Superoxide Radical and Superoxide Dismutases: Threat and Defense

1980 ◽  
pp. 429-446 ◽  
Author(s):  
Karen Brawn ◽  
Irwin Fridovich
Keyword(s):  
Superoxide Radical ◽  
Superoxide Dismutases

scholarly journals Reactions of superoxide dismutases with HS − /H 2 S and superoxide radical anion: An in vitro EPR study

Nitric Oxide ◽  
2015 ◽  
Vol 51 ◽  
pp. 19-23 ◽  
Author(s):  
Bojana Bolić ◽  
Ana Mijušković ◽  
Ana Popović-Bijelić ◽  
Aleksandra Nikolić-Kokić ◽  
Snežana Spasić ◽  
...  
Keyword(s):  
Radical Anion ◽  
Superoxide Radical ◽  
Superoxide Dismutases ◽  
Superoxide Radical Anion

Superoxide radical generation and Mn- and Cu-Zn superoxide dismutases activities in human leukemic cells

2003 ◽  
Vol 21 (1) ◽  
pp. 11-16 ◽  
Author(s):  
Masahiko Kato ◽  
Hisanori Minakami ◽  
Minoru Kuroiwa ◽  
Yasuyuki Kobayashi ◽  
Shigeru Oshima ◽  
...  
Keyword(s):  
Superoxide Radical ◽  
Leukemic Cells ◽  
Superoxide Dismutases ◽  
Radical Generation ◽  
Human Leukemic Cells

scholarly journals Cytosolic Fe-superoxide dismutase safeguardsTrypanosoma cruzifrom macrophage-derived superoxide radical

2019 ◽  
Vol 116 (18) ◽  
pp. 8879-8888 ◽  
Author(s):  
Alejandra Martínez ◽  
Carolina Prolo ◽  
Damián Estrada ◽  
Natalia Rios ◽  
María Noel Alvarez ◽  
...  
Keyword(s):  
Superoxide Radical ◽  
Parasite Burden ◽  
Anion Channel ◽  
Protonated Form ◽  
Superoxide Dismutases ◽  
Wild Type ◽  
Ph Values ◽  
Perhydroxyl Radical ◽  
Intracellular Proliferation

Trypanosoma cruzi, the causative agent of Chagas disease (CD), contains exclusively Fe-dependent superoxide dismutases (Fe-SODs). DuringT. cruziinvasion to macrophages, superoxide radical (O2•−) is produced at the phagosomal compartment toward the internalized parasite via NOX-2 (gp91-phox) activation. In this work,T. cruzicytosolic Fe-SODB overexpressers (pRIBOTEX–Fe-SODB) exhibited higher resistance to macrophage-dependent killing and enhanced intracellular proliferation compared with wild-type (WT) parasites. The higher infectivity of Fe-SODB overexpressers compared with WT parasites was lost in gp91-phox−/−macrophages, underscoring the role of O2•−in parasite killing. Herein, we studied the entrance of O2•−and its protonated form, perhydroxyl radical [(HO2•); pKa= 4.8], toT. cruziat the phagosome compartment. At the acidic pH values of the phagosome lumen (pH 5.3 ± 0.1), high steady-state concentrations of O2•−and HO2•were estimated (∼28 and 8 µM, respectively). Phagosomal acidification was crucial for O2•−permeation, because inhibition of the macrophage H+-ATPase proton pump significantly decreased O2•−detection in the internalized parasite. Importantly, O2•−detection, aconitase inactivation, and peroxynitrite generation were lower in Fe-SODB than in WT parasites exposed to external fluxes of O2•−or during macrophage infections. Other mechanisms of O2•−entrance participate at neutral pH values, because the anion channel inhibitor 5-nitro-2-(3-phenylpropylamino) benzoic acid decreased O2•−detection. Finally, parasitemia and tissue parasite burden in mice were higher in Fe-SODB–overexpressing parasites, supporting the role of the cytosolic O2•−-catabolizing enzyme as a virulence factor for CD.

Four cytosolic-type CuZn-superoxide dismutases in germinating seeds of Pinus sylvestris

1994 ◽  
Vol 92 (3) ◽  
pp. 443-450 ◽  
Author(s):  
Steffen Streller ◽  
Stanislaw Karpinski ◽  
Jan-Erik Hallgren ◽  
Gunnar Wingsle
Keyword(s):  
Pinus Sylvestris ◽  
Superoxide Dismutases ◽  
Germinating Seeds

Tissue chemiluminescence as a method of evaluation of superoxide radical producing ability of mitochondria

2016 ◽  
pp. 49-55 ◽  
Author(s):  
A.A. Dzhatdoeva ◽  
◽  
A.M. Polimova ◽  
E.V. Proskurnina ◽  
Yu.A. Vladimirov ◽  
...  
Keyword(s):  
Superoxide Radical ◽  
Method Of Evaluation

Antioxidant capacity of hydroalcoholic extracts of Grewia serrulata DC and Grewia nervosa (Lour.) Panigrahi

2018 ◽  
Vol 9 (1) ◽  
pp. 121
Author(s):  
Mentham Ramesh ◽  
Chandu Babu Rao
Keyword(s):  
Total Phenolic Content ◽  
Superoxide Radical ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Flavonoid ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content

The current investigation is intended to evaluate the content of phytochemical constituents and antioxidant potential of hydroalcoholic extracts of stem and root of Grewia serrulata DC (HAESGS & HAERGS) and leaf and bark of Grewia Nervosa (Lour.) panigrahi (HAELGN & HAEBGN). Initially, all the extracts at different concentrations were estimated for their total phenolic content and total flavonoid content. The study was further extended for their antioxidant potential evaluation using various in vitro methods such as 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical and superoxide radical scavenging assays. The total phenolic content (mg gallic acid equivalent per gram of extract) was high in HAELGN (170.82±0.19) and HAERGS (123.00±0.48) than HAESGS (111.2±0.26) and HAEBGN (119.60±0.23). The total flavonoid content (mg quercetin equivalent per gram) is greater in HAERGS (71.24±0.50) and HAESGS (65.68±0.27) than HAELGN (55.82±0.35) and HAEBGN (62.38±0.45). The IC50 values (µg/ml) of different plant extracts inferred that DPPH radical scavenging activity is greater in HAELGN (42.91±0.88) and HAEBGN (53.87±0.35) than HAESGS (126.73±1.20) and HAERGS (88.87±1.25). However, hydroxyl and superoxide radical scavenging activity is more in HAERGS (135.41±1.19 & 88.00±1.42) and HAELGN (172.28±1.91 & 108.163±1.09) than HAESGS (237.3±1.65 & 110.074±0.87) and HAEBGN (204.7±1.04 & 125.54±1.07). The results of present comprehensive analysis demonstrated that both the plants Grewia serrulata DC and Grewia Nervosa (Lour.) panigrahi possess high phenolic, flavonoid contents and potential antioxidant activity, and could be used as a valid source of natural antioxidants and might be utilized for pharmacological screening of various therapeutic activities. Keywords: Grewia serrulata; Grewia Nervosa; Total Phenolic content; Total flavonoid content; Antioxidant potential

Sign in / Sign up

Export Citation Format

Share Document