Companion Protease Inhibitors for the In Situ Protection of Recombinant Proteins in Plants

2016 ◽  
pp. 115-126 ◽  
Author(s):  
Stéphanie Robert ◽  
Philippe V. Jutras ◽  
Moustafa Khalf ◽  
Marc-André D’Aoust ◽  
Marie-Claire Goulet ◽  
...  
Keyword(s):  
Protease Inhibitors ◽  
Recombinant Proteins

SEM of non-coated hepatocellular cytoskeleton of perfused livers

1984 ◽  
Vol 42 ◽  
pp. 306-307
Author(s):  
S.W. French ◽  
N.C. Benson ◽  
C. Davis-Scibienski
Keyword(s):  
Ambient Temperature ◽  
Critical Point ◽  
Protease Inhibitors ◽  
Metal Deposition ◽  
Heat Damage ◽  
Detergent Extraction ◽  
Cytoskeletal Elements ◽  
Triton X ◽  
Excised Tissue

Previous SEM studies of liver cytoskeletal elements have encountered technical difficulties such as variable metal coating and heat damage which occurs during metal deposition. The majority of studies involving evaluation of the cell cytoskeleton have been limited to cells which could be isolated, maintained in culture as a monolayer and thus easily extracted. Detergent extraction of excised tissue by immersion has often been unsatisfactory beyond the depth of several cells. These disadvantages have been avoided in the present study. Whole C3H mouse livers were perfused in situ with 0.5% Triton X-100 in a modified Jahn's buffer including protease inhibitors. Perfusion was continued for 1 to 2 hours at ambient temperature. The liver was then perfused with a 2% buffered gluteraldehyde solution. Liver samples including spontaneous tumors were then maintained in buffered gluteraldehyde for 2 hours. Samples were processed for SEM and TEM using the modified thicarbohydrazide procedure of Malich and Wilson, cryofractured, and critical point dried (CPD). Some samples were mechanically fractured after CPD.

Protease Inhibitors Formed In Situ from Copper and Tridentate Chelates: A Generalized Approach towards Metal-Based Pharmaceuticals

ChemBioChem ◽  
2002 ◽  
Vol 3 (11) ◽  
pp. 1141-1143 ◽  
Author(s):  
Christopher J. Brinkerhoff ◽  
Paul Podsiadlo ◽  
Tomoko Komiyama ◽  
Robert S. Fuller ◽  
Ofer Blum
Keyword(s):  
Protease Inhibitors

Inhibition of post-mortem muscle softening following in situ perfusion of protease inhibitors in tilapia

2003 ◽  
Vol 69 (3) ◽  
pp. 632-638 ◽  
Author(s):  
Noriko ISHIDA ◽  
Michiaki YAMASHITA ◽  
Naoko KOIZUMI ◽  
Makoto TERAYAMA ◽  
Toshinao INENO ◽  
...  
Keyword(s):  
Protease Inhibitors ◽  
Post Mortem ◽  
In Situ Perfusion

Cell-free synthesis and in situ isolation of recombinant proteins

2006 ◽  
Vol 45 (2) ◽  
pp. 249-254 ◽  
Author(s):  
Tae-Wan Kim ◽  
In-Seok Oh ◽  
Jin-Ho Ahn ◽  
Cha-Yong Choi ◽  
Dong-Myung Kim
Keyword(s):  
Recombinant Proteins

Liquid Marbles as an Easy‐to‐Handle Compartment for Cell‐Free Synthesis and In Situ Immobilization of Recombinant Proteins

2018 ◽  
Vol 13 (12) ◽  
pp. 1800085 ◽  
Author(s):  
Noriho Kamiya ◽  
Yuki Ohama ◽  
Kosuke Minamihata ◽  
Rie Wakabayashi ◽  
Masahiro Goto
Keyword(s):  
Recombinant Proteins ◽  
In Situ Immobilization ◽  
Liquid Marbles

Comparative Effects on Intestinal Absorption in Situ by P-glycoprotein-Modifying HIV Protease Inhibitors

2004 ◽  
Vol 21 (10) ◽  
pp. 1862-1866 ◽  
Author(s):  
Martin Richter ◽  
Nóra Gyémánt ◽  
Joséf Molnár ◽  
Andreas Hilgeroth
Keyword(s):  
Intestinal Absorption ◽  
Protease Inhibitors ◽  
Hiv Protease ◽  
Hiv Protease Inhibitors ◽  
P Glycoprotein

Enhanced Production of Soluble Recombinant Proteins With an In Situ-Removable Fusion Partner in a Cell-Free Synthesis System

2017 ◽  
Vol 12 (11) ◽  
pp. 1700125 ◽  
Author(s):  
Devi Kasi ◽  
Hee Ju Nah ◽  
Christy Catherine ◽  
Eung-Soo Kim ◽  
Kyubeom Han ◽  
...  
Keyword(s):  
Recombinant Proteins ◽  
Fusion Partner ◽  
Synthesis System ◽  
Enhanced Production ◽  
A Cell

Rapid Diversity-Oriented Synthesis in Microtiter Plates for In Situ Screening of HIV Protease Inhibitors

ChemBioChem ◽  
2003 ◽  
Vol 4 (11) ◽  
pp. 1246-1248 ◽  
Author(s):  
Ashraf Brik ◽  
John Muldoon ◽  
Ying-Chuan Lin ◽  
John H. Elder ◽  
David S. Goodsell ◽  
...  
Keyword(s):  
Protease Inhibitors ◽  
Hiv Protease ◽  
Hiv Protease Inhibitors ◽  
Diversity Oriented Synthesis ◽  
Microtiter Plates
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