Inhibition of the Intracellular Transformation of Rat Ventral Prostate Androgen Receptor by 3′-Deoxyadenosine

1989 ◽  
pp. 182-198
Author(s):  
Richard A. Hiipakka ◽  
Shutsung Liao
Keyword(s):  
Androgen Receptor ◽  
Ventral Prostate ◽  
Rat Ventral Prostate

MEASUREMENT OF FREE AND OCCUPIED CYTOPLASMIC AND NUCLEAR ANDROGEN RECEPTOR SITES IN RAT VENTRAL PROSTATE GLAND

1977 ◽  
Vol 74 (3) ◽  
pp. 393-404 ◽  
Author(s):  
PETER DAVIES ◽  
PHILIP THOMAS ◽  
KEITH GRIFFITHS
Keyword(s):  
Androgen Receptor ◽  
Specific Binding ◽  
Prostate Gland ◽  
Ventral Prostate ◽  
Reliable Estimate ◽  
Exchange Method ◽  
Receptor Proteins ◽  
Receptor Sites ◽  
Rat Ventral Prostate ◽  
Fold Excess

SUMMARY A method has been developed which allows the estimation of occupied and unoccupied androgen receptor sites in both cytoplasmic and nuclear fractions of rat ventral prostate. The procedure involves precipitation of receptor proteins and incubation of precipitates with labelled 5α-dihydrotestosterone. Uptake of 3H-labelled steroid at 0–4 °C gives an indication of free receptor, whereas binding at a raised temperature (15 °C) allows estimation of occupied receptor. Non-specific binding was measured in the presence of a 100-fold excess of unlabelled 5α-dihydrotestosterone. The exchange method was specific for androgens, and specific binding was detected only in fractions of androgen-dependent tissues. The method can be applied to cytosol, whole nuclei, chromatin and salt-extractable and salt-resistant protein preparations from nuclear fractions, and gives a reliable estimate of total receptor sites when occupied as compared with control measurements of unoccupied sites.

Extraction of androgen–receptor complexes from regions of rat ventral prostate nuclei sensitive or resistant to nucleases

1983 ◽  
Vol 99 (1) ◽  
pp. 51-61 ◽  
Author(s):  
P. Davies
Keyword(s):  
Androgen Receptor ◽  
Binding Sites ◽  
Micrococcal Nuclease ◽  
Ventral Prostate ◽  
Receptor Complex ◽  
Sedimentation Analysis ◽  
Receptor Complexes ◽  
Rat Ventral Prostate ◽  
Profile Characteristic ◽  
Androgen Binding

Rat ventral prostate nuclei contain androgen-binding sites which are susceptible or resistant to excision by endonucleolytic action. Those which were susceptible were associated both with oligonucleosomal and subnucleosomal particles. The sedimentation profile characteristic of a nuclear androgen-receptor complex could be obtained by exhaustive nucleolytic digestion or by treatment of fractions with KCl (0·6 mol/l). Androgen-binding sites resistant to DNAase I were also resistant to KCl, whereas those sites resistant to micrococcal nuclease were partially extractable with KCl. Nuclease-resistant sites could be extracted with heparin (10 mg/ml). Androgen–receptor complexes obtained from nuclease-sensitive or nuclease-resistant regions by extraction with KCl or heparin were indistinguishable by routine sedimentation analysis.

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