Data Collection from Very Thin HLA Crystals Using Synchrotron Radiation

Some of the data collection details for compound (VIII) were incorrectly given in Table 1 of Godino Salido et al. (2004). The data for compound VIII in this paper were collected using synchrotron radiation at the Daresbury SRS station 9.8, λ = 0.6935 Å (Cernik et al., 1997; Clegg, 2000). The data were collected using a Bruker SMART 1K CCD diffractometer using ω rotation with narrow frames. The computer program used in the data collection was SMART (Bruker, 2001) and for cell refinement and data reduction SAINT (Bruker, 2001).

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Protein Crystallography with Synchrotron Radiation I. General Discussion and High Resolution Data Collection

Synchrotron Radiation Applied to Biophysical and Biochemical Research ◽

10.1007/978-1-4684-3590-0_26 ◽

1979 ◽

pp. 349-359

Author(s):

R. Fourme

Keyword(s):

High Resolution ◽

Synchrotron Radiation ◽

Data Collection ◽

Protein Crystallography ◽

High Resolution Data ◽

Resolution Data

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Protein microcrystallography using synchrotron radiation

IUCrJ ◽

10.1107/s2052252517008193 ◽

2017 ◽

Vol 4 (5) ◽

pp. 529-539 ◽

Cited By ~ 30

Author(s):

Masaki Yamamoto ◽

Kunio Hirata ◽

Keitaro Yamashita ◽

Kazuya Hasegawa ◽

Go Ueno ◽

...

Keyword(s):

Synchrotron Radiation ◽

Data Collection ◽

Diffraction Data ◽

Structure Determination ◽

Structural Features ◽

X Ray ◽

Data Collection Strategy ◽

Technological Developments ◽

Novel Method

The progress in X-ray microbeam applications using synchrotron radiation is beneficial to structure determination from macromolecular microcrystals such as smallin mesocrystals. However, the high intensity of microbeams causes severe radiation damage, which worsens both the statistical quality of diffraction data and their resolution, and in the worst cases results in the failure of structure determination. Even in the event of successful structure determination, site-specific damage can lead to the misinterpretation of structural features. In order to overcome this issue, technological developments in sample handling and delivery, data-collection strategy and data processing have been made. For a few crystals with dimensions of the order of 10 µm, an elegant two-step scanning strategy works well. For smaller samples, the development of a novel method to analyze multiple isomorphous microcrystals was motivated by the success of serial femtosecond crystallography with X-ray free-electron lasers. This method overcame the radiation-dose limit in diffraction data collection by using a sufficient number of crystals. Here, important technologies and the future prospects for microcrystallography are discussed.

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Atomic Resolution Data Collection at Synchrotron Radiation Facilities

Seibutsu Butsuri ◽

10.2142/biophys.40.s3_4 ◽

2000 ◽

Vol 40 (supplement) ◽

pp. S3

Author(s):

S. Adachi

Keyword(s):

Synchrotron Radiation ◽

Data Collection ◽

Atomic Resolution ◽

Resolution Data

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A method to stabilize the incident X-ray energy for anomalous diffraction measurements

Journal of Synchrotron Radiation ◽

10.1107/s1600577517006312 ◽

2017 ◽

Vol 24 (4) ◽

pp. 781-786

Author(s):

Wenjia Wang ◽

Xiaoyun Yang ◽

Guangcai Chang ◽

Pengfei An ◽

Kewen Cha ◽

...

Keyword(s):

Synchrotron Radiation ◽

Data Collection ◽

Absorption Edge ◽

Feedback Loop ◽

Inflection Point ◽

Single Crystal Diffraction ◽

Double Crystal ◽

Anomalous Diffraction ◽

X Ray ◽

Double Crystal Monochromator

A method to calibrate and stabilize the incident X-ray energy for anomalous diffraction data collection is provided and has been successfully used at the single-crystal diffraction beamline 1W2B at the Beijing Synchrotron Radiation Facilities. Employing a feedback loop to control the movement of the double-crystal monochromator, this new method enables the incident X-ray energy to be kept within a 0.2 eV range at the inflection point of the absorption edge.

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