nitrogen in long period. The effect of N-Lock the nitrogen is located in the upper soil layer of 0-30 cm and increasing the ammonium nitrogen form. The product can be mixed with herbicide products in main arable crops.