Enhanced therapeutic efficacy of a novel self-micellizing nanoformulation-loading fisetin against acetaminophen-induced liver injury

Aim: To evaluate the feasibility of using dipotassium glycyrrhizinate (DG) as a nanocarrier-loading fisetin (FIT) with strengthened treatment efficacies against liver injury induced by acetaminophen overdose. Methods: DG–FIT was prepared, and its efficacy against liver injury induced by acetaminophen overdose was evaluated. Results: DG–FIT was successfully fabricated with excellent physicochemical properties. DG–FIT could be easily dissolved in water to form a clear micelle solution with high FIT encapsulation efficiency. FIT in DG–FIT exhibited a dramatically improved aqueous solubility. DG–FIT improved intestinal permeation. Regarding in vivo efficacies, DG–FIT exhibited significant effect against acetaminophen overdose by suppressing oxidative stress and proinflammatory cytokines involved. Conclusion: DG–FIT formulation possibly represents a promising method for strengthening the efficacy of FIT against acetaminophen-induced liver injury.

REVIEWING THE THERAPEUTIC ACTIVITY OF VITAMIN D WITH ANTIBIOTIC

This article is an examination of the Rar The scientific development and subsequent “Vitamin D and antibodies” continues to influence the researchers all over the globe today. This article examines the research done and published by researchers and scientists. Consideration of current trends and data in scientific queries and demonstrates further aspects of Therapeutic activity of vitamin D with antibiotic. Additionally, this article explores options for vitamin D with cefuroxime. Cefuroxime has been shown to severely impede fracture healing in rats, thus it should be avoided during fracture healing. Vitamin D with Amoxicillin. As it enhancers absorption of many intestinal permeation enhancers, it is very approachable for the pharma sector to invest and develop certain drugs without the fear of toxicity or insufficiency. Vitamin D with azithromycin. vitamin D selectively inhibits inflammatory cytokines, reducing the infiltration of leukocytes, neutrophils, monocytes / macrophages, and mast cells at the site of inflammation. Historical large-scale use and excellent safety record of azithromycin in respiratory infections are reliable. Hydroxychloroquine is a proposed new therapeutic agent in COVID 19 (viral pneumonia). Key words: lyme diseases, intestinal permeation, COVID, inflammatory cytokines, warfarin therapy

Oleacein Intestinal Permeation and Metabolism in Rats Using an In Situ Perfusion Technique

Oleacein (OLEA) is one of the most important phenolic compounds in extra virgin olive oil in terms of concentration and health-promoting properties, yet there are insufficient data on its absorption and metabolism. Several non-human models have been developed to assess the intestinal permeability of drugs, among them, single-pass intestinal perfusion (SPIP), which is commonly used to investigate the trans-membrane transport of drugs in situ. In this study, the SPIP model and simultaneous luminal blood sampling were used to study the absorption and metabolism of OLEA in rats. Samples of intestinal fluid and mesenteric blood were taken at different times and the ileum segment was excised at the end of the experiment for analysis by LC–ESI–LTQ–Orbitrap–MS. OLEA was mostly metabolized by phase I reactions, undergoing hydrolysis and oxidation, and metabolite levels were much higher in the plasma than in the lumen. The large number of metabolites identified and their relatively high abundance indicates an important intestinal first-pass effect during absorption. According to the results, OLEA is well absorbed in the intestine, with an intestinal permeability similar to that of the highly permeable model compound naproxen. No significant differences were found in the percentage of absorbed OLEA and naproxen (48.98 ± 12.27% and 43.96 ± 7.58%, respectively).