A Prunus necrotic ringspot virus (PNRSV)-Based Viral Vector for Characterization of Gene Functions in Prunus Fruit Trees

2020 ◽  
pp. 155-163
Author(s):  
Hongguang Cui ◽  
Yinzi Li ◽  
Aiming Wang
Keyword(s):  
Viral Vector ◽  
Fruit Trees ◽  
Ringspot Virus ◽  
Gene Functions ◽  
Prunus Necrotic Ringspot Virus

scholarly journals Molecular characterization of Prunus necrotic ringspot virus isolated from rose in Brazil

2015 ◽  
Vol 45 (12) ◽  
pp. 2197-2200 ◽  
Author(s):  
Thor Vinícius Martins Fajardo ◽  
Monique Bezerra Nascimento ◽  
Marcelo Eiras ◽  
Osmar Nickel ◽  
Gilvan Pio-Ribeiro
Keyword(s):  
Amino Acid ◽  
Molecular Characterization ◽  
Molecular Analysis ◽  
Nucleotide Sequences ◽  
Amino Acid Sequences ◽  
Causal Agent ◽  
Ringspot Virus ◽  
Prunus Necrotic Ringspot Virus ◽  
First Time

ABSTRACT: There is no molecular characterization of Brazilian isolates of Prunus necrotic ringspot virus (PNRSV), except for those infecting peach. In this research, the causal agent of rose mosaic was determined and the movement (MP) and coat (CP) protein genes of a PNRSV isolate from rose were molecularly characterized for the first time in Brazil. The nucleotide and deduced amino acid sequences of MP and CP complete genes were aligned and compared with other isolates. Molecular analysis of the MP and CP nucleotide sequences of a Brazilian PNRSV isolate from rose and others from this same host showed highest identities of 96.7% and 98.6%, respectively, and Rose-Br isolate was classified in PV32 group.

Molecular characterization of two prunus necrotic ringspot virus isolates from Canada

2012 ◽  
Vol 157 (5) ◽  
pp. 999-1001 ◽  
Author(s):  
Hongguang Cui ◽  
Ni Hong ◽  
Guoping Wang ◽  
Aiming Wang
Keyword(s):  
Molecular Characterization ◽  
Ringspot Virus ◽  
Prunus Necrotic Ringspot Virus ◽  
Virus Isolates

scholarly journals Development of Real-Time RT-PCR Assay for Detection of Prunus necrotic ringspot virus in Fruit Trees

Plant Disease ◽  
2003 ◽  
Vol 87 (11) ◽  
pp. 1344-1348 ◽  
Author(s):  
S. Marbot ◽  
M. Salmon ◽  
M. Vendrame ◽  
A. Huwaert ◽  
J. Kummert ◽  
...  
Keyword(s):  
Real Time ◽  
Fruit Trees ◽  
Minor Groove ◽  
Diagnostic Techniques ◽  
Ringspot Virus ◽  
Covalent Attachment ◽  
Minor Groove Binder ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Prunus Necrotic Ringspot Virus

A real-time fluorescent reverse-transcriptase polymerase chain reaction (RT-PCR) assay using a short fluorogenic 3′ minor groove binder (MGB) DNA hydrolysis probe was developed for the detection of Prunus necrotic ringspot virus (PNRSV) in stone fruit trees. The covalent attachment of the minor groove binder moiety at the 3′ end of the probe increased the probe target duplex stability and raised the melting temperature to a range suitable for real-time analysis. The real-time RT-PCR assay correlated well with conventional RT-PCR results for the detection of PNRSV. This assay reliably detects PNRSV in bark tissues of dormant cherry and plum trees. Furthermore, it is well adapted for the routine detection of PNRSV because it eliminates one risk of contamination by performing the whole test in a single closed tube. This system may replace the commonly used diagnostic techniques (e.g., woody indicators and immunological tests) to detect this virus.

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