In Situ Hybridization to Embryo Whole Mounts and Tissue Sections: mRNA Detection and Application to Developmental Studies

2008 ◽  
pp. 91-121 ◽  
Author(s):  
T. Jowett ◽  
M. Mancera ◽  
A. Amores ◽  
Y-L. Yan
Keyword(s):  
In Situ Hybridization ◽  
Developmental Studies ◽  
Mrna Detection ◽  
Tissue Sections ◽  
Whole Mounts

In situ hybridization for mRNA detection in Arabidopsis tissue sections

2006 ◽  
Vol 1 (3) ◽  
pp. 1462-1467 ◽  
Author(s):  
Philip B Brewer ◽  
Marcus G Heisler ◽  
Jan Hejátko ◽  
Jiří Friml ◽  
Eva Benková
Keyword(s):  
In Situ Hybridization ◽  
Mrna Detection ◽  
Tissue Sections

GAP-43 mRNA detection by in situ hybridization, direct and indirect in situ RT-PCR in hippocampal and cerebellar tissue sections of adult rat brain

Micron ◽  
2003 ◽  
Vol 34 (8) ◽  
pp. 415-422 ◽  
Author(s):  
Tiziana Casoli ◽  
Giuseppina Di Stefano ◽  
Patrizia Fattoretti ◽  
Moreno Solazzi ◽  
Alessia Delfino ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Rat Brain ◽  
Rt Pcr ◽  
Mrna Detection ◽  
Adult Rat ◽  
Tissue Sections ◽  
Adult Rat Brain ◽  
Cerebellar Tissue

Application of in situ hybridization with a novel phenytoin-labeled probe to conventional formalin-fixed, paraffin-embedded tissue sections

1995 ◽  
Vol 52 (3) ◽  
pp. 309-316 ◽  
Author(s):  
Yoshito Eizuru ◽  
Yoichi Minamishima ◽  
Tadashi Matsumoto ◽  
Toshinari Hamakado ◽  
Mikio Mizukoshi ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Tissue Sections ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Labeled Probe ◽  
Formalin Fixed

scholarly journals Freeze-drying Allows Double Nonradioactive ISH and Antigenic Labeling

2000 ◽  
Vol 48 (4) ◽  
pp. 499-507 ◽  
Author(s):  
Huguette Louis ◽  
Julie Lavie ◽  
Patrick Lacolley ◽  
Danièle Daret ◽  
Jacques Bonnet ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Freeze Drying ◽  
Simultaneous Detection ◽  
Tissue Preservation ◽  
Reliable Technique ◽  
Mrna Detection ◽  
Nonradioactive In Situ Hybridization

Because tissue freeze-drying is an excellent way to preserve antigenic conformation, we have tested the feasibility of this technique to reveal nonradioactive in situ hybridization (ISH) of tissue mRNA. We have compared mRNA detection after different methods of tissue preservation, freeze-drying, cryosectioning, and formaldehyde or methanol fixation. Our results show that nonradioactive ISH is more sensitive for tissues preserved by freeze-drying than for other tissue preparations. We have demonstrated that freeze-drying allows combination of ISH and immunohistochemistry for simultaneous detection of mRNA and antigen because with this technique of tissue preservation ISH does not affect the sensitivity or the amount of the detected antigens. This work underscores the fact that tissue freeze-drying is an easy, convenient, and reliable technique for both ISH and immunohistochemistry and achieves excellent structural conditions for nonradioactive detection.

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