On the Mechanism of Endogenous Lipolysis in Rat Heart: A Role of Lysosomes?

1986 ◽  
pp. 39-43 ◽  
Author(s):  
H. Stam ◽  
S. Broekhoven-Schokker ◽  
W. C. Hülsmann
Keyword(s):  
Rat Heart

scholarly journals Superoxide production by NAD(P)H oxidase and mitochondria is increased in genetically obese and hyperglycemic rat heart and aorta before the development of cardiac dysfunction. The role of glucose-6-phosphate dehydrogenase-derived NADPH

2009 ◽  
Vol 297 (1) ◽  
pp. H153-H162 ◽  
Author(s):  
Sabrina Serpillon ◽  
Beverly C. Floyd ◽  
Rakhee S. Gupte ◽  
Shimran George ◽  
Mark Kozicky ◽  
...  
Keyword(s):  
Cardiac Dysfunction ◽  
Rat Heart ◽  
Mitochondrial Respiratory Chain ◽  
Posterior Wall ◽  
Left Ventricular ◽  
Posterior Wall Thickness ◽  
Patients With Diabetes ◽  
Protein Kinase C Inhibitor ◽  
Glucose 6 Phosphate Dehydrogenase

Increased oxidative stress is a known cause of cardiac dysfunction in animals and patients with diabetes, but the sources of reactive oxygen species [e.g., superoxide anion (O2−)] and the mechanisms underlying O2− production in diabetic hearts are not clearly understood. Our aim was to determine whether NADPH oxidase (Nox) is a source of O2− and whether glucose-6-phosphate dehydrogenase (G6PD)-derived NADPH plays a role in augmenting O2− generation in diabetes. We assessed cardiac function, Nox and G6PD activities, NADPH levels, and the activities of antioxidant enzymes in heart homogenates from young (9–11 wk old) Zucker lean and obese (fa/fa) rats. We found that myocardial G6PD activity was significantly higher in fa/fa than in lean rats, whereas superoxide dismutase and glutathione peroxidase activities were decreased ( P < 0.05). O2− levels were elevated (70–90%; P < 0.05) in the diabetic heart, and this elevation was blocked by the Nox inhibitor gp-91ds-tat (50 μM) or by the mitochondrial respiratory chain inhibitors antimycin (10 μM) and rotenone (50 μM). Inhibition of G6PD by 6-aminonicotinamide (5 mM) and dihydroepiandrosterone (100 μM) also reduced ( P < 0.05) O2− production. Notably, the activities of Nox and G6PD in the fa/fa rat heart were inhibited by chelerythrine, a protein kinase C inhibitor. Although we detected no changes in stroke volume, cardiac output, or ejection fraction, left ventricular diameter was slightly increased during diastole and systole, and left ventricular posterior wall thickness was decreased during systole ( P < 0.05) in Zucker fa/fa rats. Our findings suggest that in a model of severe hyperlipidema and hyperglycemia Nox-derived O2− generation in the myocardium is fueled by elevated levels of G6PD-derived NADPH. Similar mechanisms were found to activate O2− production and induce endothelial dysfunction in aorta. Thus G6PD may be a useful therapeutic target for treating the cardiovascular disease associated with type 2 diabetes, if second-generation drugs specifically reducing the activity of G6PD to near normal levels are developed.

Pressure Volume Curves in Arrested Heterotopic Rat Heart Isografts: Role of Improved Myocardial Protection

1999 ◽  
Vol 86 (1) ◽  
pp. 123-129 ◽  
Author(s):  
Joanne P. Starr ◽  
Chao-Xiang Jia ◽  
David G. Rabkin ◽  
Mehrdad M.R. Amirhamzeh ◽  
Joseph P. Hart ◽  
...  
Keyword(s):  
Rat Heart ◽  
Myocardial Protection

scholarly journals ROLE OF SDF-1α/CXCR4 AXIS TO THE CARDIOPROTECTIVE EFFECT OF ISCHEMIC POST-CONDITIONING IN ISOLATED RAT HEART

2013 ◽  
Vol 61 (10) ◽  
pp. E217
Author(s):  
Jeong-Su Kim ◽  
Ju-Hyun Park ◽  
Kook-Jin Chun ◽  
Young-Ho Jang ◽  
June-Hong Kim ◽  
...  
Keyword(s):  
Rat Heart ◽  
Isolated Rat Heart ◽  
Cardioprotective Effect ◽  
Cxcr4 Axis ◽  
Isolated Rat

The role of nitric oxide, reactive oxygen species, and protein kinase C in oxytocin-induced cardioprotection in ischemic rat heart

Peptides ◽  
2012 ◽  
Vol 37 (2) ◽  
pp. 314-319 ◽  
Author(s):  
Mahdieh Faghihi ◽  
Ali Mohammad Alizadeh ◽  
Vahid Khori ◽  
Mostafa Latifpour ◽  
Saeed Khodayari
Keyword(s):  
Nitric Oxide ◽  
Reactive Oxygen Species ◽  
Protein Kinase C ◽  
Protein Kinase ◽  
Rat Heart ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Kinase C

The effects of organophosphate insecticide diazinon on malondialdehyde levels and myocardial cells in rat heart tissue and protective role of vitamin E

2006 ◽  
Vol 86 (2) ◽  
pp. 93-98 ◽  
Author(s):  
Ayse Ogutcu ◽  
Meltem Uzunhisarcikli ◽  
Suna Kalender ◽  
Dilek Durak ◽  
Fatma Bayrakdar ◽  
...  
Keyword(s):  
Vitamin E ◽  
Rat Heart ◽  
Heart Tissue ◽  
Protective Role ◽  
Myocardial Cells ◽  
Organophosphate Insecticide

Histopathological and immunohistochemical alterations in rat heart after thyroidectomy and the role of hemin and ketoconazole in treatment

2012 ◽  
Vol 66 (8) ◽  
pp. 627-632 ◽  
Author(s):  
Ehab Tousson ◽  
Ehab M. Ali ◽  
Wafaa Ibrahim ◽  
Rana M. Ashraf
Keyword(s):  
Rat Heart
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