Effects of Volatile Anesthetics on Cardiac Sarcoplasmic Reticulum as Determined in Intact Cells

1991 ◽  
pp. 143-154
Author(s):  
David M. Wheeler ◽  
Ana Katz ◽  
R. Todd Rice
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Volatile Anesthetics ◽  
Intact Cells ◽  
Cardiac Sarcoplasmic Reticulum

Cs+ inhibits spontaneous Ca2+ release from sarcoplasmic reticulum of skinned cardiac myocytes

1998 ◽  
Vol 275 (2) ◽  
pp. H422-H430 ◽  
Author(s):  
Makoto Kawai ◽  
Munir Hussain ◽  
Clive H. Orchard
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Cardiac Myocytes ◽  
Ventricular Myocytes ◽  
Cyclopiazonic Acid ◽  
Bathing Solution ◽  
Spontaneous Release ◽  
Inhibitory Effects ◽  
Intact Cells ◽  
Cardiac Sarcoplasmic Reticulum ◽  
Fura 2

The effect of Cs+ on the function of the cardiac sarcoplasmic reticulum (SR) has been investigated in skinned cardiac myocytes. Isolated rat ventricular myocytes were permeabilized using saponin and then perfused with a solution containing 150 nmol/l Ca2+ and 10 μmol/l fura 2. Fura 2 fluorescence from the skinned cell was monitored to assess SR Ca2+ release. The frequency of spontaneous Ca2+ release from the SR decreased when K+ in the bathing solution was completely replaced with Cs+. Cs+ had little effect on the amplitude of spontaneous release but prolonged both the rise time and decay time. The SR Ca2+ content, assessed by application of caffeine, was reduced in the Cs+ solution. Cyclopiazonic acid produced effects similar to those of Cs+. Extracellular Cs+ (20 mmol/l) increased the amplitude of the Ca2+ transient and the SR Ca2+ content in intact field-stimulated cells but had little effect on the Ca2+ transient when the amplitude and duration of depolarization were kept constant using voltage clamp. These data suggest that Cs+ slows Ca2+ movement across the SR membrane, possibly by blocking the SR K+ channel, but has additional effects in intact cells that overcome its inhibitory effects on the SR.

A658 HEART RATE DEPENDENT EFFECTS OF VOLATILE ANESTHETICS ON THE CARDIAC SARCOPLASMIC RETICULUM

1990 ◽  
Vol 73 (3A) ◽  
pp. NA-NA
Author(s):  
Th. Winguth ◽  
N. Roewer ◽  
D. Hünerbein ◽  
E. Rumberger ◽  
J. Schulte am Esch
Keyword(s):  
Heart Rate ◽  
Sarcoplasmic Reticulum ◽  
Volatile Anesthetics ◽  
Cardiac Sarcoplasmic Reticulum ◽  
Rate Dependent

THE EFFECT OF VOLATILE ANESTHETICS ON THE PH DEPENDENCE OF CALCIUM UPTAKE BY CARDIAC SARCOPLASMIC RETICULUM

1986 ◽  
Vol 65 (Supplement 3A) ◽  
pp. A412
Author(s):  
E. S. Casella ◽  
N. D.A. Suite ◽  
Y. I. Fisher ◽  
T. J. J. Blanck
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Calcium Uptake ◽  
Ph Dependence ◽  
Volatile Anesthetics ◽  
Cardiac Sarcoplasmic Reticulum

The Effect of Volatile Anesthetics on the pH Dependence of Calcium Uptake by Cardiac Sarcoplasmic Reticulum

1987 ◽  
Vol 67 (3) ◽  
pp. 386-390 ◽  
Author(s):  
Eugenie Shalhoub Casella ◽  
Nicholas D. A. Suite ◽  
Yvonne I. Fisher ◽  
Thomas J. J. Blanck
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Calcium Uptake ◽  
Ph Dependence ◽  
Volatile Anesthetics ◽  
Cardiac Sarcoplasmic Reticulum

A Reproducible Selective Stain for Cardiac Sarcoplasmic Reticulum

1974 ◽  
Vol 32 ◽  
pp. 214-215
Author(s):  
R. A. Waugh ◽  
J. R. Sommer
Keyword(s):  
Complex System ◽  
Electron Microscope ◽  
Sarcoplasmic Reticulum ◽  
Transmission Electron Microscope ◽  
Electron Microscopic ◽  
Cardiac Sarcoplasmic Reticulum ◽  
Transmission Electron

Cardiac sarcoplasmic reticulum (SR) is a complex system of intracellular tubules that, due to their small size and juxtaposition to such electron-dense structures as mitochondria and myofibrils, are often inconspicuous in conventionally prepared electron microscopic material. This study reports a method with which the SR is selectively “stained” which facilitates visualizationwith the transmission electron microscope.

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