Metabolic Engineering of Microalgae for Biofuel Production

2019 ◽  
pp. 153-172 ◽  
Author(s):  
Mohammad Pooya Naghshbandi ◽  
Meisam Tabatabaei ◽  
Mortaza Aghbashlo ◽  
Muhammad Nauman Aftab ◽  
Irfana Iqbal
Keyword(s):  
Metabolic Engineering ◽  
Biofuel Production

Metabolic engineering ofEscherichia colifor biofuel production

Biofuels ◽  
2010 ◽  
Vol 1 (3) ◽  
pp. 493-504 ◽  
Author(s):  
Peng Xu ◽  
Mattheos AG Koffas
Keyword(s):  
Metabolic Engineering ◽  
Biofuel Production

Recent Progress on Microbial Metabolic Engineering for the Conversion of Lignocellulose Waste for Biofuel Production

2013 ◽  
pp. 119-145
Author(s):  
Shubhangini Sharma ◽  
Reena ◽  
Anil Kumar ◽  
Pallavi Mittal
Keyword(s):  
Metabolic Engineering ◽  
Recent Progress ◽  
Biofuel Production

Preliminary investigation of metabolic engineering in a novel host bacterium Corynebacterium crenatum for alcohol biofuel production

RSC Advances ◽  
2014 ◽  
Vol 4 (110) ◽  
pp. 65021-65030 ◽  
Author(s):  
Haifeng Su ◽  
Qiuli Lu ◽  
Yun Zhao ◽  
Juan Jiang ◽  
Zhao Zhao ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Preliminary Investigation ◽  
Biofuel Production ◽  
Host Bacterium ◽  
Corynebacterium Crenatum ◽  
Novel Host

Metabolic engineering of microorganisms for biofuel production

2018 ◽  
Vol 82 ◽  
pp. 3863-3885 ◽  
Author(s):  
Parastoo Majidian ◽  
Meisam Tabatabaei ◽  
Mehrshad Zeinolabedini ◽  
Mohammad Pooya Naghshbandi ◽  
Yusuf Chisti
Keyword(s):  
Metabolic Engineering ◽  
Biofuel Production

Biofuel production in Escherichia coli: the role of metabolic engineering and synthetic biology

2010 ◽  
Vol 86 (2) ◽  
pp. 419-434 ◽  
Author(s):  
James M. Clomburg ◽  
Ramon Gonzalez
Keyword(s):  
Escherichia Coli ◽  
Metabolic Engineering ◽  
Synthetic Biology ◽  
Biofuel Production

scholarly journals Improved Acetic Acid Resistance in Saccharomyces cerevisiae by Overexpression of theWHI2Gene Identified through Inverse Metabolic Engineering

2016 ◽  
Vol 82 (7) ◽  
pp. 2156-2166 ◽  
Author(s):  
Yingying Chen ◽  
Lisa Stabryla ◽  
Na Wei
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Acetic Acid ◽  
Metabolic Engineering ◽  
Acid Stress ◽  
Acid Resistance ◽  
Biofuel Production ◽  
Gene Target ◽  
Content Type ◽  
Inverse Metabolic Engineering ◽  
Acetic Acid Resistance

ABSTRACTDevelopment of acetic acid-resistantSaccharomyces cerevisiaeis important for economically viable production of biofuels from lignocellulosic biomass, but the goal remains a critical challenge due to limited information on effective genetic perturbation targets for improving acetic acid resistance in the yeast. This study employed a genomic-library-based inverse metabolic engineering approach to successfully identify a novel gene target,WHI2(encoding a cytoplasmatic globular scaffold protein), which elicited improved acetic acid resistance inS. cerevisiae. Overexpression ofWHI2significantly improved glucose and/or xylose fermentation under acetic acid stress in engineered yeast. TheWHI2-overexpressing strain had 5-times-higher specific ethanol productivity than the control in glucose fermentation with acetic acid. Analysis of the expression ofWHI2gene products (including protein and transcript) determined that acetic acid induced endogenous expression of Whi2 inS. cerevisiae. Meanwhile, thewhi2Δ mutant strain had substantially higher susceptibility to acetic acid than the wild type, suggesting the important role of Whi2 in the acetic acid response inS. cerevisiae. Additionally, overexpression ofWHI2and of a cognate phosphatase gene,PSR1, had a synergistic effect in improving acetic acid resistance, suggesting that Whi2 might function in combination with Psr1 to elicit the acetic acid resistance mechanism. These results improve our understanding of the yeast response to acetic acid stress and provide a new strategy to breed acetic acid-resistant yeast strains for renewable biofuel production.

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