Isolation of Adult Stem Cell Populations from the Human Cornea

2014 ◽  
pp. 165-177
Author(s):  
Matthew J. Branch ◽  
Wing-Yan Yu ◽  
Carl Sheridan ◽  
Andrew Hopkinson
Keyword(s):  
Stem Cell ◽  
Adult Stem Cell ◽  
Cell Populations ◽  
Human Cornea ◽  
Stem Cell Populations

scholarly journals The Adipose-derived Stem Cell: Looking Back and Looking Ahead

2010 ◽  
Vol 21 (11) ◽  
pp. 1783-1787 ◽  
Author(s):  
Patricia A. Zuk
Keyword(s):  
Stem Cell ◽  
Historical Perspective ◽  
Es Cells ◽  
Adult Stem Cell ◽  
Stem Cell Population ◽  
Cell Populations ◽  
Stem Cell Populations ◽  
New Applications ◽  
Adipose Derived Stem Cell ◽  
Looking Back

In 2002, researchers at UCLA published a manuscript in Molecular Biology of the Cell describing a novel adult stem cell population isolated from adipose tissue—the adipose-derived stem cell (ASC). Since that time, the ASC has gone on to be one of the most popular adult stem cell populations currently being used in the stem cell field. With multilineage mesodermal potential and possible ectodermal and endodermal potentials also, the ASC could conceivably be an alternate to pluripotent ES cells in both the lab and in the clinic. In this retrospective article, a historical perspective on the ASC is given together with exciting new applications for the stem cell being considered today.

The Lineage Before Time: Circadian and Nonclassical Clock Influences on Development

2020 ◽  
Vol 36 (1) ◽  
pp. 469-509
Author(s):  
Joseph Lewis Bedont ◽  
Daniel Maxim Iascone ◽  
Amita Sehgal
Keyword(s):  
Stem Cell ◽  
Chromatin Remodeling ◽  
Feedback Loop ◽  
Adult Stem Cell ◽  
Cellular Level ◽  
Cell Populations ◽  
Pluripotent Cells ◽  
Cellular Identity ◽  
Stem Cell Populations ◽  
Early Developmental

Diverse factors including metabolism, chromatin remodeling, and mitotic kinetics influence development at the cellular level. These factors are well known to interact with the circadian transcriptional-translational feedback loop (TTFL) after its emergence. What is only recently becoming clear, however, is how metabolism, mitosis, and epigenetics may become organized in a coordinated cyclical precursor signaling module in pluripotent cells prior to the onset of TTFL cycling. We propose that both the precursor module and the TTFL module constrain cellular identity when they are active during development, and that the emergence of these modules themselves is a key lineage marker. Here we review the component pathways underlying these ideas; how proliferation, specification, and differentiation decisions in both developmental and adult stem cell populations are or are not regulated by the classical TTFL; and emerging evidence that we propose implies a primordial clock that precedes the classical TTFL and influences early developmental decisions.

scholarly journals Characterization of human adult stem‐cell populations isolated from visceral and subcutaneous adipose tissue

2009 ◽  
Vol 23 (10) ◽  
pp. 3494-3505 ◽  
Author(s):  
Silvana Baglioni ◽  
Michela Francalanci ◽  
Roberta Squecco ◽  
Adriana Lombardi ◽  
Giulia Cantini ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Stem Cell ◽  
Subcutaneous Adipose Tissue ◽  
Adult Stem Cell ◽  
Cell Populations ◽  
Human Adult ◽  
Stem Cell Populations ◽  
Subcutaneous Adipose

scholarly journals Nuclear factor one transcription factors: Divergent functions in developmental versus adult stem cell populations

2014 ◽  
Vol 244 (3) ◽  
pp. 227-238 ◽  
Author(s):  
Lachlan Harris ◽  
Laura A. Genovesi ◽  
Richard M. Gronostajski ◽  
Brandon J. Wainwright ◽  
Michael Piper
Keyword(s):  
Stem Cell ◽  
Transcription Factors ◽  
Adult Stem Cell ◽  
Cell Populations ◽  
Nuclear Factor ◽  
Stem Cell Populations

scholarly journals Prom1 expression does not mark a stem/progenitor population in the mouse oviduct epithelium

2020 ◽  
Author(s):  
Matthew J Ford ◽  
Yojiro Yamanaka
Keyword(s):  
Stem Cell ◽  
Adult Stem Cell ◽  
Lineage Tracing ◽  
Stem Cell Marker ◽  
Secretory Cells ◽  
Cell Populations ◽  
Cell Marker ◽  
Multiciliated Cells ◽  
Cell Property ◽  
Stem Cell Populations

