Anther Culture and Chromosome Doubling in Mediterranean Japonica Rice

2021 ◽  
pp. 333-341
Author(s):  
Isidre d’Hooghvorst ◽  
Irene Ferreres ◽  
Salvador Nogués
Keyword(s):  
Anther Culture ◽  
Chromosome Doubling ◽  
Japonica Rice

scholarly journals A Mediterranean japonica rice (Oryza sativa) cultivar improvement through anther culture

Euphytica ◽  
2013 ◽  
Vol 195 (1) ◽  
pp. 31-44 ◽  
Author(s):  
X. Serrat ◽  
M. Cardona ◽  
J. Gil ◽  
A. M. Brito ◽  
L. Moysset ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Anther Culture ◽  
Japonica Rice ◽  
Cultivar Improvement

scholarly journals Colchicine and osmotic stress for improving anther culture efficiency on long grain temperate and tropical japonica rice genotypes

2019 ◽  
Vol 36 (4) ◽  
pp. 269-273
Author(s):  
Irene Ferreres ◽  
Mirari Ortega ◽  
Camilo López-Cristoffanini ◽  
Salvador Nogués ◽  
Xavier Serrat
Keyword(s):  
Osmotic Stress ◽  
Anther Culture ◽  
Japonica Rice ◽  
Tropical Japonica ◽  
Rice Genotypes

scholarly journals Efficient Anther Culture Method of the Japonica Rice Cultivar Koshihikari.

2000 ◽  
Vol 50 (3) ◽  
pp. 197-202 ◽  
Author(s):  
Masaaki Daigen ◽  
Osamu Kawakami ◽  
Yuji Nagasawa
Keyword(s):  
Anther Culture ◽  
Culture Method ◽  
Rice Cultivar ◽  
Japonica Rice

scholarly journals Efficient Anther Culture of F1 Plants Derived from Koshihikari and its Related Cultivars in the Japonica Rice.

2000 ◽  
Vol 50 (3) ◽  
pp. 203-206 ◽  
Author(s):  
Masaaki Daigen ◽  
Yosuke Hoshi ◽  
Noriaki Hashimoto ◽  
Yuji Nagasawa
Keyword(s):  
Anther Culture ◽  
Japonica Rice

scholarly journals Effect of Pepper Types on Obtaining Spontaneous Doubled Haploid Plants via Anther Culture

HortScience ◽  
2015 ◽  
Vol 50 (11) ◽  
pp. 1671-1676 ◽  
Author(s):  
Davut Keleş ◽  
Hasan Pınar ◽  
Atilla Ata ◽  
Hatıra Taşkın ◽  
Serhat Yıldız ◽  
...  
Keyword(s):  
Anther Culture ◽  
Chromosome Doubling ◽  
Haploid Plant ◽  
Naphthaleneacetic Acid ◽  
Green Pepper ◽  
Ploidy Levels ◽  
Doubled Haploidy ◽  
Spontaneous Chromosome ◽  
Future Work ◽  
Haploid Plants

The most successful technique used to obtain haploid plant in pepper is anther culture. The chromosome content of haploid plants can be doubled spontaneously or using colchicine. In this study, we compared the rate of spontaneous doubled haploidy of different pepper types. Seven charleston, six bell, eight capia, and seven green pepper genotypes were used as plant material. Murashige and Skoog (MS) nutrient medium with 4 mg·L−1 naphthaleneacetic acid (NAA), 0.5 mg·L−1 6-benzylaminopurine (BAP), 0.25% activated charcoal, 30 g·L−1 sucrose, and 15 mg·L−1 silver nitrate (AgNO3) was used. Ploidy levels of plants obtained through anther culture were detected using both flow cytometry and simple sequence repeats (SSR) markers. The results showed that different spontaneous doubled haploidy rates were obtained from different pepper types. The highest rate was observed in bell pepper type with 53.4% (mean of six genotypes) of haploid plants undergoing spontaneous chromosome doubling. This was followed by charleston and capia types with 31.9% and 30.4% doubling, respectively. Green pepper type gave the lowest spontaneous doubled haploidy rate with 22.2% doubling. The results obtained from this study will be useful both for future work on haploidy in pepper and for breeding programs.

Chromosome doubling and aneuploidy in anther-derived plants from two winter wheat lines

Genome ◽  
1988 ◽  
Vol 30 (2) ◽  
pp. 177-181 ◽  
Author(s):  
S. G. Metz ◽  
H. C. Sharma ◽  
T. A. Armstrong ◽  
P. N. Mascia
Keyword(s):  
Anther Culture ◽  
Doubled Haploid ◽  
High Frequency ◽  
Chromosome Doubling ◽  
Colchicine Treatment ◽  
Triticum Aestivum L ◽  
Plantlet Regeneration ◽  
Vernalization Requirement ◽  
Diploid Plant ◽  
Wheat Lines

Anther-derived, doubled haploid populations were obtained from two hard red winter wheats, 'Centurk' and NB88. Spontaneous doubling frequency, efficiency of colchicine treatment, and vernalization requirement were evaluated within each population. In cytological evaluation among the regenerates, haploids, diploids, haploid aneuploids, and diploid aneuploids were observed. Most regenerates were either haploid or diploid. The frequency of anthers producing at least one haploid, diploid, or aneuploid was the same for both genotypes. The regenerates from the same anther had the same ploidy level 83 % of the time, suggesting the callus was usually derived from one microspore. Over 60% of the anthers produced at least one diploid plant. The high frequency of spontaneous doubling suggests that chromosome doubling by colchicine treatment could be eliminated. Ninety-eight percent of the colchicine-treated 'Centurk' plants produced seed, while only 43% of the NB88 colchicine-treated plants produced seed. Anther culture did not replace the vernalization requirement. Vernalization was required for uniform flowering. Results indicate that it is feasible to use 'Centurk', NB88, and other genotypes with high callus induction, plantlet regeneration, and colchicine response to efficiently produce doubled haploids.Key words: wheat, Triticum aestivum L., anther culture, doubled haploid, chromosome doubling, aneuploidy, vernalization.

scholarly journals Antimitotic and hormone effects on green double haploid plant production through anther culture of Mediterranean japonica rice

2018 ◽  
Vol 134 (2) ◽  
pp. 205-215 ◽  
Author(s):  
Isidre Hooghvorst ◽  
Eduardo Ramos-Fuentes ◽  
Camilo López-Cristofannini ◽  
Mirari Ortega ◽  
Raimon Vidal ◽  
...  
Keyword(s):  
Anther Culture ◽  
Double Haploid ◽  
Plant Production ◽  
Haploid Plant ◽  
Japonica Rice ◽  
Hormone Effects
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