Multi-Point Covalent Immobilization of Enzymes on Supports Activated with Epoxy Groups: Stabilization of Industrial Enzymes

2020 ◽  
pp. 109-117
Author(s):  
Cesar Mateo ◽  
Olga Abian ◽  
Gloria Fernandez-Lorente ◽  
Benevides C. C. Pessela ◽  
Valeria Grazu ◽  
...  
Keyword(s):  
Covalent Immobilization ◽  
Industrial Enzymes ◽  
Immobilization Of Enzymes ◽  
Epoxy Groups

Advances in the design of new epoxy supports for enzyme immobilization–stabilization

2007 ◽  
Vol 35 (6) ◽  
pp. 1593-1601 ◽  
Author(s):  
C. Mateo ◽  
V. Grazú ◽  
B.C.C. Pessela ◽  
T. Montes ◽  
J.M. Palomo ◽  
...  
Keyword(s):  
Physical Adsorption ◽  
Immobilized Enzymes ◽  
Covalent Immobilization ◽  
Covalent Attachment ◽  
Industrial Enzymes ◽  
Protein Surface ◽  
Strong Stabilization ◽  
Support Surfaces ◽  
Multipoint Covalent Attachment ◽  
Epoxy Groups

Multipoint covalent immobilization of enzymes (through very short spacer arms) on support surfaces promotes a very interesting ‘rigidification’ of protein molecules. In this case, the relative positions of each residue of the enzyme involved in the immobilization process have to be preserved unchanged during any conformational change induced on the immobilized enzyme by any distorting agent (heat, organic solvents etc.). In this way, multipoint covalent immobilization should induce a very strong stabilization of immobilized enzymes. Epoxy-activated supports are able to chemically react with all nucleophile groups placed on the protein surface: lysine, histidine, cysteine, tyrosine etc. Besides, epoxy groups are very stable. This allows the performance of very long enzyme–support reactions, enabling us to get very intense multipoint covalent attachment. In this way, these epoxy supports seem to be very suitable to stabilize industrial enzymes by multipoint covalent attachment. However, epoxy groups exhibit a low intermolecular reactivity towards nucleophiles and hence the enzymes are not able to directly react with the epoxy supports. Thus a rapid physical adsorption of enzymes on the supports becomes a first step, followed by an additional rapid ‘intramolecular’ reaction between the already adsorbed enzyme and the activated support. In this situation, a suitable first orientation of the enzyme on the support (e.g. through regions that are very rich in nucleophiles) is obviously necessary to get a very intense additional multipoint covalent immobilization. The preparation of different ‘generations’ of epoxy supports and the design of different protocols to fully control the first interaction between enzymes and epoxy supports will be reviewed in this paper. Finally, the possibilities of a directed immobilization of mutated enzymes (change of an amino acid by cysteine on specific points of the protein surface) on tailor-made disulfide-epoxy supports will be discussed as an almost-ideal procedure to achieve very intense and very efficient rigidification of a desired region of industrial enzymes.

scholarly journals A Flexible Method for the Stable, Covalent Immobilization of Enzymes at Electrode Surfaces

2017 ◽  
Vol 4 (6) ◽  
pp. 1528-1534 ◽  
Author(s):  
Firas A. Al-Lolage ◽  
Marta Meneghello ◽  
Su Ma ◽  
Roland Ludwig ◽  
Philip N. Bartlett
Keyword(s):  
Covalent Immobilization ◽  
Electrode Surfaces ◽  
Immobilization Of Enzymes

In situ synthesis of porous silicananoparticles for covalent immobilization of enzymes

Nanoscale ◽  
2012 ◽  
Vol 4 (2) ◽  
pp. 414-416 ◽  
Author(s):  
Xiaowei Yang ◽  
Zhengwei Cai ◽  
Zhangmei Ye ◽  
Sheng Chen ◽  
Yu Yang ◽  
...  
Keyword(s):  
Covalent Immobilization ◽  
In Situ Synthesis ◽  
Immobilization Of Enzymes

Functionalized bicomponent polymer membranes as supports for covalent immobilization of enzymes

2015 ◽  
Vol 96 ◽  
pp. 5-13 ◽  
Author(s):  
Teodor Sandu ◽  
Andrei Sarbu ◽  
Celina Maria Damian ◽  
Delia Patroi ◽  
Tanta Verona Iordache ◽  
...  
Keyword(s):  
Polymer Membranes ◽  
Covalent Immobilization ◽  
Immobilization Of Enzymes

Large scale applications of immobilized enzymes call for sustainable and inexpensive solutions: rice husks as renewable alternatives to fossil-based organic resins

RSC Advances ◽  
2016 ◽  
Vol 6 (68) ◽  
pp. 63256-63270 ◽  
Author(s):  
L. Corici ◽  
V. Ferrario ◽  
A. Pellis ◽  
C. Ebert ◽  
S. Lotteria ◽  
...  
Keyword(s):  
Rice Husk ◽  
Large Scale ◽  
Immobilized Enzymes ◽  
Covalent Immobilization ◽  
Rice Husks ◽  
Immobilization Of Enzymes

Rice husk for physical and covalent immobilization of enzymes: a sustainable and economic alternative to fossil-based organic resins.

scholarly journals Microwave‐assisted covalent immobilization of enzymes on inorganic surfaces

2014 ◽  
Vol 14 (5) ◽  
pp. 493-499 ◽  
Author(s):  
Regina Plagemann ◽  
Jan Langermann ◽  
Udo Kragl
Keyword(s):  
Covalent Immobilization ◽  
Microwave Assisted ◽  
Immobilization Of Enzymes
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