total flavonoid content
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Author(s):  
Annisa Fatmawati ◽  
Depita Sucianingsih ◽  
Riza Kurniawati ◽  
Muhammad Abdurrahman

This research was conducted to identify simplicia microscopically, phytochemical screening and determination of total flavonoid content of extract and ethyl acetate fraction from Moringa (Moringa oleifera L.) leaves using UV-Visible Spectrophotometry method. The experimental design used in this study was to perform microscopic identification of Moringa leaf powder simplicia, make 96% and 70% ethanol extract and ethyl acetate fraction of Moringa leaves from 70% ethanol extract, then carry out phytochemical screening and determination of total flavonoid content with quercetin standards. Phytochemical screening on the ethyl acetate fraction of Moringa leaves included tests for the content of flavonoids, saponins, tannins and alkaloids. The results of microscopic identification of Moringa leaf simplicia showed the presence of calcium oxalate crystals in the form of rosettes, mesophyll and stomata. The result of determination of total flavonoid content in 96% ethanol extract was 16.69 ± 0.74% (w/w), 70% ethanol extract was 10.84 ± 0.49% (w/w), Moringa leaf ethyl acetate fraction 14 .45 ± 0.90% (w/w). The highest total flavonoid content was found in the 96% ethanol extract of Moringa leaves in accordance with the 2017 Indonesian Herbal Pharmacopoeia, that the thick extract of Moringa leaves containing no less than 6.30% (w/w) total flavonoids was calculated as quercetin.


2022 ◽  
Vol 956 (1) ◽  
pp. 012001
Author(s):  
N Nerdy ◽  
B R Barus ◽  
H J El-Matury ◽  
S Ginting ◽  
N F Zebua ◽  
...  

Abstract Free radicals can cause various degenerative diseases such as cancer and cardiovascular disease. Flavonoids have antioxidant abilities that inhibit damage from free radicals. The roselle antioxidant mechanism is associated with high flavonoid content. Red and purple roselles are two varieties of roselle (Hibiscus sabdariffa L.) cultivated in Indonesia. The aim of this research was to analyze the differences in flavonoid content and antioxidant activity in the calyces of two varieties of roselle (Hibiscus sabdariffa L.), red and purple. Total flavonoid analysis and antioxidant activity testing were done by spectrophotometry in the visible region. Total flavonoid content was analyzed by pretreatment with aluminium chloride and sodium acetate. Antioxidant activity was tested with 2,2-diphenyl-1-picrylhydrazyl. The total flavonoids for both red and purple roselle calyces ethanolic extract were 27.55 and 39.18 mg quercetin equivalent per gram. The half maximal inhibitory concentration values obtained for both red and purple roselle calyces ethanolic extract a were 51.59 and 42.17 μg/mL. The total flavonoid content and antioxidant activity of purple roselle calyces ethanolic extract was higher and stronger than red roselle calyces ethanolic extract.


2021 ◽  
Vol 19 (4) ◽  
pp. 693-703
Author(s):  
Ah-Ra Hwang ◽  
Myung-Ryun Han ◽  
Ae-Jung Kim

Purpose: We evaluated the physicochemical characteristics and antioxidant activity of 21 types of fruit tea extract that are consumed in Korea. The data were analyzed and curated for the development of processed products using tea in the future.Methods: After extraction using hot water at 70℃, we analyzed the physicochemical properties including color and pH, as well as the antioxidant activity (total flavonoid content, DPPH scavenging activity, and α-glucosidase inhibitory activity).Results: Calamansi tea exhibited the lowest brightness with a beet-red appearance, and a yellowness that was significantly higher in jujube and radish. The pH ranged from 3.04 to 7.18 and most samples were in an acidic state. Of these, the pH of schizandra fruit tea was the lowest at 3.04±0.01. The total flavonoid content was the highest in calamansi at 217.87±1.45 QE/g, whereas that of cacao nibs was the lowest at 29.04±0.70 QE/g. The α-glucosidase inhibitory activity of Chinese quince was the highest at 59.96%±0.22%.Conclusion: We obtained basic data for several fruit tea extracts by analyzing the physicochemical properties and antioxidant activities. We anticipate that these results will represent useful data for blending fruits for tea product development.


