Transglutaminase-Mediated In Situ Hybridization (TransISH) for mRNA Detection in Mammalian Tissues

2015 ◽  
pp. 549-558
Author(s):  
Katsuyuki Miyawaki ◽  
Sumihare Noji ◽  
Noriho Kamiya
Keyword(s):  
In Situ Hybridization ◽  
Mrna Detection ◽  
Mammalian Tissues

scholarly journals Freeze-drying Allows Double Nonradioactive ISH and Antigenic Labeling

2000 ◽  
Vol 48 (4) ◽  
pp. 499-507 ◽  
Author(s):  
Huguette Louis ◽  
Julie Lavie ◽  
Patrick Lacolley ◽  
Danièle Daret ◽  
Jacques Bonnet ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Freeze Drying ◽  
Simultaneous Detection ◽  
Tissue Preservation ◽  
Reliable Technique ◽  
Mrna Detection ◽  
Nonradioactive In Situ Hybridization

Because tissue freeze-drying is an excellent way to preserve antigenic conformation, we have tested the feasibility of this technique to reveal nonradioactive in situ hybridization (ISH) of tissue mRNA. We have compared mRNA detection after different methods of tissue preservation, freeze-drying, cryosectioning, and formaldehyde or methanol fixation. Our results show that nonradioactive ISH is more sensitive for tissues preserved by freeze-drying than for other tissue preparations. We have demonstrated that freeze-drying allows combination of ISH and immunohistochemistry for simultaneous detection of mRNA and antigen because with this technique of tissue preservation ISH does not affect the sensitivity or the amount of the detected antigens. This work underscores the fact that tissue freeze-drying is an easy, convenient, and reliable technique for both ISH and immunohistochemistry and achieves excellent structural conditions for nonradioactive detection.

In situ hybridization for mRNA detection in Arabidopsis tissue sections

2006 ◽  
Vol 1 (3) ◽  
pp. 1462-1467 ◽  
Author(s):  
Philip B Brewer ◽  
Marcus G Heisler ◽  
Jan Hejátko ◽  
Jiří Friml ◽  
Eva Benková
Keyword(s):  
In Situ Hybridization ◽  
Mrna Detection ◽  
Tissue Sections

GAP-43 mRNA detection by in situ hybridization, direct and indirect in situ RT-PCR in hippocampal and cerebellar tissue sections of adult rat brain

Micron ◽  
2003 ◽  
Vol 34 (8) ◽  
pp. 415-422 ◽  
Author(s):  
Tiziana Casoli ◽  
Giuseppina Di Stefano ◽  
Patrizia Fattoretti ◽  
Moreno Solazzi ◽  
Alessia Delfino ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Rat Brain ◽  
Rt Pcr ◽  
Mrna Detection ◽  
Adult Rat ◽  
Tissue Sections ◽  
Adult Rat Brain ◽  
Cerebellar Tissue

scholarly journals Platelet-derived growth factor mRNA detection in human atherosclerotic plaques by in situ hybridization.

1988 ◽  
Vol 82 (3) ◽  
pp. 1134-1143 ◽  
Author(s):  
J N Wilcox ◽  
K M Smith ◽  
L T Williams ◽  
S M Schwartz ◽  
D Gordon
Keyword(s):  
In Situ Hybridization ◽  
Growth Factor ◽  
Platelet Derived Growth Factor ◽  
Atherosclerotic Plaques ◽  
Mrna Detection

scholarly journals Combined microRNA and mRNA detection in mammalian retinas by in situ hybridization chain reaction

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Pei Zhuang ◽  
Huanqing Zhang ◽  
Ryan M. Welchko ◽  
Robert C. Thompson ◽  
Shunbin Xu ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Hybridization Chain Reaction ◽  
Chain Reaction ◽  
Mrna Detection

scholarly journals Sensitive mRNA Detection by Fluorescence In Situ Hybridization Using Horseradish Peroxidase-labeled Oligodeoxynucleotides and Tyramide Signal Amplification

1998 ◽  
Vol 46 (11) ◽  
pp. 1249-1259 ◽  
Author(s):  
Mariëtte P.C. van de Corput ◽  
Roeland W. Dirks ◽  
Rob P.M. van Gijlswijk ◽  
Erica van Binnendijk ◽  
Claudia M. Hattinger ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Horseradish Peroxidase ◽  
Fluorescence In Situ Hybridization ◽  
Signal Amplification ◽  
Tyramide Signal Amplification ◽  
Mrna Detection
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