Investigation by Long PCR of the Genetic Elements Mediating Vana Glycopeptide Resistance in Enterococci from Uncooked Meat in South Manchester

Streptococci and the Host - Advances in Experimental Medicine and Biology ◽

10.1007/978-1-4899-1825-3_98 ◽

1997 ◽

pp. 409-412 ◽

Cited By ~ 10

Author(s):

Neil Woodford ◽

Adrian P. Watson ◽

Paul R. Chadwick

Keyword(s):

Glycopeptide Resistance ◽

Genetic Elements ◽

Long Pcr

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Epidemiology of the Genetic Elements Responsible for Acquired Glycopeptide Resistance in Enterococci

Microbial Drug Resistance ◽

10.1089/10766290152652774 ◽

2001 ◽

Vol 7 (3) ◽

pp. 229-236 ◽

Cited By ~ 63

Author(s):

Neil Woodford

Keyword(s):

Glycopeptide Resistance ◽

Genetic Elements

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Identification and Characterization ofvanB2 Glycopeptide Resistance Elements in Enterococci Isolated in Scotland

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.44.9.2341-2348.2000 ◽

2000 ◽

Vol 44 (9) ◽

pp. 2341-2348 ◽

Cited By ~ 12

Author(s):

K. F. McGregor ◽

H.-K. Young

Keyword(s):

Restriction Fragment Length Polymorphism ◽

Fragment Length Polymorphism ◽

Polymorphism Analysis ◽

Strain Atcc ◽

Glycopeptide Resistance ◽

Nucleotide Level ◽

Long Pcr ◽

Restriction Sites ◽

Identification And Characterization ◽

Restriction Fragment Length

ABSTRACT Thirty-two vanB glycopeptide-resistant enterococci (28Enterococcus faecium and 4 Enterococcus faecalis) were collected from hospitalized patients in Glasgow, Edinburgh, Dundee, and Aberdeen, Scotland, and the vanBelement in each was compared to vanB1 of E. faecalis strain ATCC 51299. HhaI digestion of PCR fragments of the vanB ligase gene was used to identifyvanB subtypes. All E. faecium isolates werevanB2, and all E. faecalis isolates werevanB1. Restriction fragment length polymorphism analysis of a 5,180-bp vanSB-vanXB long-PCR fragment of the vanB cluster showed the loss ofHaeII restriction sites in vanSB ,vanW, and vanXB in strains containing a vanB2 ligase gene. Partial sequences of genes in the vanB2 cluster for two genomically distinct Scottish isolates were >99.8% identical to each other.vanSB2 , vanXB2 , andvanB2 sequences differed at the nucleotide level from those of vanSB , vanXB , andvanB by 4.2, 4.6, and 4.8%, respectively. ThevanB2 resistance element appears to be widespread among VanB glycopeptide-resistant E. faecium strains isolated in Scottish hospitals.

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