Autocrine and Paracrine Effects of the Somatomedins/Insulin-Like Growth Factors

1989 ◽  
pp. 5-23 ◽  
Author(s):  
Judson J. Van Wyk ◽  
P. Kay Lund
Keyword(s):  
Growth Factors ◽  
Paracrine Effects ◽  
Insulin Like Growth Factors

Autocrine and paracrine actions of intestinal fibroblast-derived insulin-like growth factors

1999 ◽  
Vol 276 (4) ◽  
pp. G817-G827 ◽  
Author(s):  
James G. Simmons ◽  
Jolanta B. Pucilowska ◽  
P. Kay Lund
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Epithelial Cells ◽  
Mitogenic Activity ◽  
Ribonuclease Protection Assay ◽  
Rt Pcr ◽  
Paracrine Effects ◽  
Igf I ◽  
Ribonuclease Protection ◽  
Insulin Like Growth Factors

Paracrine and autocrine actions of the insulin-like growth factors (IGFs) are inferred by local expression within the bowel. CCD-18Co cells, IEC-6 cells, and immunoneutralization were used to analyze whether IGFs have direct autocrine or paracrine effects on proliferation of cultured intestinal fibroblasts and epithelial cells. Growth factor expression was analyzed by ribonuclease protection assay and RT-PCR. Extracellular matrix (ECM) was analyzed for effects on cell proliferation. CCD-18Co cells express IGF-II mRNAs and low levels of IGF-I mRNA. Conditioned medium from CCD-18Co cells (CCD-CM) stimulated proliferation of IEC-6 and CCD-18Co cells. Neutralization of IGF immunoreactivity in CCD-CM reduced but did not abolish this effect. RT-PCR and immunoneutralization demonstrated that other growth factors contribute to mitogenic activity of CCD-CM. Preincubation of CCD-CM with ECM prepared from IEC-6 or CCD-18Co cells reduced its mitogenic activity. ECM from CCD-18Co cells enhanced growth factor-dependent proliferation of IEC-6 cells. IEC-6 cell ECM inhibited IGF-I action on CCD-18Co cells. We conclude that IGF-II is a potent autocrine mitogen for intestinal fibroblasts. IGF-II interacts with other fibroblast-derived growth factors and ECM to stimulate proliferation of intestinal epithelial cells in a paracrine manner.

Insulin-like growth factors in vitreous. Studies in control and diabetic subjects with neovascularization

Diabetes ◽  
1986 ◽  
Vol 35 (4) ◽  
pp. 416-420 ◽  
Author(s):  
M. Grant ◽  
B. Russell ◽  
C. Fitzgerald ◽  
T. J. Merimee
Keyword(s):  
Growth Factors ◽  
Insulin Like Growth Factors

Von Willebrand factor (VIII R:Ag), fibronectin, and insulin-like growth factors I and II in diabetic retinopathy and nephropathy

Diabetes ◽  
1984 ◽  
Vol 33 (2) ◽  
pp. 125-129 ◽  
Author(s):  
R. P. Lamberton ◽  
A. D. Goodman ◽  
A. Kassoff ◽  
C. L. Rubin ◽  
D. H. Treble ◽  
...  
Keyword(s):  
Diabetic Retinopathy ◽  
Growth Factors ◽  
Factor Viii ◽  
Von Willebrand Factor ◽  
Von Willebrand ◽  
Willebrand Factor ◽  
Insulin Like Growth Factors

scholarly journals The insulin-like growth factors: ligands, binding proteins and receptors

2021 ◽  
pp. 101245
Author(s):  
Derek LeRoith ◽  
Jeff MP. Holly ◽  
Briony Forbes
Keyword(s):  
Growth Factors ◽  
Binding Proteins ◽  
Insulin Like Growth Factors
Sign in / Sign up

Export Citation Format

Share Document