scholarly journals Real-Time, In Vivo Determination of Dynamic Changes in Lung and Heart Tissue Oxygenation Using EPR Oximetry

2014 ◽  
pp. 81-86 ◽  
Author(s):  
Brian K. Rivera ◽  
Shan K. Naidu ◽  
Kamal Subramanian ◽  
Matthew Joseph ◽  
Huagang Hou ◽  
...  
Keyword(s):  
Real Time ◽  
Tissue Oxygenation ◽  
Heart Tissue ◽  
Dynamic Changes ◽  
Epr Oximetry

Abstract 352: Real-time Intravital Optical Imaging Reflects the Dynamic Changes of Oxidative Stress Induced by Cigarette Smoking in Vasculatures

2016 ◽  
Vol 36 (suppl_1) ◽  
Author(s):  
Ji Bak Kim ◽  
Jiheun Ryu ◽  
Joon Woo Song ◽  
Dong Joo Oh ◽  
DaeGab Gweon ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cigarette Smoking ◽  
Optical Imaging ◽  
Real Time ◽  
Treated Group ◽  
Fluorescence Signal ◽  
Ros Production ◽  
Protective Effects ◽  
Dynamic Changes

Background: Reactive oxygen species (ROS) play a central role in cigarette smoking-induced atherogenesis. The present study aims to assess the smoking-induced acute oxidative stress within vasculatures, and evaluates whether the resveratrol, a natural polyphenol antioxidant, can counteract this ROS production, using a customized, high resolution intravital optical imaging in real-time. Methods and Results: 20-week-old male C57BL/6 mice were divided into four groups according to the preceding administration of resveratrol (R) (25mg/kg via gavage, for 7 days) and exposure to cigarette smoke (CS). To in vivo assess acute oxidative stress in blood vessels, dihydroethidium, which forms a red fluorescence (ethidium, excitation/emission: 520nm/610nm) upon reaction with ROS, was injected intraperitoneally. During CS exposure, temporal changes of fluorescence signals from the mouse cremaster muscle including vasculatures were assessed by intravital optical imaging for 15 minutes. Fluorescence signals were much more pronounced in CS exposed mice than controls (p<0.001). Resveratrol p.o. significantly reduced the CS-induced ROS signals compared to the non-treated group (fluorescence signal to noise ratio, SNR, 2.51±0.09 vs. 12.52±2.116, p=0.0002) (Figure A). Without CS exposure, fluorescence signals in targeted vasculatures were very low showing no difference between groups (SNR, 1.65±0.19 vs. 1.53±0.07, p=0.80) (Figure A). Lipid peroxidation was increased in CS group and significantly attenuated in resveratrol-treated mice (Figure B). Fluorescence microscopy and immunostainings corroborated the in vivo findings. Conclusions: The intravital optical imaging was able to in vivo estimate the dynamic changes of ROS production by CS exposure. Our data demonstrated that even a brief exposure to CS increased oxidative stress in vasculatures promptly, and the resveratrol exerts protective effects against the CS-induced acute oxidative stress.

scholarly journals In vivo evidence of methamphetamine induced attenuation of brain tissue oxygenation as measured by EPR oximetry

2014 ◽  
Vol 275 (2) ◽  
pp. 73-78 ◽  
Author(s):  
John Weaver ◽  
Yirong Yang ◽  
Rebecca Purvis ◽  
Theodore Weatherwax ◽  
Gerald M. Rosen ◽  
...  
Keyword(s):  
Brain Tissue ◽  
Tissue Oxygenation ◽  
Brain Tissue Oxygenation ◽  
Epr Oximetry

scholarly journals In Vivo Determination of Renal Tissue Oxygenation During Pulsatile and Nonpulsatile Left Heart Bypass

1973 ◽  
Vol 15 (4) ◽  
pp. 354-363 ◽  
Author(s):  
N.D. Mukherjee ◽  
A.V. Beran ◽  
J. Hirai ◽  
A. Wakabayashi ◽  
D.R. Sperling ◽  
...  
Keyword(s):  
Tissue Oxygenation ◽  
Renal Tissue ◽  
Left Heart ◽  
Left Heart Bypass ◽  
Nonpulsatile Left Heart Bypass ◽  
Heart Bypass

Determination of Real Time in Vivo Drug Receptor Occupancy for a Covalent Binding Drug as a Clinical Pharmacodynamic Biomarker by Immunocapture-LC-MS/MS

2019 ◽  
Vol 91 (13) ◽  
pp. 8443-8452 ◽  
Author(s):  
Naiyu Zheng ◽  
Ian M. Catlett ◽  
Kristin Taylor ◽  
Huidong Gu ◽  
Mark A. Pattoli ◽  
...  
Keyword(s):  
Real Time ◽  
Covalent Binding ◽  
Receptor Occupancy ◽  
Drug Receptor

scholarly journals Use of 3-acetoxymethoxycarbonyl-2,2,5,5-tetramethyl-1-pyrrolidinyloxyl as an EPR oximetry probe: Potential for in vivo measurement of tissue oxygenation in mouse brain

2006 ◽  
Vol 55 (6) ◽  
pp. 1433-1440 ◽  
Author(s):  
Jiangang Shen ◽  
Shimin Liu ◽  
Minoru Miyake ◽  
Wenlan Liu ◽  
Aaron Pritchard ◽  
...  
Keyword(s):  
Mouse Brain ◽  
Tissue Oxygenation ◽  
In Vivo Measurement ◽  
Epr Oximetry ◽  
Oximetry Probe ◽  
Probe Potential

A ratiometric photoacoustic imaging approach for semi-quantitative determination of aggregation efficiency in vivo

Nanoscale ◽  
2020 ◽  
Vol 12 (36) ◽  
pp. 18654-18662
Author(s):  
Bo Peng ◽  
Xiu-Mei Liu ◽  
Hsian-Rong Tseng ◽  
Li-Li Li ◽  
Hao Wang
Keyword(s):  
Quantitative Determination ◽  
Real Time ◽  
Pancreatic Tumor ◽  
Photoacoustic Imaging ◽  
High Contrast ◽  
Imaging Approach

We first proposed a ratiometric photoacoustic (PA) imaging approach for real-time semi-quantitative calculated aggregation efficiency in vivo. The designed probe with high contrast will used for pancreatic tumor bioimaging or operation navigation.

Determination of real-time in-vivo cartilage contact deformation in the ankle joint

2008 ◽  
Vol 41 (1) ◽  
pp. 128-136 ◽  
Author(s):  
Guoan Li ◽  
Lu Wan ◽  
Michal Kozanek
Keyword(s):  
Real Time ◽  
Ankle Joint ◽  
Contact Deformation

scholarly journals EPR oximetry as a method for repetitive measurements of in vivo kidney tissue oxygenation (890.2)

2014 ◽  
Vol 28 (S1) ◽  
Author(s):  
Stephanie Franzén ◽  
Liselotte Pihl ◽  
Nadeem Khan ◽  
Håkan Gustafsson ◽  
Fredrik Palm
Keyword(s):  
Tissue Oxygenation ◽  
Kidney Tissue ◽  
Epr Oximetry
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