Isolation of plasma membrane fractions from green wheat leaves by free-flow electrophoresis or two-phase partition alone or in combination

1992 ◽  
Vol 84 (1) ◽  
pp. 121-133 ◽  
Author(s):  
Alfred Egger ◽  
D. James Moire ◽  
Gun Sellden ◽  
Anna Stina Sandelius
Keyword(s):  
Plasma Membrane ◽  
Free Flow ◽  
Two Phase ◽  
Phase Partition ◽  
Wheat Leaves ◽  
Two Phase Partition

The application of aqueous two-phase partition to the study of chick limb mesenchymal diversification

Development ◽  
1986 ◽  
Vol 94 (1) ◽  
pp. 267-275
Author(s):  
C. P. Cottrill ◽  
Paul T. Sharpe ◽  
Lewis Wolpert
Keyword(s):  
Pattern Formation ◽  
Limb Development ◽  
Developmental Stages ◽  
Proximal Region ◽  
Limb Bud ◽  
Cell Populations ◽  
Two Phase ◽  
Phase Partition ◽  
Surface Character ◽  
Two Phase Partition

A technique which identifies cells differing in surface character, aqueous two-phase partition using thin-layer countercurrent distribution (TLCCD), has been used to study differentiation and pattern formation in the developing chick limb bud. The TLCCD profiles of cell populations, derived from various regions of morphologically undifferentiated mesenchyme from three different stages of limb development, have been compared. At no stage, or location, has the population been found to be homogeneous. Cells from progress zones and more proximal regions could all be resolved into several populations. The populations from progress zones at three different developmental stages were qualitatively similar but differed in the proportions of cells in each. The most striking differences in cell populations were those obtained from the most proximal region of the limb, closest to the flank, which represents the developmentally most advanced region.

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