NMR Structural Studies of Membrane Proteins

1993 ◽  
pp. 159-189 ◽  
Author(s):  
S. J. Opella ◽  
P. A. McDonnell
Keyword(s):  
Membrane Proteins ◽  
Structural Studies

3D Crystallization and Structural Studies of Integral Membrane Proteins in Lipidic Cubic phases

2000 ◽  
Vol 56 (s1) ◽  
pp. s83-s83
Author(s):  
P. Nollert ◽  
M. L. Chiu ◽  
M. C. Loewen ◽  
A. Royant ◽  
H. Behrhali ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Integral Membrane Proteins ◽  
Structural Studies ◽  
Cubic Phases

scholarly journals Detergent-free extraction, reconstitution and characterization of membrane-anchored cytochrome-b5 in native lipids

2020 ◽  
Vol 56 (48) ◽  
pp. 6511-6514 ◽  
Author(s):  
Bankala Krishnarjuna ◽  
Thirupathi Ravula ◽  
Ayyalusamy Ramamoorthy
Keyword(s):  
High Resolution ◽  
Membrane Proteins ◽  
Cytochrome B5 ◽  
Target Protein ◽  
Structural Studies ◽  
Net Charge

Directly extracted membrane proteins are stable and suitable for functional and high-resolution structural studies; however, the efficacy is modulated by the net charge of the target protein and the polymer used at a given pH.

scholarly journals Structural Studies of Membrane Proteins using Pulsed EPR Spectroscopy

2019 ◽  
Vol 116 (3) ◽  
pp. 56a
Author(s):  
Gary A. Lorigan ◽  
Indra D. Sahu ◽  
Daniel L. Drew ◽  
Gunjan Dixit ◽  
Tanbir Ahammad
Keyword(s):  
Membrane Proteins ◽  
Epr Spectroscopy ◽  
Structural Studies ◽  
Pulsed Epr

scholarly journals NMR Structural Studies of Antimicrobial Peptides as In-Plane Helix of Membrane Proteins

2014 ◽  
Vol 106 (2) ◽  
pp. 192a-193a ◽  
Author(s):  
Yongae Kim ◽  
Ji-Ho Jeong ◽  
Ji-Sun Kim
Keyword(s):  
Membrane Proteins ◽  
Antimicrobial Peptides ◽  
Structural Studies

Aligned Membrane Proteins: Structural Studies

2009 ◽  
Author(s):  
Riqiang Fu ◽  
William W. Brey ◽  
Timothy A. Cross
Keyword(s):  
Membrane Proteins ◽  
Structural Studies

scholarly journals NMR structural studies of membrane proteins

1998 ◽  
Vol 8 (5) ◽  
pp. 640-648 ◽  
Author(s):  
Francesca M Marassi ◽  
Stanley J Opella
Keyword(s):  
Membrane Proteins ◽  
Structural Studies

Stealth carriers for low-resolution structure determination of membrane proteins in solution

2014 ◽  
Vol 70 (2) ◽  
pp. 317-328 ◽  
Author(s):  
Selma Maric ◽  
Nicholas Skar-Gislinge ◽  
Søren Midtgaard ◽  
Mikkel B. Thygesen ◽  
Jürgen Schiller ◽  
...  
Keyword(s):  
Data Analysis ◽  
Membrane Proteins ◽  
Small Angle ◽  
Variation Method ◽  
Structural Studies ◽  
Low Resolution ◽  
Angle Scattering ◽  
Intrinsic Complexity ◽  
Experimental Challenge

Structural studies of membrane proteins remain a great experimental challenge. Functional reconstitution into artificial nanoscale bilayer disc carriers that mimic the native bilayer environment allows the handling of membrane proteins in solution. This enables the use of small-angle scattering techniques for fast and reliable structural analysis. The difficulty with this approach is that the carrier discs contribute to the measured scattering intensity in a highly nontrivial fashion, making subsequent data analysis challenging. Here, an elegant solution to circumvent the intrinsic complexity brought about by the presence of the carrier disc is presented. In combination with small-angle neutron scattering (SANS) and the D2O/H2O-based solvent contrast-variation method, it is demonstrated that it is possible to prepare specifically deuterated carriers that become invisible to neutrons in 100% D2O at the length scales relevant to SANS. These `stealth' carrier discs may be used as a general platform for low-resolution structural studies of membrane proteins using well established data-analysis tools originally developed for soluble proteins.

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