Some Characteristics of the Antigen — Dependent Migration Inhibition Factor

1979 ◽  
pp. 491-496
Author(s):  
J. Pekárek ◽  
J. Krejcí ◽  
L. Rozprimová ◽  
J. Svejcar
Keyword(s):  
Migration Inhibition Factor ◽  
Migration Inhibition ◽  
Inhibition Factor

Production of Leukocyte Migration Inhibition Factor by Lymphocytes of Larynx Cancer Patients Stimulated by Laryngeal Carcinoma Solubilized Membrane Antigens

1982 ◽  
Vol 68 (1) ◽  
pp. 39-46 ◽  
Author(s):  
Giorgio Cortesina ◽  
Giovanni Patrick Cavallo ◽  
Fabio Beatrice ◽  
Alberto Sartoris ◽  
Mario Bussi ◽  
...  
Keyword(s):  
Laryngeal Carcinoma ◽  
Normal Mucosa ◽  
Leukocyte Migration ◽  
Migration Inhibition Factor ◽  
Migration Inhibition ◽  
Inhibition Factor ◽  
Histocompatibility Complex ◽  
Laryngeal Mucosa ◽  
Leukocyte Migration Inhibition ◽  
Membrane Antigens

The production of leukocyte migration inhibition factor (LIF) from lymphocytes after stimulation with 3 M KCl soluble tumor and normal mucosa extracts was investigated in 30 patients with laryngeal carcinoma at different development stages and in 30 normal donors. The experiments were performed in heterologous and autologous systems. In heterologous systems 3 M KCl tumor extracts induced LIF production by heterologous lymphocytes from patients in 91 % of the cases, and normal mucosa extracts induced LIF production by heterologous lymphocytes from patients in 73 % of the cases and from normal donors in 90 % of the cases. In autologous systems 3 M KCl tumor extracts induced LIF production by autologous lymphocytes from the same patients in 65 % of the cases, whereas the normal laryngeal mucosa extracts induced LIF production by the same autologous lymphocytes in the 6 % of the cases. The high positivity percentage of the test in heterologous systems could be related to differences in the major histocompatibility complex. The 65 % test positivity in autologous systems using tumor extracts could be related to the presence of tumor associated antigens.

scholarly journals ALLOANTISERUM-INDUCED INHIBITION OF MIGRATION INHIBITION FACTOR PRODUCTION IN IMMUNE RESPONSE GENE-CONTROLLED IMMUNE SYSTEMS

1974 ◽  
Vol 140 (2) ◽  
pp. 383-395 ◽  
Author(s):  
Shlomo Z. Ben-Sasson ◽  
Ethan Shevach ◽  
Ira Green ◽  
William E. Paul
Keyword(s):  
T Cell ◽  
Guinea Pigs ◽  
Opposite Effect ◽  
Cell Function ◽  
Migration Inhibition Factor ◽  
Immune Response Gene ◽  
Migration Inhibition ◽  
Inhibition Factor ◽  
Controlled Systems

We have previously demonstrated that alloantisera prepared by reciprocal immunization of strain 2 and strain 13 guinea pigs specifically block stimulation of in vitro DNA synthesis in genetically controlled systems. In order to determine whether this blockade extends to other T-lymphocyte functions, we examined the effect of alloantisera on the production of migration inhibition factor (MIF). (2 x 13)F1 guinea pigs were immunized with a DNP derivative of the copolymer of L-glutamic acid and L-lysine (DNP-GL) and with DNP guinea pig albumin (GPA). The response to the former is controlled by a 2-linked Ir gene while that to the latter is mainly controlled by a 13-linked Ir gene. MIF production was assayed by an indirect procedure in which the migrating cell population lacked the histocompatibility antigen against which the alloantiserum was directed. Our results showed that anti-2 serum blocked MIF production by F1 cells in response to DNP-GL but not DNP-GPA while anti-13 serum had the opposite effect. These experiments show that expression of a second major T-cell function is specifically blocked by alloantisera and suggest that Ir-gene products may act as antigen recognition substances on more than one type of T cell.

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