Heterologous Expression and Affinity Purification of Eukaryotic Membrane Proteins in View of Functional and Structural Studies: The Example of the Sarcoplasmic Reticulum Ca2+-ATPase

2009 ◽  
pp. 247-267 ◽  
Author(s):  
Delphine Cardi ◽  
Cédric Montigny ◽  
Bertrand Arnou ◽  
Marie Jidenko ◽  
Estelle Marchal ◽  
...  
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Membrane Proteins ◽  
Heterologous Expression ◽  
Affinity Purification ◽  
Structural Studies ◽  
Eukaryotic Membrane Proteins

scholarly journals Expression strategies for structural studies of eukaryotic membrane proteins

2016 ◽  
Vol 38 ◽  
pp. 137-144 ◽  
Author(s):  
Joseph A Lyons ◽  
Azadeh Shahsavar ◽  
Peter Aasted Paulsen ◽  
Bjørn Panyella Pedersen ◽  
Poul Nissen
Keyword(s):  
Membrane Proteins ◽  
Structural Studies ◽  
Eukaryotic Membrane Proteins

3D Crystallization and Structural Studies of Integral Membrane Proteins in Lipidic Cubic phases

2000 ◽  
Vol 56 (s1) ◽  
pp. s83-s83
Author(s):  
P. Nollert ◽  
M. L. Chiu ◽  
M. C. Loewen ◽  
A. Royant ◽  
H. Behrhali ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Integral Membrane Proteins ◽  
Structural Studies ◽  
Cubic Phases

scholarly journals Structural membrane proteins and loosely associated proteins of the sarcoplasmic reticulum

1974 ◽  
Vol 139 (3) ◽  
pp. 509-513 ◽  
Author(s):  
A. Margreth ◽  
U. Carraro ◽  
G. Salviati
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Membrane Proteins ◽  
Osmotic Shock ◽  
Protein Composition ◽  
Polyacrylamide Gels ◽  
Associated Proteins

The protein composition of sarcoplasmic-reticulum vesicles, either unpurified or after fractionation on sucrose gradients, and with or without previous osmotic shock and sonication, was investigated by electrophoresis in acid polyacrylamide gels. The pattern of release of loosely bound proteins is discussed with respect to their localization in the interior of the vesicles.

scholarly journals Detergent-free extraction, reconstitution and characterization of membrane-anchored cytochrome-b5 in native lipids

2020 ◽  
Vol 56 (48) ◽  
pp. 6511-6514 ◽  
Author(s):  
Bankala Krishnarjuna ◽  
Thirupathi Ravula ◽  
Ayyalusamy Ramamoorthy
Keyword(s):  
High Resolution ◽  
Membrane Proteins ◽  
Cytochrome B5 ◽  
Target Protein ◽  
Structural Studies ◽  
Net Charge

Directly extracted membrane proteins are stable and suitable for functional and high-resolution structural studies; however, the efficacy is modulated by the net charge of the target protein and the polymer used at a given pH.

scholarly journals Structural Studies of Membrane Proteins using Pulsed EPR Spectroscopy

2019 ◽  
Vol 116 (3) ◽  
pp. 56a
Author(s):  
Gary A. Lorigan ◽  
Indra D. Sahu ◽  
Daniel L. Drew ◽  
Gunjan Dixit ◽  
Tanbir Ahammad
Keyword(s):  
Membrane Proteins ◽  
Epr Spectroscopy ◽  
Structural Studies ◽  
Pulsed Epr

scholarly journals NMR Structural Studies of Antimicrobial Peptides as In-Plane Helix of Membrane Proteins

2014 ◽  
Vol 106 (2) ◽  
pp. 192a-193a ◽  
Author(s):  
Yongae Kim ◽  
Ji-Ho Jeong ◽  
Ji-Sun Kim
Keyword(s):  
Membrane Proteins ◽  
Antimicrobial Peptides ◽  
Structural Studies

scholarly journals An Approach to Heterologous Expression of Membrane Proteins. The Case of Bacteriorhodopsin

PLoS ONE ◽  
2015 ◽  
Vol 10 (6) ◽  
pp. e0128390 ◽  
Author(s):  
Dmitry Bratanov ◽  
Taras Balandin ◽  
Ekaterina Round ◽  
Vitaly Shevchenko ◽  
Ivan Gushchin ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Heterologous Expression
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