simultaneous saccharification
Recently Published Documents





2022 ◽  
Vol 12 (1) ◽  
Antonio D. Moreno ◽  
Cristina González-Fernández ◽  
Elia Tomás-Pejó

AbstractIncreasing yeast robustness against lignocellulosic-derived inhibitors and insoluble solids in bioethanol production is essential for the transition to a bio-based economy. This work evaluates the effect exerted by insoluble solids on yeast tolerance to inhibitory compounds, which is crucial in high gravity processes. Adaptive laboratory evolution (ALE) was applied on a xylose-fermenting Saccharomyces cerevisiae strain to simultaneously increase the tolerance to lignocellulosic inhibitors and insoluble solids. The evolved strain gave rise to a fivefold increase in bioethanol yield in fermentation experiments with high concentration of inhibitors and 10% (w/v) of water insoluble solids. This strain also produced 5% (P > 0.01) more ethanol than the parental in simultaneous saccharification and fermentation of steam-exploded wheat straw, mainly due to an increased xylose consumption. In response to the stress conditions (solids and inhibitors) imposed in ALE, cells induced the expression of genes related to cell wall integrity (SRL1, CWP2, WSC2 and WSC4) and general stress response (e.g., CDC5, DUN1, CTT1, GRE1), simultaneously repressing genes related to protein synthesis and iron transport and homeostasis (e.g., FTR1, ARN1, FRE1), ultimately leading to the improved phenotype. These results contribute towards understanding molecular mechanisms that cells might use to convert lignocellulosic substrates effectively.

2022 ◽  
Vol 182 ◽  
pp. 274-284
Ederson Paulo Xavier Guilherme ◽  
Leticia Maria Zanphorlin ◽  
Amanda Silva Sousa ◽  
Renan Yuji Miyamoto ◽  
Carlos Giovani Oliveira Bruziquesi ◽  

YMER Digital ◽  
2021 ◽  
Vol 20 (12) ◽  
pp. 819-833
Mrudhula Reddivari ◽  
P Dinesh Sankar Reddy ◽  

Looking at the environmental hazards being posed by indiscriminate use of synthetic plastics, abundant research is being done to explore various bio-degradable polymers. In the present study, Cellulose Nano Fibers (CNFs) were extracted from Pineapple Crown using mechano chemical treatment, PLA was synthesized by Simultaneous Saccharification and Fermentation using cellulase enzyme on Acacia Arabica as substrate. Further, ZnO nanoparticles were synthesized by using different precursors. The biocomposite sheets of PLA, PLA+ 5%-20% CNFs, PLA+5% ZnO+5-20 % CNFs and PLA+10% ZnO+5-20 % CNFs were solvent casted. Microbial efficacy test was done using E.coli and with inclusion of ZnO nanoparticles the microbial resistance has increased. Noteworthy vibration band of the sheets were observed in the wavelength range of 3700 to 2800 cm-1 from the FTIR analysis, which shows that there is only a physical interaction rather than chemical. The crystallinity increased for initial concentration, but was similar to the neat PLA. Significant increase in tensile strength and maximum elongation at break was observed in PLA + 5% ZnO + 10% CNFs sheet. Sheets were allowed to degrade naturally and significant weight loss was observed after 120 days with maximum reduction of 38.4 %. Morphological analysis through SEM revealed the uniform distribution of fillers in the polymer matrix. TGA studies have shown that the degradation temperatures were in the range of 320-405oC. The thermal stability decreased with the increase in ZnO concentration. The results have shown a promising and sustainable use in various applications in view of microbial resistance and bio-degradability.

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Agustin Krisna Wardani ◽  
Aji Sutrisno ◽  
Titik Nur Faida ◽  
Retno Dwi Yustina ◽  
Untung Murdiyatmo

Background. Oil palm trunk (OPT) with highly cellulose content is a valuable bioresource for bioethanol production. To produce ethanol from biomass, pretreatment is an essential step in the conversion of lignocellulosic biomass to fermentable sugars such as glucose and xylose. Several pretreatment methods have been developed to overcome biomass recalcitrance. In this study, the effects of different pretreatment methods such as alkali pretreatment, microwave-alkali, and alkaline peroxide combined with autoclave on the lignocellulosic biomass structure were investigated. Moreover, ethanol production from the treated biomass was performed by simultaneous saccharification and cofermentation (SSCF) under different temperatures, fermentation times, and cell ratios of Saccharomyces cerevisiae NCYC 479 and pentose-utilizing yeast, Pichia stipitis NCYC 1541. Results. Pretreatment resulted in a significant lignin removal up to 83.26% and cellulose released up to 80.74% in treated OPT by alkaline peroxide combined with autoclave method. Enzymatic hydrolysis of treated OPT resulted in an increase in fermentable sugar up to 93.22%. Optimization of SSCF by response surface method showed that the coculture could work together to produce maximum ethanol (1.89%) and fermentation efficiency (66.14%) under the optimized condition. Conclusion. Pretreatment by alkaline peroxide combined with autoclave method and SSCF process could be expected as a promising system for ethanol production from oil palm trunk and various lignocellulosic biomass.

Fermentation ◽  
2021 ◽  
Vol 7 (4) ◽  
pp. 310
Nasib Qureshi ◽  
Badal Saha ◽  
Siqing Liu ◽  
Thaddeus Ezeji ◽  
Nancy Nichols

Butanol was produced commercially from cornstarch and sugarcane molasses (renewable resources) until 1983, when production of these plants was forced to cease because of unfavorable economics of production caused in part by escalating prices of these feedstocks. During recent years, the focus of research has been on the use of economically available agricultural biomass and residues and cutting-edge science and technology to make butanol production a commercially viable process again. In this study, we produced butanol from sweet sorghum bagasse (SSB) by employing high concentrations of SSB solids and integrated process technology through which simultaneous saccharification, fermentation, and recovery (SSFR) were conducted as one unit operation. The concentrated SSB (16–22% dry wt. basis or 160–220 gL−1) was used to reduce reactor size and potentially reduce fixed and operational costs. Indeed, ABE productivity and yield of 0.21 gL−1h−1 and 0.39 were obtained, respectively, when 160 gL−1 SSB (16%, dry wt.) was used in the SSFR process. In nonintegrated systems, use of >90 gL−1 solid loading is improbable and has not been done until this study.

2021 ◽  
Vol 7 (12) ◽  
pp. 1038
Hao Ji ◽  
Ke Xu ◽  
Xiameng Dong ◽  
Da Sun ◽  
Libo Jin

Improving the comprehensive utilization of sugars in lignocellulosic biomass is a major challenge for enhancing the economic viability of lignocellulose biorefinement. A robust yeast Pichia kudriavzevii N-X showed excellent performance in ethanol production under high temperature and low pH conditions and was engineered for ᴅ-xylonate production without xylitol generation. The recombinant strain P. kudriavzevii N-X/S1 was employed for sequential production of ᴅ-xylonate and ethanol from ᴅ-xylose, feeding on ᴅ-glucose without pH control in a two-stage strategy of aerobic and shifting micro-aerobic fermentation. Acid-pretreated corncob without detoxification and filtration was used for ᴅ-xylonate production, then simultaneous saccharification and ethanol fermentation was performed with cellulase added at pH 4.0 and at 40 °C. By this strategy, 33.5 g/L ᴅ-xylonate and 20.8 g/L ethanol were produced at yields of 1.10 g/g ᴅ-xylose and 84.3% of theoretical value, respectively. We propose a promising approach for the sequential production of ᴅ-xylonate and ethanol from non-detoxified corncob using a single microorganism.

Sign in / Sign up

Export Citation Format

Share Document