Site-specific Localization of D-Amino Acids in Bioactive Peptides by Ion Mobility Spectrometry

2016 ◽  
pp. 43-53 ◽  
Author(s):  
Chenxi Jia ◽  
Christopher B. Lietz ◽  
Qing Yu ◽  
Lingjun Li
Keyword(s):  
Amino Acids ◽  
Ion Mobility Spectrometry ◽  
Ion Mobility ◽  
Bioactive Peptides ◽  
Site Specific ◽  
Specific Localization

scholarly journals Site-Specific Characterization of d-Amino Acid Containing Peptide Epimers by Ion Mobility Spectrometry

2014 ◽  
Vol 86 (6) ◽  
pp. 2972-2981 ◽  
Author(s):  
Chenxi Jia ◽  
Christopher B. Lietz ◽  
Qing Yu ◽  
Lingjun Li
Keyword(s):  
Amino Acid ◽  
Ion Mobility Spectrometry ◽  
Ion Mobility ◽  
Site Specific

scholarly journals Evaluation and Optimization of High-Field Asymmetric Waveform Ion-Mobility Spectrometry for Multiplexed Quantitative Site-Specific N-Glycoproteomics

2021 ◽  
Author(s):  
Pan Fang ◽  
Yanlong Ji ◽  
Ivan Silbern ◽  
Rosa Viner ◽  
Thomas Oellerich ◽  
...  
Keyword(s):  
Ion Mobility Spectrometry ◽  
Ion Mobility ◽  
High Field ◽  
Asymmetric Waveform ◽  
Site Specific

scholarly journals Evaluation and Optimization of High-Field Asymmetric Waveform Ion Mobility Spectrometry for Multiplexed Quantitative Site-specific N-glycoproteomics

2021 ◽  
Author(s):  
Pan Fang ◽  
Yanlong Ji ◽  
Ivan Silbern ◽  
Rosa Viner ◽  
Thomas Oellerich ◽  
...  
Keyword(s):  
Ion Mobility Spectrometry ◽  
Ion Mobility ◽  
High Field ◽  
Peptide Sequence ◽  
Asymmetric Waveform ◽  
Site Specific ◽  
Tandem Mass Tag ◽  
Quantitative Accuracy ◽  
Human Lymphoma ◽  
Different Characteristics

The heterogeneity and complexity of glycosylation hinder the depth of site-specific glycoproteomics analysis. High-field asymmetric-waveform ion-mobility spectrometry (FAIMS) has shown to improve the scope of bottom-up proteomics. The benefits of FAIMS for quantitative N glycoproteomics have not been investigated yet. In this work, we optimized FAIMS settings for N-glycopeptide identification, with or without the tandem mass tag (TMT) label. The optimized FAIMS approach significantly increased the identification of site-specific N glycopeptides derived from the purified IgM protein or human lymphoma cells. We explored in detail the changes in FAIMS mobility caused by N glycopeptides with different characteristics, including TMT labeling, charge state, glycan type, peptide sequence, glycan size and precursor m/z. Importantly, FAIMS also improved multiplexed N glycopeptide quantification, both with the standard MS2 acquisition method and with our recently developed Glyco-SPS-MS3 method. The combination of FAIMS and Glyco-SPS-MS3 provided the highest quantitative accuracy and precision. Our results demonstrate the advantages of FAIMS for improved mass-spectrometry-based qualitative and quantitative N glycoproteomics.

Electrospray Ionization High-Resolution Ion Mobility Spectrometry for the Detection of Organic Compounds, 1. Amino Acids

2001 ◽  
Vol 73 (13) ◽  
pp. 3028-3034 ◽  
Author(s):  
Luther W. Beegle ◽  
Isik Kanik ◽  
Laura Matz ◽  
Herbert H. Hill
Keyword(s):  
Amino Acids ◽  
High Resolution ◽  
Electrospray Ionization ◽  
Organic Compounds ◽  
Ion Mobility Spectrometry ◽  
Ion Mobility

Electrospray Ionization Ion Mobility Spectrometry of Amino Acids:  Ion Mobilities and a Mass−Mobility Correlation

2004 ◽  
Vol 108 (27) ◽  
pp. 5785-5792 ◽  
Author(s):  
Paul V. Johnson ◽  
Hugh I. Kim ◽  
Luther W. Beegle ◽  
Isik Kanik
Keyword(s):  
Amino Acids ◽  
Electrospray Ionization ◽  
Ion Mobility Spectrometry ◽  
Ion Mobility

Effective Chiral Discrimination of Amino Acids through Oligosaccharide Incorporation by Trapped Ion Mobility Spectrometry

2020 ◽  
Author(s):  
Chengyi Xie ◽  
Liancheng Gu ◽  
Qidi Wu ◽  
Lei Li ◽  
Chenlu Wang ◽  
...  
Keyword(s):  
Amino Acids ◽  
Ion Mobility Spectrometry ◽  
Ion Mobility ◽  
Chiral Discrimination ◽  
Trapped Ion ◽  
Trapped Ion Mobility Spectrometry

Separation of amino acids by ion mobility spectrometry

2000 ◽  
Vol 902 (2) ◽  
pp. 433-437 ◽  
Author(s):  
G.Reid Asbury ◽  
Herbert H Hill
Keyword(s):  
Amino Acids ◽  
Ion Mobility Spectrometry ◽  
Ion Mobility

scholarly journals Site-Specific Mapping of Sialic Acid Linkage Isomers by Ion Mobility Spectrometry

2016 ◽  
Vol 88 (10) ◽  
pp. 5212-5217 ◽  
Author(s):  
Miklos Guttman ◽  
Kelly K. Lee
Keyword(s):  
Sialic Acid ◽  
Ion Mobility Spectrometry ◽  
Ion Mobility ◽  
Site Specific ◽  
Linkage Isomers ◽  
Specific Mapping ◽  
Sialic Acid Linkage

Enantiomer Separation of Amino Acids by Complexation with Chiral Reference Compounds and High-Field Asymmetric Waveform Ion Mobility Spectrometry:  Preliminary Results and Possible Limitations

2007 ◽  
Vol 79 (7) ◽  
pp. 2850-2858 ◽  
Author(s):  
Axel Mie ◽  
Magnus Jörntén-Karlsson ◽  
Bengt-Olof Axelsson ◽  
Andrew Ray ◽  
Curt T. Reimann
Keyword(s):  
Amino Acids ◽  
Ion Mobility Spectrometry ◽  
Ion Mobility ◽  
High Field ◽  
Enantiomer Separation ◽  
Asymmetric Waveform ◽  
Preliminary Results
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