Use of Isolated Membrane Vesicles in Transport Studies

Transport ◽  
1975 ◽  
pp. 117-162 ◽  
Author(s):  
Joy Hochstadt ◽  
Dennis C. Quinlan ◽  
Richard L. Rader ◽  
Chien-Chung Li ◽  
Diana Dowd
Keyword(s):  
Membrane Vesicles ◽  
Transport Studies ◽  
Isolated Membrane Vesicles

Rotavirus interaction with isolated membrane vesicles.

1994 ◽  
Vol 68 (6) ◽  
pp. 4009-4016 ◽  
Author(s):  
M C Ruiz ◽  
S R Alonso-Torre ◽  
A Charpilienne ◽  
M Vasseur ◽  
F Michelangeli ◽  
...  
Keyword(s):  
Membrane Vesicles ◽  
Isolated Membrane Vesicles

scholarly journals Substrate specificity of the ileal and the hepatic Na+/bile acid cotransporters of the rabbit. I. Transport studies with membrane vesicles and cell lines expressing the cloned transporters

1999 ◽  
Vol 40 (9) ◽  
pp. 1604-1617 ◽  
Author(s):  
Werner Kramer ◽  
Siegfried Stengelin ◽  
Karl-Heinz Baringhaus ◽  
Alfons Enhsen ◽  
Hubert Heuer ◽  
...  
Keyword(s):  
Bile Acid ◽  
Substrate Specificity ◽  
Cell Lines ◽  
Membrane Vesicles ◽  
Transport Studies

Transport Studies in Bacterial Membrane Vesicles

Science ◽  
1974 ◽  
Vol 186 (4167) ◽  
pp. 882-892 ◽  
Author(s):  
H. R. Kaback
Keyword(s):  
Membrane Vesicles ◽  
Bacterial Membrane ◽  
Transport Studies

Transport of L-proline by luminal membrane vesicles from pars recta of rabbit proximal tubule

1988 ◽  
Vol 254 (5) ◽  
pp. F628-F633
Author(s):  
H. Roigaard-Petersen ◽  
C. Jacobsen ◽  
M. I. Sheikh
Keyword(s):  
Active Site ◽  
Membrane Vesicles ◽  
Rabbit Kidney ◽  
Renal Transport ◽  
High Affinity ◽  
Luminal Membrane ◽  
Transport Studies ◽  
Straight Tubules ◽  
Pars Recta ◽  
Maximal Capacity

The mechanism of renal transport of L-proline by luminal membrane vesicles prepared from proximal straight tubules (pars recta) of rabbit kidney was investigated. The following picture emerges from transport studies: an electrogenic and Na+-requiring system confined to this region of nephron exists for transport of L-proline with a high affinity (Km = 0.16 mM) and low capacity (Vmax = 3.5 nmol.mg protein-1.15 S-1). Lowering the pH from 7.5 to 5.5 increased the affinity (Km lowered from 0.16 mM at pH 7.5 to 0.08 mM at pH 5.5) without changing the maximal capacity of this system. Modification of histidyl residues of the intact luminal membrane vesicles by diethyl-pyrocarbonate (DEP) completely abolished the transient renal accumulation of L-proline. Simultaneous presence of Na+ and L-proline (10 mM) protects against DEP inactivation of renal transport of radioactive L-proline. We propose that a histidyl residue may be at or close to the active site of L-proline transporter in vesicles from the pars recta.

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