Membrane Protein Production in Escherichia coli: Overview and Protocols

2014 ◽  
pp. 87-106 ◽  
Author(s):  
Georges Hattab ◽  
Annabelle Y. T. Suisse ◽  
Oana Ilioaia ◽  
Marina Casiraghi ◽  
Manuela Dezi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Membrane Protein ◽  
Protein Production

Development of Escherichia coli Strains That Withstand Membrane Protein-Induced Toxicity and Achieve High-Level Recombinant Membrane Protein Production

2016 ◽  
Vol 6 (2) ◽  
pp. 284-300 ◽  
Author(s):  
Dimitra Gialama ◽  
Kalliopi Kostelidou ◽  
Myrsini Michou ◽  
Dafni Chrysanthi Delivoria ◽  
Fragiskos N. Kolisis ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Membrane Protein ◽  
Protein Production ◽  
High Level

Shaping Escherichia coli for recombinant membrane protein production

2018 ◽  
Vol 365 (15) ◽  
Author(s):  
Alexandros Karyolaimos ◽  
Henry Ampah-Korsah ◽  
Zhe Zhang ◽  
Jan-Willem de Gier
Keyword(s):  
Escherichia Coli ◽  
Membrane Protein ◽  
Protein Production

Functional Requirements for DjlA- and RraA-Mediated Enhancement of Recombinant Membrane Protein Production in the Engineered Escherichia coli Strains SuptoxD and SuptoxR

2017 ◽  
Vol 429 (12) ◽  
pp. 1800-1816 ◽  
Author(s):  
Dimitra Gialama ◽  
Dafni Chrysanthi Delivoria ◽  
Myrsini Michou ◽  
Artemis Giannakopoulou ◽  
Georgios Skretas
Keyword(s):  
Escherichia Coli ◽  
Membrane Protein ◽  
Protein Production ◽  
Functional Requirements ◽  
Engineered Escherichia Coli

scholarly journals Simple Genetic Selection Protocol for Isolation of Overexpressed Genes That Enhance Accumulation of Membrane-Integrated Human G Protein-Coupled Receptors in Escherichia coli

2010 ◽  
Vol 76 (17) ◽  
pp. 5852-5859 ◽  
Author(s):  
Georgios Skretas ◽  
George Georgiou
Keyword(s):  
Escherichia Coli ◽  
Membrane Proteins ◽  
Membrane Protein ◽  
G Protein ◽  
Protein Production ◽  
Genetic Selection ◽  
G Protein Coupled Receptors ◽  
Efficient Production ◽  
E Coli ◽  
G Protein Coupled

ABSTRACT The efficient production of membrane proteins in bacteria remains a major challenge. In this work, we sought to identify overexpressed genes that enhance the yields of recombinant membrane proteins in Escherichia coli. We developed a genetic selection system for bacterial membrane protein production, consisting of membrane protein fusions with the enzyme β-lactamase and facile selection of high-production strains on ampicillin-containing media. This system was used to screen the ASKA library, an ordered library of plasmids encoding all the known E. coli open reading frames (ORFs), and several clones with the ability to accumulate enhanced amounts of recombinant membrane proteins were selected. Notably, coexpression of ybaB, a gene encoding a putative DNA-binding protein of unknown function, was found to enhance the accumulation of a variety of membrane-integrated human G protein-coupled receptors and other integral membrane proteins in E. coli by up to 10-fold. The results of this study highlight the power of genetic approaches for identifying factors that impact membrane protein biogenesis and for generating engineered microbial hosts for membrane protein production.

scholarly journals Bicistronic Design-Based Continuous and High-Level Membrane Protein Production in Escherichia coli

2019 ◽  
Vol 8 (7) ◽  
pp. 1685-1690 ◽  
Author(s):  
Nico J. Claassens ◽  
Max Finger-Bou ◽  
Bart Scholten ◽  
Frederieke Muis ◽  
Jonas J. de Groot ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Membrane Protein ◽  
Protein Production ◽  
High Level

scholarly journals Optimization of Recombinant Membrane Protein Production in the Engineered Escherichia coli Strains SuptoxD and SuptoxR

2019 ◽  
Vol 8 (7) ◽  
pp. 1631-1641 ◽  
Author(s):  
Myrsini Michou ◽  
Charalampos Kapsalis ◽  
Christos Pliotas ◽  
Georgios Skretas
Keyword(s):  
Escherichia Coli ◽  
Membrane Protein ◽  
Protein Production ◽  
Engineered Escherichia Coli
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