Neutral–Neutral 2-Dimensional Agarose Gel Electrophoresis for Visualization of E. coli DNA Replication Structures

2020 ◽  
pp. 61-72
Author(s):  
Karla A. Mettrick ◽  
Georgia M. Weaver ◽  
Ian Grainge
Keyword(s):  
Dna Replication ◽  
Gel Electrophoresis ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
E Coli

A computer model for the analysis of DNA replication intermediates by two-dimensional agarose gel electrophoresis

Gene ◽  
1998 ◽  
Vol 217 (1-2) ◽  
pp. 41-49 ◽  
Author(s):  
E. Viguera ◽  
A. Rodrı́guez ◽  
P. Hernández ◽  
D.B. Krimer ◽  
O. Trellez ◽  
...  
Keyword(s):  
Dna Replication ◽  
Computer Model ◽  
Gel Electrophoresis ◽  
Agarose Gel ◽  
Two Dimensional ◽  
Agarose Gel Electrophoresis ◽  
Replication Intermediates

scholarly journals Plasmid DNA replication and topology as visualized by two-dimensional agarose gel electrophoresis

Plasmid ◽  
2010 ◽  
Vol 63 (1) ◽  
pp. 1-10 ◽  
Author(s):  
J.B. Schvartzman ◽  
M.L. Martínez-Robles ◽  
P. Hernández ◽  
D.B. Krimer
Keyword(s):  
Dna Replication ◽  
Plasmid Dna ◽  
Gel Electrophoresis ◽  
Agarose Gel ◽  
Two Dimensional ◽  
Agarose Gel Electrophoresis ◽  
And Topology

Characterization and transformation of plasmid pAA-1 found in an antarctic cryptoendolithic bacterium

1996 ◽  
Vol 42 (6) ◽  
pp. 571-576 ◽  
Author(s):  
Christian G. Gliesche ◽  
Marina Jendrach ◽  
Klaus Peissl ◽  
Jörg Siebert ◽  
Peter Hirsch
Keyword(s):  
Plasmid Dna ◽  
Gel Electrophoresis ◽  
Natural Transformation ◽  
Restriction Map ◽  
Agarose Gel ◽  
Dry Valleys ◽  
Agarose Gel Electrophoresis ◽  
Bacterial Isolates ◽  
E Coli ◽  
Natural Genetic Transformation

Sixty-three bacterial isolates from antarctic sandstone samples (Linnaeus Terrace, Asgard Range, McMurdo Dry Valleys) were screened for the presence of plasmid DNA. Twenty-seven percent of all the isolates (mainly Gram-positives) harbored one or more plasmids of low molecular mass (1.1–16.3 kb). Strain AA-341 contained plasmid pAA-1 (2.9 kb), as demonstrated by agarose gel electrophoresis and restriction endonuclease digests. This plasmid conferred resistance to chromium and ampicillin. It was not conjugative, but it could be transferred by electroporation to chromium- and ampicillin-sensitive strains AA-330, AA-338, and E. coli HB101. A restriction map of pAA-1 was constructed with HindIII, SalI, ScaI, AvaII, EcoRI, PvuII, BamHI, and DraI. Electrotransfer of this plasmid from E. coli HB101(pAA-1) to strain AA-330 was demonstrated. By natural genetic transformation, plasmid pAA-1 could be transferred into the sensitive strain AA-334, which thereby became resistant to chromium and ampicillin. The importance of such processes for the colonization of stressed environments is discussed.Key words: Antarctica, cryptoendolithic bacteria, plasmids, resistance to chromium, natural transformation.

Examination of lactococcal bacteriophage c2 DNA replication using two-dimensional agarose gel electrophoresis

Gene ◽  
2001 ◽  
Vol 278 (1-2) ◽  
pp. 101-106 ◽  
Author(s):  
Michael J. Callanan ◽  
Paul W. O'Toole ◽  
Mark W. Lubbers ◽  
Kayla M. Polzin
Keyword(s):  
Dna Replication ◽  
Gel Electrophoresis ◽  
Agarose Gel ◽  
Two Dimensional ◽  
Agarose Gel Electrophoresis

scholarly journals Curing of Escherichia coli Antibiotic Resistance Plasmids by Aspirin

2014 ◽  
Vol 8 (1) ◽  
pp. 30-35
Author(s):  
Ibtesam H. Al Musawi ◽  
Ali A. Al Zaag
Keyword(s):  
Escherichia Coli ◽  
Gel Electrophoresis ◽  
Agarose Gel ◽  
Large Plasmid ◽  
Agarose Gel Electrophoresis ◽  
E Coli ◽  
Resistance Plasmids ◽  
Minimum Number

An isolate of Escherichia coli (E. coli) was isolated from urine sample due to person infected with urinary tract infection (UTI).The isolate was resistant to the following antibiotics: Ampicillin, Cotrimoxazole, Chloramphenicol and Tetracycline. Agarose gel electrophoresis of its plasmids content has revealed the presence of single large plasmid and two small plasmids bands. The large plasmid was conjugative and contained the resistance genes for four antibiotics. In vitro curing of this plasmid was achieved by treatment with salicylic acid (aspirin) with 150,200,250 and 300 µg/ml as indicated by the elimination of resistance, also by absence of large plasmid band following agarose gel electrophoresis. In vivo curing was conducted using New Zealand rabbits. UTI was induced by bacterial inoculation via urethral catheterization. The E. coli from urine samples of the rabbits, the count of which was proportional to the type of treatment. Minimum number of colonies was associated with group treated with metheprim was aspirin 300mg/kg daily dosage. This result may indicate that the side effect of metheprim was in its maximum with aspirin. Survivor bacteria may indicate incomplete exposure to the drug.

scholarly journals Genetic study of Salmonella spp. Producting Betalatimase (ESBLs)

2010 ◽  
Vol 7 (1) ◽  
pp. 254-260
Author(s):  
Baghdad Science Journal
Keyword(s):  
Genetic Study ◽  
Gel Electrophoresis ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Biochemical Tests ◽  
Salmonella Spp ◽  
Small Plasmid ◽  
E Coli ◽  
Extended Spectrum ◽  
Synergy Test

Ten isolates were collected from different clinical sources from laboratory in medicine century . These isolates were belonging to the genus Salmonella depending on morphological and biochemical tests . The antibiotic scussptibility tests against 10 antibiotics were examined , and it was found that the 60% isolates have multiple resistant to antibiotic ,(70%) of isolates were resistant to ampicillin,(50%) were resistant to augmentin ,(40%) were resistant to ceftriaxone ,(20%) were resistant to cefotaxime and (10%) were resistant to ciprofloxacin and tetracycline while all isolates showed sensitivity to piperacillin, imipenem, amikacin and erythromycin .The ability of Salmonela isolates to produce ?-lactamase enzymes were tested using iodometric method , and the results showed that all isolates produced this enzyme.The ability of these isolates to produce Extended-Spectrum ?-lactamase (ESBLs)were also determined by double disc synergy test , only five isolates produced these enzyme. Agarose gel electrophoresis showed that Salmonella isolates ?-lactamase producer have two small plasmid bands . Transformation experiments revealed that these plasmids were capable to transform E. coli MM294, an observation which indicates the ability of these plasmids to show their expression in more than one host.

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