In Vitro Synthesis, Transport, and Assembly of Ribulose 1, 5-Bisphosphate Carboxylase Subunits

1978 ◽  
pp. 325-347 ◽  
Author(s):  
Nam-Hai Chua ◽  
Gregory W. Schmidt
Keyword(s):  
Bisphosphate Carboxylase ◽  
In Vitro Synthesis ◽  
Ribulose 1

scholarly journals DEVELOPMENT OF CARBOXYLATION CAPACITY IN TISSUE-CULTURED STRAWBERRY FOLLOWING TRANSPLANTING.

HortScience ◽  
1991 ◽  
Vol 26 (5) ◽  
pp. 481f-481
Author(s):  
Yves Desjardins ◽  
André Gosselin
Keyword(s):  
De Novo ◽  
Carbon Assimilation ◽  
Carbon Dioxide Concentration ◽  
Total Activity ◽  
Bisphosphate Carboxylase ◽  
Ribulose 1 ◽  
Different Origins ◽  
Photosynthetic Carbon Assimilation ◽  
Negligible Contribution

Strawberry plantlets (Fragaria X ananassa Duch. cv. Kent) were submitted to a factorial arrangement of 2 photosynthetic photon fluxes (PPF) (80 and 150 μmol·m-2·s-1, PAR) and 2 CO2 concentrations (330 and 3000 ppm) during the in vitro rooting stage. Leaves were tagged and placed in a growth chamber tor acclimatization. Photosynthetic capability of leaves from different origins was determined by measuring the initial and total activity of Ribulose-1, 5-bisphosphate carboxylase/oxygenase (rubisco), but the contribution of Phosphoenolpyruvate carboxylase (PEPCase) to fixation was also examined High CO2 concentration and PPF significantly increased fresh weight and surface area in vitro and after 4 weeks ex vitro. Improved growth was not the result of increased autotrophy in vitro since initial rubisco activity was 10 times lower than that of de novo formed leaves and declined under high CO2 and PPF. Carbon dioxide concentration and PPF had no effect on total activity of rubisco. Low activation state and total activity of rubisco in in vitro leaves is the cause of poor photosynthetic activity in vitro Persistent in vitro leaves after 4 weeks of acclimatization did not have higher total activity of rubisco, but the activation state was 4 times larger than the corresponding activity in vitro which might thus provide for non-negligible contribution to photosynthetic carbon assimilation. The possible inhibition of photosynthesis by the presence of sugar in the medium is discussed.

Three-dimensional distribution of ribulose-1, 5-bisphosphate carboxylase/oxygemase during the early development of proplastids in dark-grown Euglena cells transferred to an inorganic medium

1990 ◽  
Vol 48 (3) ◽  
pp. 906-907
Author(s):  
Tomoko Ehara ◽  
Shuji Sumida ◽  
Tetsuaki Osafune ◽  
Eiji Hase
Keyword(s):  
Cell Suspension ◽  
Euglena Gracilis ◽  
Carbon Materials ◽  
Three Dimensional ◽  
Peripheral Region ◽  
Plastid Development ◽  
Bisphosphate Carboxylase ◽  
Inorganic Medium ◽  
Ribulose 1 ◽  
Dimensional Distribution

As shown previously, Euglena cells grown in Hutner’s medium in the dark without agitation accumulate wax as well as paramylum, and contain proplastids showing no internal structure except for a single prothylakoid existing close to the envelope. When the cells are transferred to an inorganic medium containing ammonium salt and the cell suspension is aerated in the dark, the wax was oxidatively metabolized, providing carbon materials and energy 23 for some dark processes of plastid development. Under these conditions, pyrenoid-like structures (called “pro-pyrenoids”) are formed at the sites adjacent to the prolamel larbodies (PLB) localized in the peripheral region of the proplastid. The single prothylakoid becomes paired with a newly formed prothylakoid, and a part of the paired prothylakoids is extended, with foldings, in to the “propyrenoid”. In this study, we observed a concentration of RuBisCO in the “propyrenoid” of Euglena gracilis strain Z using immunoelectron microscopy.

In vitro synthesis of enzymatically active HIV-1 protease for rapid phenotypic resistance profiling

2005 ◽  
Vol 32 (4) ◽  
pp. 294-299 ◽  
Author(s):  
Dieter Hoffmann ◽  
Bernd Buchberger ◽  
Cordula Nemetz
Keyword(s):  
In Vitro Synthesis ◽  
Phenotypic Resistance ◽  
Hiv 1
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