Bacterial Expression and Stabilization of GPCRs

2014 ◽  
pp. 71-86
Author(s):  
Jean-Louis Banères
Keyword(s):  
Bacterial Expression

Conditions Resulting in Formation of Properly Assembled Retroviral Capsids within Inclusion Bodies of Escherichia coli

1999 ◽  
Vol 64 (8) ◽  
pp. 1348-1356 ◽  
Author(s):  
Michaela Rumlová-Kliková ◽  
Iva Pichová ◽  
Eric Hunter ◽  
Tomáš Ruml
Keyword(s):  
Escherichia Coli ◽  
Inclusion Bodies ◽  
Amorphous Material ◽  
Bacterial Expression ◽  
Biologically Active ◽  
Capsid Assembly ◽  
Cultivation Medium ◽  
Viral Particles ◽  
Induction Temperature

It has been generally accepted that inclusion bodies (IBs) formed in Escherichia coli consist of non-biologically active aggregated proteins, which are stabilized by non-productive interactions. We show here that bacterial expression of a retroviral capsid polyprotein results in formation of insoluble IBs that contain fully assembled viral particles connected with amorphous material. The efficiency of IBs formation and capsid assembly was not significantly affected by changes in induction temperature, pH of cultivation medium or the level of expression.

Improved soluble bacterial expression and properties of the recombinant flavonoid glucosyltransferase UGT73G1 from Allium cepa

2017 ◽  
Vol 255 ◽  
pp. 9-15 ◽  
Author(s):  
Ruxin Cai ◽  
Caihong Chen ◽  
Yan Li ◽  
Kaiyan Sun ◽  
Fangfang Zhou ◽  
...  
Keyword(s):  
Allium Cepa ◽  
Bacterial Expression

Comparative Analysis of Bacterial Expression Systems for Keratinase Production

2014 ◽  
Vol 173 (5) ◽  
pp. 1222-1235 ◽  
Author(s):  
Baihong Liu ◽  
Juan Zhang ◽  
Lei Gu ◽  
Guocheng Du ◽  
Jian Chen ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Bacterial Expression ◽  
Expression Systems ◽  
Bacterial Expression Systems

Bacterial expression of immunoglobulin VH proteins

1990 ◽  
Vol 27 (1) ◽  
pp. 25-35 ◽  
Author(s):  
Keiko Udaka ◽  
Chua Ming-Ming ◽  
Tong Li-Heng ◽  
Fred Karush ◽  
Sol H. Goodgal
Keyword(s):  
Bacterial Expression

scholarly journals Bacterial expression and conformational analysis of a chemosensory protein from Schistocerca gregaria

2001 ◽  
Vol 268 (17) ◽  
pp. 4794-4801 ◽  
Author(s):  
Delia Picone ◽  
Orlando Crescenzi ◽  
Sergio Angeli ◽  
Silvana Marchese ◽  
Anna Brandazza ◽  
...  
Keyword(s):  
Conformational Analysis ◽  
Schistocerca Gregaria ◽  
Bacterial Expression ◽  
Chemosensory Protein
Sign in / Sign up

Export Citation Format

Share Document