Guanyl Nucleotide Regulation of the Liver Glucagon-Sensitive Adenylyl Cyclase System

1977 ◽  
pp. 349-371
Author(s):  
L. Birnbaumer ◽  
T. L. Swartz
Keyword(s):  
Adenylyl Cyclase ◽  
Adenylyl Cyclase System ◽  
Nucleotide Regulation

scholarly journals Khellin, but not 8-methoxypsoralen, inhibits adenylyl cyclase system in HeLa cells

1995 ◽  
Vol 1269 (2) ◽  
pp. 162-166 ◽  
Author(s):  
Anna Di Stefano ◽  
Lorenza Trabalzini ◽  
Rita La Gaetana ◽  
Luca Parente ◽  
Paola Lusini ◽  
...  
Keyword(s):  
Adenylyl Cyclase ◽  
Hela Cells ◽  
Adenylyl Cyclase System

Role of β-Adrenoceptor/Adenylyl Cyclase System in Cardiac Hypertrophy

2012 ◽  
pp. 305-324
Author(s):  
Adriana Adameova ◽  
Paramjit S. Tappia ◽  
Naranjan S. Dhalla
Keyword(s):  
Cardiac Hypertrophy ◽  
Adenylyl Cyclase ◽  
Adenylyl Cyclase System

Immunocytochemical studies of the Gi Protein mediated muscarinic receptor-adenylyl cyclase system

1995 ◽  
pp. 161-168
Author(s):  
Wolfgang Schulze ◽  
Wolf-Peter Wolf ◽  
Michael L. X. Fu ◽  
Rosemarie Morwinski ◽  
Igor B. Buchwalow ◽  
...  
Keyword(s):  
Adenylyl Cyclase ◽  
Muscarinic Receptor ◽  
Adenylyl Cyclase System ◽  
Gi Protein ◽  
Immunocytochemical Studies

Epidermal growth factor upregulates β-adrenergic receptor signaling in a human salivary cell line

2003 ◽  
Vol 284 (5) ◽  
pp. C1164-C1175 ◽  
Author(s):  
Chih-Ko Yeh ◽  
Tazuko K. Hymer ◽  
April L. Sousa ◽  
Bin-Xian Zhang ◽  
Meyer D. Lifschitz ◽  
...  
Keyword(s):  
Growth Factor ◽  
Protein Kinase ◽  
Adenylyl Cyclase ◽  
Adrenergic Receptor ◽  
Mapk Pathway ◽  
Receptor Density ◽  
Adenylyl Cyclase Activity ◽  
Adenylyl Cyclase System ◽  
Epidermal Growth ◽  
Salivary Cell

The effects of epidermal growth factor (EGF) on the β-adrenergic receptor-coupled adenylyl cyclase system were studied in a human salivary cell line (HSY). The β-adrenergic agonist isoproterenol (10−5 M) stimulated adenylyl cyclase activity by ∼2-fold, and the isoproterenol response was increased 1.8-fold after prolonged (48 h) exposure to EGF (5 × 10−10 M). In contrast, enzyme activation via stimulatory prostaglandin receptors and by agents acting on nonreceptor components of the adenylyl cyclase system was not enhanced by EGF. β-Adrenergic receptor density, assessed by binding of the β-adrenergic receptor antagonist (−)-[125I]iodopindolol, was increased threefold after EGF treatment. Competition binding studies with unlabeled antagonists selective for β1- and β2-adrenergic receptor subtypes indicated that the increase in (−)-[125I]iodopindolol binding sites induced by EGF reflected an increased number of β2-adrenergic receptors. Likewise, Northern blot analysis of RNA from EGF-treated cells revealed selective induction of β2-adrenergic receptor mRNA, which was blocked by the RNA synthesis inhibitor actinomycin D. The increase in β-adrenergic receptor density produced by EGF was unaltered after phorbol ester-induced downregulation of protein kinase C (PKC). Enhancement of isoproterenol-responsive adenylyl cyclase activity and phosphorylation of mitogen-activated protein kinase (MAPK) by EGF were both blocked by the MAPK pathway inhibitor PD-98059. The results suggest that in HSY cells EGF enhances β-adrenergic responsiveness by upregulating β2-adrenergic receptor expression at the transcriptional level. Moreover, the stimulatory effect of EGF on β2-adrenergic receptor signaling appears to be mediated by the MAPK pathway and independent of PKC activation.

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