SummaryThe oviduct or fallopian tube is the site of fertilization and preimplantation embryonic development. The epithelium lining the oviduct consists of multiciliated and secretory cells, which support fertilization and preimplantation development, however, its homeostasis still remains poorly understood. CD133/Prom1 has been used to identify adult stem cell populations in various organs and often associated with cancer stem cell property. Using a Cre-recombinase based lineage tracing strategy, we found that CD133/Prom1 expression was not associated with a stem/progenitor population in the oviduct but marked a sub population of multiciliated and secretory cells which did not propagate. Interestingly, Prom1 expressing secretory cells rapidly transition to multiciliated cells and progressively migrate to the tips of epithelial folds in the ampulla. Our results show that CD133/Prom1 expression cannot be used as a progenitor/stem cell marker in the mouse oviduct.

scholarly journals The impact of housing conditions on porcine adult stem cell populations differ between adipose tissue and skeletal muscle

2021 ◽  
Author(s):  
Audrey QUEMENER ◽  
Frederic DESSAUGE ◽  
Marie-Helene PERRUCHOT ◽  
Nathalie LE FLOC'H ◽  
Isabelle LOUVEAU
Keyword(s):  
Skeletal Muscle ◽  
Stem Cells ◽  
Adipose Tissue ◽  
Stem Cell ◽  
Adult Stem Cells ◽  
Adult Stem Cell ◽  
Cell Populations ◽  
Housing Conditions ◽  
Stem Cell Populations ◽  
The Impact

Background: In pigs, the ratio between lean mass and fat mass determines production efficiency and is strongly influenced by the number and size of cells in tissues. During growth, the increase in the number of cells results from the recruitment of different populations of multipotent adult stem cells residing in the tissues. We hypothesized that the impact of a hygiene challenge on the proportions of adult stem cells in adipose tissue and skeletal muscle may differ between pigs with different residual feed intake (RFI), a measure of feed efficiency. Methods: At the age of 11 weeks, Large White pigs from two lines divergently selected for low and high RFI were housed in two contrasting hygiene conditions (good vs poor). After six weeks of challenge, pigs were slaughtered (n = 5-9/group). Samples of subcutaneous adipose tissue and longissimus muscle were collected, and cells from the stromal vascular fraction (FSV), which includes adult stem cell populations, were isolated from each tissue. Adipose and muscle cell populations from the FSV were phenotyped by flow cytometry using antibodies that targeted different cell surface markers (CD45 to identify hematopoietic cells; CD34, CD38, CD56 and CD140a to identify mesenchymal stem cells (MSC) with adipogenic and/or myogenic potential). Results: Adipose tissue and muscle shared some common MSC populations although MSC diversity was higher in muscle than in adipose tissue. In muscle, the CD45-CD56+CD34-CD140a+ and CD45-CD56+CD34+CD140a+ cell populations were abundant. Of these two cell populations, only the proportions of CD45-CD56+CD34+CD140a+ cells increased (P < 0.05) in pigs housed in poor hygiene compared with good hygiene conditions. For the CD45-CD56-CD34- cell population, present in low proportion, there was an interaction between hygiene condition and genetic line (P < 0.05) with a decrease in low RFI pigs housed in poor hygiene conditions. In adipose tissue, the two abundant MSC populations were CD45-CD56-CD34- and CD45-CD56+CD34-. The proportion of CD45-CD56-CD34- cells increased (P < 0.05) whereas the proportion of CD45-CD56+CD34- tended to decrease (P < 0.1) in pigs housed in poor conditions. This study shows that the proportions of some MSC populations were affected by hygiene of housing conditions in a tissue-dependent manner in pigs of both RFI lines. Therefore, these cell populations could be targeted to modulate growth and body composition in growing animals.

Skin: a promising reservoir for adult stem cell populations

2004 ◽  
Vol 62 (5) ◽  
pp. 683-688 ◽  
Author(s):  
Shi Chunmeng ◽  
Cheng Tianmin
Keyword(s):  
Stem Cell ◽  
Adult Stem Cell ◽  
Cell Populations ◽  
Stem Cell Populations

scholarly journals Tissue-Resident Adult Stem Cell Populations of Rapidly Self-Renewing Organs

2010 ◽  
Vol 7 (6) ◽  
pp. 656-670 ◽  
Author(s):  
Nick Barker ◽  
Sina Bartfeld ◽  
Hans Clevers
Keyword(s):  
Stem Cell ◽  
Adult Stem Cell ◽  
Cell Populations ◽  
Stem Cell Populations
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