Author(s):  
Arky Jane Langstieh ◽  
Julie Birdie Wahlang ◽  
Clarissa Jane Lyngdoh ◽  
Ibaphylla Jaba ◽  
Chayna Sarkar ◽  
...  

Flavonoids are secondary plant metabolites normally found as pigmented compounds in plants. Quercetin and rutin are two important and commonly found flavonoids in nature and exhibit wide pharmacological effects such as antioxidant, anticarcinogenic, antiviral, anti-inflammatory, antidiabetic, and hepatoprotective activities as well as antimicrobial activity. In this study, quercetin and rutin content is being quantified in the plant extracts of Centella asiatica and Houttuynia cordata and considerable amounts of these two flavonoids were depicted. A single beam UV – Spectrophotometer was used to measure the absorbance of the standard as well as test solutions. Calibration curves were constructed for standard quercetin and rutin in such a way that x-axis denotes concentration and the y-axis denotes the absorbance. The calibration curves showed linearity at concentrations 5-25 ?g /ml of quercetin and rutin respectively with a good correlation coefficient (r) of 0.99 for both the curves. The absorbance of the two test extracts was obtained from the calibration curve and respective concentrations of quercetin and rutin for the two extracts were calculated. The amount of quercetin and rutin present was expressed as Total Flavonoid Content (TFC) i.e. the amount of the flavonoid in ?g present per mg of the respective plant extract. The quercetin content in both the plant extracts was found to be more (315.8 in Houttuynia cordata; 487.6 in Centella asiatica) than the content of rutin (152.2 in Houttuynia cordata; 171.0 in Centella asiatica).


Author(s):  
Dolly Rani ◽  
Harsha Kharkwal ◽  
Megha Jha ◽  
Nitin Rai

A variety of secondary metabolites provide a valuable source of treatment for various pharmacological ailments. Grewia abutilifolia is a shrub found in the family Tiliaceae that is also investigated for its nutritional and medicinal benefits. Grewia is reported for its good antioxidant potential, which is applied to protect skin from UV rays. The main goal of this study is to determine the in-vitro sunscreen effect of Grewia abutilifolia based on phytochemical evidence of UV protection. Chemical treatments used to protect skin from the sun have several drawbacks, including numerous hazardous side effects on humans and the environment. The hydroalcoholic, ethyl acetate, and methanolic extracts were studied for their phytochemical, physicochemical, and sun protection activities. Total phenolic content, total alkaloid content, total flavonoid content, and SPF value were all highest in the methanolic leaf extract, with values of 99.20 0.12, 47.661.527, 96.02, and 14.50.01 for total phenolic content, total alkaloid content, total flavonoid content, and SPF value, respectively.


2021 ◽  
Vol 913 (1) ◽  
pp. 012042
Author(s):  
N Ardiana ◽  
A S Abidin ◽  
B T K Ilhami ◽  
A L Sunarwidhi ◽  
S Widyastuti ◽  
...  

Abstract Seaweed is one of the marine-biota that is widely known for its uses, one of them is in the food functional. Functional food is a program that promotes a healthy diet so that it can prevent a disease when consuming food. With functional food, daily eating patterns can be maintained and also the content of the food consumed daily has a good effect on the body. It is known that in seaweed, especially brown algae (S. crassifolium), it contains antioxidants and other compounds that are good for the health. In this study, phytochemical content and proximate composition was evaluated in S. crassifolium. Including total flavonoid content and total phenolic content, antioxidant capacity both of DPPH and ABTS assay used three type of solvents such as Ethanol, n-Hexana, and ethyl acetate for comparable the best solvents. Also, proximate analysis to determine the content of moisture content, crude fat content, crude protein content, and carbohydrates content. The results showed that n-Hexana and ethyl acetate solvents were significantly than ethanol. Which mean both of them had highest values of total flavonoid content (TFC), total phenolic content (TPC), and antioxidant activity of DPPH radical scavenging activity and ABTS radical scavenging activity. The results of chemical composition in proximate compounds showed that S. crassifolium was suitable to be candidate of functional food.


