Xylose Reductase Activity of Candida guilliermondii During Xylitol Production by Fed-Batch Fermentation

2002 ◽  
pp. 875-883
Author(s):  
Denise C. G. A. Rodrigues ◽  
Silvio S. Da Silva ◽  
J. B. Almeida E Silva ◽  
Michele Vitolo
Keyword(s):  
Batch Fermentation ◽  
Reductase Activity ◽  
Xylose Reductase ◽  
Candida Guilliermondii ◽  
Xylitol Production ◽  
Fed Batch ◽  
Fed Batch Fermentation

Xylose Reductase Activity of Candida guilliermondiiDuring XylitolProduction by Fed-Batch Fermentation

2002 ◽  
Vol 98-100 (1-9) ◽  
pp. 875-884 ◽  
Author(s):  
Denise C. G. A. Rodrigues ◽  
Silvio S. Da Silva ◽  
J. B. Almeida E. E. Silva ◽  
Michele Vitolo
Keyword(s):  
Batch Fermentation ◽  
Reductase Activity ◽  
Xylose Reductase ◽  
Candida Guilliermondii ◽  
Fed Batch ◽  
Fed Batch Fermentation

scholarly journals Improving xylitol yield by deletion of endogenous xylitol-assimilating genes: a study of industrial Saccharomyces cerevisiae in fermentation of glucose and xylose

2020 ◽  
Vol 20 (8) ◽  
Author(s):  
Bai-Xue Yang ◽  
Cai-Yun Xie ◽  
Zi-Yuan Xia ◽  
Ya-Jing Wu ◽  
Min Gou ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Batch Fermentation ◽  
Xylose Reductase ◽  
Fed Batch ◽  
Fed Batch Fermentation ◽  
Engineered Saccharomyces Cerevisiae ◽  
Sorbitol Dehydrogenases ◽  
Simultaneous Saccharification ◽  
Xylitol Yield ◽  
Endogenous Enzymes

ABSTRACT Engineered Saccharomyces cerevisiae can reduce xylose to xylitol. However, in S.cerevisiae, there are several endogenous enzymes including xylitol dehydrogenase encoded by XYL2, sorbitol dehydrogenases encoded by SOR1/SOR2 and xylulokinase encoded by XKS1 may lead to the assimilation of xylitol. In this study, to increase xylitol accumulation, these genes were separately deleted through CRISPR/Cas9 system. Their effects on xylitol yield of an industrial S. cerevisiae CK17 overexpressing Candida tropicalis XYL1 (encoding xylose reductase) were investigated. Deletion of SOR1/SOR2 or XKS1 increased the xylitol yield in both batch and fed-batch fermentation with different concentrations of glucose and xylose. The analysis of the transcription level of key genes in the mutants during fed-batch fermentation suggests that SOR1/SOR2 are more crucially responsible for xylitol oxidation than XYL2 under the genetic background of S.cerevisiae CK17. The deletion of XKS1 gene could also weaken SOR1/SOR2 expression, thereby increasing the xylitol accumulation. The XKS1-deleted strain CK17ΔXKS1 produced 46.17 g/L of xylitol and reached a xylitol yield of 0.92 g/g during simultaneous saccharification and fermentation (SSF) of pretreated corn stover slurry. Therefore, the deletion of XKS1 gene provides a promising strategy to meet the industrial demands for xylitol production from lignocellulosic biomass.

Influence of pH on the xylose reductase activity of Candida guilliermondii during fed-batch xylitol bioproduction

2002 ◽  
Vol 42 (3) ◽  
pp. 201 ◽  
Author(s):  
Denise Celeste Godoy de Andrade Rodrigues ◽  
Silvio Silvério da Silva ◽  
Michele Vitolo
Keyword(s):  
Reductase Activity ◽  
Xylose Reductase ◽  
Candida Guilliermondii ◽  
Fed Batch ◽  
Influence Of Ph

Optimization of fed-batch fermentation for xylitol production by Candida tropicalis

2002 ◽  
Vol 29 (1) ◽  
pp. 16-19
Author(s):  
J-H Kim ◽  
K-C Han ◽  
Y-H Koh ◽  
Y-W Ryu ◽  
J-H Seo
Keyword(s):  
Candida Tropicalis ◽  
Batch Fermentation ◽  
Xylitol Production ◽  
Fed Batch ◽  
Fed Batch Fermentation

Optimization of fed-batch fermentation for xylitol production by Candida tropicalis

2002 ◽  
Vol 29 (1) ◽  
pp. 16-19 ◽  
Author(s):  
J-H Kim ◽  
K-C Han ◽  
Y-H Koh ◽  
Y-W Ryu ◽  
J-H Seo
Keyword(s):  
Candida Tropicalis ◽  
Batch Fermentation ◽  
Xylitol Production ◽  
Fed Batch ◽  
Fed Batch Fermentation
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