Binding Assays Involving Separation in Aqueous Two-Phase Systems: Partition Affinity Ligand Assay (PALA)

1982 ◽  
pp. 359-367
Author(s):  
B. Mattiasson ◽  
T. G. I. Ling ◽  
M. Ramstorp
Keyword(s):  
Binding Assays ◽  
Two Phase ◽  
Affinity Ligand ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

Surface heterogeneity of bovine sperm revealed by aqueous two-phase partition

1991 ◽  
Vol 11 (5) ◽  
pp. 265-273 ◽  
Author(s):  
Elizabeth J. Cartwright ◽  
Allison Cowin ◽  
Paul T. Sharpe
Keyword(s):  
Surface Properties ◽  
Seminal Fluid ◽  
Surface Heterogeneity ◽  
Two Phase ◽  
Surface Molecules ◽  
Affinity Ligand ◽  
Bovine Sperm ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Two Phase Partition

Ejaculated, bovine sperm have been subjected to multiple partition in aqueous two-phase systems. This partition, carried out in a countercurrent fashion, reveals heterogeneity of the sperm population with respect to surface properties. The sperm, when partitioned in phase systems that detect non-change associated surface properties (change-insensitive) are largely distributed as two distinct populations. In charge-sensitive phase systems (which principally detect cell surface molecules carrying charge) the sperm do not show any obvious surface heterogeneity. Considerable heterogeneity is revealed in affinity-ligand phase systems containing palmitic acid coupled to one of the phase components-poly(ethylene glycol). There is a difference in surface heterogeneity between sperm which have been washed in buffer or left unwashed, direct from the ejaculate. This is indicative of weak adsorption of proteins to the sperm surface in seminal fluid. These results show that bovine ejaculated sperm is a heterogeneous cell population having unequal distributions of a number of different surface molecules.

Continuous purification of antibodies from cell culture supernatant with aqueous two-phase systems: From concept to process

2013 ◽  
Vol 8 (3) ◽  
pp. 352-362 ◽  
Author(s):  
Paula A. J. Rosa ◽  
Ana M. Azevedo ◽  
S. Sommerfeld ◽  
Martina Mutter ◽  
Werner Bäcker ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture Supernatant ◽  
Cell Culture Supernatant ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

Optimized and Scalable Green Extraction of Pristimerin, an Anticancerigen from Mortonia greggii, by Ethanol–Phosphate Aqueous Two-Phase Systems

2021 ◽  
Vol 60 (15) ◽  
pp. 5403-5410
Author(s):  
Martha Alicia González-Félix ◽  
Luis Alberto Mejía-Manzano ◽  
Bertha A. Barba-Dávila ◽  
Sergio O. Serna-Saldívar ◽  
José González-Valdez
Keyword(s):  
Two Phase ◽  
Green Extraction ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

Recovery of value-added products from wastewater using Aqueous Two-Phase Systems – A review

2021 ◽  
Vol 778 ◽  
pp. 146293
Author(s):  
Thivaharan Varadavenkatesan ◽  
Shraddha Pai ◽  
Ramesh Vinayagam ◽  
Arivalagan Pugazhendhi ◽  
Raja Selvaraj
Keyword(s):  
Value Added ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Value Added Products

Fabrication of Microgel Particles with Complex Shape via Selective Polymerization of Aqueous Two-Phase Systems

Small ◽  
2012 ◽  
Vol 8 (15) ◽  
pp. 2356-2360 ◽  
Author(s):  
Shaohua Ma ◽  
Julian Thiele ◽  
Xin Liu ◽  
Yunpeng Bai ◽  
Chris Abell ◽  
...  
Keyword(s):  
Complex Shape ◽  
Two Phase ◽  
Microgel Particles ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

Characterization of Aqueous Two-Phase Systems from Volume and Density Measurements

2016 ◽  
Vol 61 (4) ◽  
pp. 1531-1539 ◽  
Author(s):  
Ehsan Atefi ◽  
Darcy Fyffe ◽  
Kerim B. Kaylan ◽  
Hossein Tavana
Keyword(s):  
Two Phase ◽  
Density Measurements ◽  
Aqueous Two Phase Systems ◽  
Phase Systems
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