2021 ◽  
Vol 913 (1) ◽  
pp. 012084
Author(s):  
B Prihardina ◽  
S Fatmawati

Abstract Begonia medicinalis or known as benalu batu in Indonesia is a herbal plant that is locally used for traditional medicines. The secondary metabolites such as flavonoids, alkaloids, steroids, and terpenoids have been reported to be found in these plant extracts. The content of flavonoids can lead to anti-cancer abilities while heat-sensitive flavonoid compounds can be extracted by the Ultrasound-assisted Extraction (UAE) method. In this study, the anticancer potential of B. medicinalis extracts from the leaves (leaves extract/LE) and stem (stem extract/SE) in three cell lines (Hela, MDA-MB, HT-29) have been performed. Extraction of the leaves and stems was carried out using water as a solvent and the ultrasound-assisted extraction (UAE) method followed by measuring the total flavonoid content (TFC) of each extract. The anticancer potential was obtained from cytotoxic measurements by the MTT method on 3 types of cancer cells incubated with the extract for 24 hours. The value of total flavonoid content (TFC) in the LE was higher than that of SE extracts. Both extracts have the potential as a remedy for the treatment of cancer. Keywords: Begonia medicinalis, Ultrasound-assisted Extraction (UAE), anticancer


2021 ◽  
Vol 913 (1) ◽  
pp. 012109
Author(s):  
A Rosyantari ◽  
ES Prasedya ◽  
BTK Ilhami ◽  
NWR Martyasari ◽  
H Padmi ◽  
...  

Abstract Marine sponge Stylissa flabelliformis is a marine sponge species that is widely found in Indonesia. Marine sponges have been proven to have pharmacological activities including wound healing, cytotoxicity and antimicrobial activity. All these activities are related to its antioxidant potential. Antioxidants are important for human health due to its ability to protect cell damage caused by free radicals. The aim of this research was to study the antioxidant activity of marine sponge (Stylissa flabelliformis) and to observe the phytochemical compound of the marine sponge such as the phenolic and flavonoid content. Extraction was performed using cold maceration with 96% ethanol solvent and continued by rotary evaporator to get the concentrated extract. Antioxidants activity was analysed using DPPH (2,2-diphenyl-1-picrylhydrazyl) method, which examines the inhibitory concentration (IC50) of the ethanol extract. Total phenolic content (TPC) was examined using Gallic acid as the standard, while total flavonoid content (TFC) was examined using quercetin as the standard. The result showed a concentration-dependent antiradical activity by reduction of DPPH with an IC50 value 2000 µg/mL. Total phenolic content was 152,67 mg GAE/g, while the total flavonoid content was 155,79 mg QE/g.


2021 ◽  
Vol 891 (1) ◽  
pp. 012017
Author(s):  
Masendra ◽  
A Ngadianto ◽  
R Arifriana ◽  
F Maulidia ◽  
W Minorita ◽  
...  

Abstract This study aimed to investigate the polyphenol contents from the Pinus merkusii cone extract and their antioxidant activity. The cone powder (40 mesh) was extracted with n-hexane, ethyl acetate, and methanol successively for six hours. The cone of n-hexane, ethyl acetate, and methanol soluble extracts was subjected to total tannin content, total phenol content, total flavonoid content, and antioxidant measurements. The polyphenol contents were measured through the colorimetric method, while antioxidant activity was observed by inhibition of the DPPH (1,1-diphenyl-2-picrylhydrazyl) method. The results showed that total tannin content, total phenol content, total flavonoid content, and antioxidant activity of methanol soluble extract was higher than ethyl acetate and n-hexane soluble extracts. In this study, the high antioxidant activity of methanol soluble extract (IC50 of 196.73±13.93 ppm) might due to the high concentration of total phenol content (545.38±54.07 mg GAE/g sample). It was suggested that the P. merkusii cone is potential as the antioxidant source.


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