Free-Flow Electrophoresis Resolves Plasma Membrane Vesicles Isolated by Aqueous Two Phase Partition into Two Populations of Different Sidedness

Author(s):  
Hervé Canut ◽  
Andrew Brightman ◽  
Alain Boudet ◽  
D. James Morré
2016 ◽  
Vol 15 (3) ◽  
pp. 900-913 ◽  
Author(s):  
Roberto de Michele ◽  
Heather E. McFarlane ◽  
Harriet T. Parsons ◽  
Miranda J. Meents ◽  
Jeemeng Lao ◽  
...  

1988 ◽  
Vol 86 (2) ◽  
pp. 631-637 ◽  
Author(s):  
Hervé Canut ◽  
Andrew Brightman ◽  
Alain M. Boudet ◽  
D. James Morré

1994 ◽  
Vol 49 (7-8) ◽  
pp. 447-452 ◽  
Author(s):  
Sabine Lüthje ◽  
José A. Gonzaléz-Reyes ◽  
Placido Navas ◽  
Olaf Döring ◽  
Michael Böttger

Modulation of plasma membrane-bound NADH:hexacyanoferrate III oxidoreductase activities by dicumarol and warfarin was investigated with plasma membrane vesicles of Zea mays L. (cv. Sil Anjou 18) roots, prepared by aqueous two phase partitioning. Vesicles were about 65% right-side out orientated as demonstrated by enzyme latency of vanadate sensitive ATPase activity. Dicumarol or warfarin, respectively, inhibited NADH:hexacyanoferrate III oxidoreductase activity in a concentration-dependent manner and inhibition could be reversed partially by addition of quinones


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