Transient Transfection and Expression of Eukaryotic Membrane Proteins in Expi293F Cells and Their Screening on a Small Scale: Application for Structural Studies

2021 ◽  
pp. 105-128
Author(s):  
Ganna O. Krasnoselska ◽  
Maud Dumoux ◽  
Nadisha Gamage ◽  
Harish Cheruvara ◽  
James Birch ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Transient Transfection ◽  
Small Scale ◽  
Structural Studies ◽  
Eukaryotic Membrane Proteins

scholarly journals Expression strategies for structural studies of eukaryotic membrane proteins

2016 ◽  
Vol 38 ◽  
pp. 137-144 ◽  
Author(s):  
Joseph A Lyons ◽  
Azadeh Shahsavar ◽  
Peter Aasted Paulsen ◽  
Bjørn Panyella Pedersen ◽  
Poul Nissen
Keyword(s):  
Membrane Proteins ◽  
Structural Studies ◽  
Eukaryotic Membrane Proteins

3D Crystallization and Structural Studies of Integral Membrane Proteins in Lipidic Cubic phases

2000 ◽  
Vol 56 (s1) ◽  
pp. s83-s83
Author(s):  
P. Nollert ◽  
M. L. Chiu ◽  
M. C. Loewen ◽  
A. Royant ◽  
H. Behrhali ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Integral Membrane Proteins ◽  
Structural Studies ◽  
Cubic Phases

scholarly journals Detergent-free extraction, reconstitution and characterization of membrane-anchored cytochrome-b5 in native lipids

2020 ◽  
Vol 56 (48) ◽  
pp. 6511-6514 ◽  
Author(s):  
Bankala Krishnarjuna ◽  
Thirupathi Ravula ◽  
Ayyalusamy Ramamoorthy
Keyword(s):  
High Resolution ◽  
Membrane Proteins ◽  
Cytochrome B5 ◽  
Target Protein ◽  
Structural Studies ◽  
Net Charge

Directly extracted membrane proteins are stable and suitable for functional and high-resolution structural studies; however, the efficacy is modulated by the net charge of the target protein and the polymer used at a given pH.

scholarly journals Structural Studies of Membrane Proteins using Pulsed EPR Spectroscopy

2019 ◽  
Vol 116 (3) ◽  
pp. 56a
Author(s):  
Gary A. Lorigan ◽  
Indra D. Sahu ◽  
Daniel L. Drew ◽  
Gunjan Dixit ◽  
Tanbir Ahammad
Keyword(s):  
Membrane Proteins ◽  
Epr Spectroscopy ◽  
Structural Studies ◽  
Pulsed Epr

scholarly journals NMR Structural Studies of Antimicrobial Peptides as In-Plane Helix of Membrane Proteins

2014 ◽  
Vol 106 (2) ◽  
pp. 192a-193a ◽  
Author(s):  
Yongae Kim ◽  
Ji-Ho Jeong ◽  
Ji-Sun Kim
Keyword(s):  
Membrane Proteins ◽  
Antimicrobial Peptides ◽  
Structural Studies

Regulation of the water channel aquaporin-2 by posttranslational modification

2011 ◽  
Vol 300 (5) ◽  
pp. F1062-F1073 ◽  
Author(s):  
Hanne B. Moeller ◽  
Emma T. B. Olesen ◽  
Robert A. Fenton
Keyword(s):  
Membrane Proteins ◽  
Protein Interactions ◽  
Posttranslational Modifications ◽  
Posttranslational Modification ◽  
Water Channel ◽  
Cellular Functions ◽  
Aquaporin 2 ◽  
Eukaryotic Membrane Proteins ◽  
Insight Into

The cellular functions of many eukaryotic membrane proteins, including the vasopressin-regulated water channel aquaporin-2 (AQP2), are regulated by posttranslational modifications. In this article, we discuss the experimental discoveries that have advanced our understanding of how posttranslational modifications affect AQP2 function, especially as they relate to the role of AQP2 in the kidney. We review the most recent data demonstrating that glycosylation and, in particular, phosphorylation and ubiquitination are mechanisms that regulate AQP2 activity, subcellular sorting and distribution, degradation, and protein interactions. From a clinical perspective, posttranslational modification resulting in protein misrouting or degradation may explain certain forms of nephrogenic diabetes insipidus. In addition to providing major insight into the function and dynamics of renal AQP2 regulation, the analysis of AQP2 posttranslational modification may provide general clues as to the role of posttranslational modification for regulation of other membrane proteins.

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