Assay of Lipoprotein Lipase in Postheparin Plasma after Suppression of the Hepatic Triglyceride Lipase with Sodium Dodecyl Sulfate

1978 ◽  
pp. 169-182
Author(s):  
Marietta L. Baginsky ◽  
Flora Wu ◽  
W. Virgil Brown
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Lipoprotein Lipase ◽  
Sodium Dodecyl ◽  
Hepatic Triglyceride ◽  
Triglyceride Lipase ◽  
Dodecyl Sulfate ◽  
Hepatic Triglyceride Lipase

Sensitive non-radioisotopic method for measuring lipoprotein lipase and hepatic triglyceride lipase in post-heparin plasma.

1984 ◽  
Vol 30 (5) ◽  
pp. 748-751 ◽  
Author(s):  
S Nozaki ◽  
M Kubo ◽  
Y Matsuzawa ◽  
S Tarui
Keyword(s):  
Lipoprotein Lipase ◽  
Sodium Dodecyl ◽  
Hepatic Triglyceride ◽  
Healthy Men ◽  
Triglyceride Lipase ◽  
Radioisotopic Method ◽  
Heparin Plasma ◽  
The Mean ◽  
Triton X ◽  
Hepatic Triglyceride Lipase

Abstract In this method for measuring lipoprotein lipase (LPL) and hepatic triglyceride lipase (H-TGL) in post-heparin plasma, we determine the released free fatty acids enzymically. After release, they are extracted by Dole 's method (J. Biol. Chem. 235: 2595-2599, 1960), solubilized with Triton X-100, then measured with an enzymic kit (NEFA Kit-K; Nippon Shoji Kaisha Ltd.) after residual turbidity is removed by centrifugation with chloroform. A 5-microL sample of post-heparin plasma suffices to measure the activity of LPL and H-TGL; thus the method is as sensitive as the radioisotopic method. Selective assay of LPL and H-TGL, by adding sodium dodecyl sulfate to inactivate H-TGL or NaCl to inactivate LPL, is also feasible. The mean activities +/- SD of LPL and H-TGL in plasma of normal healthy men were respectively 9.4 +/- 2.3 mumol/h per milliliter (157 +/- 38 U/L) and 20.1 +/- 10.4 (335 +/- 173 U) mumol/h per milliliter (U/L).

Hepatic and lipoprotein lipases selectively assayed in postheparin plasma

1993 ◽  
Vol 39 (2) ◽  
pp. 218-223 ◽  
Author(s):  
A D Henderson ◽  
W Richmond ◽  
R S Elkeles
Keyword(s):  
Sodium Chloride ◽  
Sodium Dodecyl Sulfate ◽  
Lipoprotein Lipase ◽  
Sex Difference ◽  
Hepatic Lipase ◽  
Sodium Dodecyl ◽  
Plasma Samples ◽  
Dodecyl Sulfate ◽  
Normal Adults

Abstract Sensitive, reliable procedures are reported for the selective assay of lipoprotein lipase (LPL) and hepatic lipase (HL) in postheparin plasma samples. LPL is inhibited in the HL assay by inclusion of 0.76 mol/L sodium chloride in the substrate. In the LPL assay, specificity is attained by pretreating the sample with sodium dodecyl sulfate, which selectively denatures HL. This LPL method was validated by direct comparison with a procedure in which HL is inactivated by an antiserum to human HL. We used the described assays to quantify LPL and HL activity in 32 normal adults, demonstrating a clear sex difference for both enzymes. On average, the men displayed higher HL and lower LPL activities than did the women.

scholarly journals Plasma Lipoprotein Lipase and Hepatic Lipase Activities in Friedreich’s Ataxia

1982 ◽  
Vol 9 (2) ◽  
pp. 191-194 ◽  
Author(s):  
D. Blache ◽  
D. Bouthillier ◽  
A. Barbeau ◽  
J. Davignon
Keyword(s):  
Lipoprotein Lipase ◽  
Hepatic Lipase ◽  
Friedreich’S Ataxia ◽  
Friedreich's Ataxia ◽  
Hepatic Triglyceride ◽  
Normal Range ◽  
Triglyceride Lipase ◽  
Plasma Triglycerides ◽  
Control Subjects ◽  
Hepatic Triglyceride Lipase

SUMMARY:Plasma triglycerides although within the normal range have been shown to be higher in Friedreich’s ataxia than in control subjects. To determine whether this difference could be ascribed to a reduced catabolism of triglyceride-rich lipoproteins, the activities of lipoprotein lipase (LPL) and hepatic triglyceride lipase (HL), released into plasma after an heparin injection, were measured in 13 cases of Friedreich’s ataxia and 14 control subjects of comparable signs. LPL was found to be significantly lower in the ataxic patients. Moreover about half of the cases clustered below the normal range for both lipase activities. This subgroup of Friedreich’s patients had significantly higher plasma triglycerides than those with normal lipase activities. Further studies are needed to relate these findings to other characteristics of the disease.

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